Overview

  • Product name
    Mouse IgM [B11/7] - Isotype control
    See all Mouse isotype controls
  • Specificity
    ab91545 was produced against a synthetic hapten, which is normally not present in Humans or animals. Therefore, ab91545 does not react with any antigen of Human or animal cells.
  • Tested applications
    Suitable for: Flow Cytmore details
  • Immunogen

    Vegetal protein

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C. Store In the Dark.
  • Storage buffer
    Preservative: 0.1% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.2
  • Concentration information loading...
  • Purity
    Protein G purified
  • Clonality
    Monoclonal
  • Clone number
    B11/7
  • Isotype
    IgM
  • Research areas
  • Alternative names
    • Mouse Isotype Control

Applications

Our Abpromise guarantee covers the use of ab91545 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use 2µg for 106 cells. ab91545 is a negative isotype control

Images

  • Overlay histogram showing A549 cells stained with ab2784 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab2784, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgM [ICIGM] (ab91545, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in A549 cells fixed with 80% methanol (5 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.

References

ab91545 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-3 of 3 Q&A

Answer

Thank you for contacting us.

We carry several isotype controls that are mouse IgM:

ab91545: https://www.abcam.com/Mouse-IgM-ICIGM-Isotype-control-ab91545.html

ab18400: https://www.abcam.com/Mouse-IgM-kappa-MM-30-low-endotoxin-isotype-control-ab18400.html

ab18401: https://www.abcam.com/Mouse-IgM-kappa-monoclonal-MM-30-isotype-control-ab18401.html

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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Answer

Thank you for your reply.

All 3 isotype controls are mouse monoclonal antibodies.

Two of them have a kappa light chain (ab18400 and ab18401). For ab91545, we do not have information about the type of light chain (either kappa or lambda).

ICIGM is the clone number. All clone numbers are given in brackets [...] in our catalog.

The antibodiesdiffer in the tested applications, concentration and buffer composition and amount you would receive (100 ug or 500 ug).

You might want to choose the one of these 3 controls that comes closest to your primary antibody in terms of concentration, but in general any of these isotype controls would be suitable for your IHC experiment.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Use our products? Submit an Abreview. Earn rewards!
https://www.abcam.com/abreviews

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Answer

The testing discount promotion only applies to primary antibodies, defined as antibodies that are expected to produce a signal of some sort. For isotype controls such as ab91545, we will offer our guarantee for IHC, even though it is not listed on the datasheet, and so we do not offer the testing discount. If you or your customer have any questions, please contact us.

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