Overview

  • Product name

    Mouse IgM ELISA Kit
    See all IgM kits
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Low conc. 16 13.9pg/ml 6.7%
    Medium conc. 16 59.8pg/ml 3.1%
    High conc. 16 218.9pg/ml 2%
    Inter-assay
    Sample n Mean SD CV%
    Low conc. 8 11.3pg/ml 8.5%
    Medium conc. 8 58.2pg/ml 5%
    High conc. 8 201pg/ml 5.6%
  • Sample type

    Cell culture supernatant, Serum, Ascites Fluid
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    0.6 ng/ml
  • Range

    3.91 ng/ml - 250 ng/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Serum 102.8 % - %
    Tissue Culture Media 92.9 % - %
    Ascites Fluid 94.6 % - %

  • Assay time

    0h 90m
  • Assay duration

    Multiple steps standard assay
  • Species reactivity

    Reacts with: Mouse
    Predicted to work with: Rat
  • Product overview

    Abcam’s Mouse IgM in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of Mouse IgM in Tissue culture media, serum and ascites fluids.

    IgM specific antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells and along with an HRP-conjugated IgM detection antibody and the microplate is then incubated at room temperature. After the removal of unbound proteins by washing, TMB is used to visualize the HRP enzymatic reaction. TMB is catalyzed by HRP to produce a colored product that changes after adding acidic stop solution. The density of coloration is directly proportional to the IgM amount of sample captured in plate.

  • Notes

    IgM is one of the most primitive and least specialized immunoglobulins. It is one of the five classes of antibodies found in mammals and it contains µ class heavy chains. The half-life of IgM is 5 days and it has a high molecular weight of 900,000 Daltons, which prevents its passage into extravascular areas. IgM levels are dependent on the extent of antigenic stimulation from the environment3. A developing B cell always makes IgM before any other class of antibody. IgM formed in B cells is a four-chain molecule composed of two light chains and two heavy chains. Once IgM molecules are formed in B cells, they are inserted into the plasma membrane where they act as antigen receptors. IgM is also the major antibody class involved in a primary antibody response, during which IgM molecules are secreted into the blood in the form of a pentamer. In a pentamer, five four-chain IgM units are held together by disulfide bonds and a small glycopeptide known as a J (joining) chain, giving the molecule a total of 10 antigen binding sites.

     

    CompoundCross reactivity
    mouse IgM100%
    rat IgM60%
    Human IgM<0.4%
    mouse IgG1<0.2%
    mouse IgG2a<0.1%
    mouse IgG2b<0.1%
    mouse IgG3<0.1%
  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Microplate

Properties

  • Storage instructions

    Please refer to protocols.
  • Components 1 x 96 tests
    20X Wash Buffer Concentrate 1 x 100ml
    Assay Buffer 13 Concentrate 1 x 50ml
    Goat anti-mouse IgM Microplate (12 x 8 wells) 1 unit
    Mouse IgM Horseradish Peroxidase Conjugate 1 x 6ml
    Mouse IgM Standard 2 vials
    Plate Sealer 2 units
    Stop Solution 2 1 x 11ml
    TMB Substrate 1 x 12ml
  • Research areas

  • Relevance

    IgM normally constitutes about 10% of serum immunoglobulins. IgM antibody is prominent in early immune responses to most antigens and predominates in certain antibody responses such as 'natural' blood group antibodies. IgM (with IgD) is the major immunoglobulin expressed on the surface of B cells. The gene for the mu constant region contains four domains separated by short intervening sequences. Class specific anti immunoglobulin antibodies are useful for: The characterization of malignant B cell proliferations. All but acute lymphocytic leukemias share either surface or intra cytoplasmic Ig with an isotypic restriction, which suggest the monoclonal nature of the cell population. Most of the chronic lymphocytic leukemias, non Hodgkin lymphomas and Burkitt's lymphoma bear surface IgM, whereas plasmocytes from Waldenström's disease bear intracytoplasmic IgM. The other isotypes are less frequently found. On the other hand multiple myelomas are usually of the IgG or IgA type. Characterization of plasma cells in inflammatory conditions: Plasma cell typing can be of use for the classification of intestinal inflammatory conditions such as inflammatory bowel disease and allergic conditions. In the latter a specific increase in the number of IgE plasma cells can be demonstrated.
  • Alternative names

    • AGM1
    • Constant region of heavy chain of IgM
    • DKFZp686I15196
    • DKFZp686I15212
    • FLJ00385
    • Hepatitis B virus receptor binding protein
    • Ig mu chain C region
    • Immunoglobulin heavy constant mu
    • Immunoglobulin M
    • Immunoglobulin mu chain
    • MGC104996
    • MGC52291
    • MU
    • VH
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab133047 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • Mouse IgM measured in biological fluids, background signal subtracted (duplicates +/- SD).
  • Equal amounts of mouse monoclonal antibodies of known isotype were compared to the IgM mAb (ab84041) and expressed as % of IgM quantity, determined after background signal was subtracted (duplicates +/- SD).
  • Representative Standard Curve using ab133047.

Protocols

References

This product has been referenced in:

  • Rydahl MG  et al. Development of novel monoclonal antibodies against starch and ulvan - implications for antibody production against polysaccharides with limited immunogenicity. Sci Rep 7:9326 (2017). Read more (PubMed: 28839196) »
See 1 Publication for this product

Customer reviews and Q&As

Question
Answer

Samples in the majority of Tissue Culture Media, including those containing fetal bovine serum, can also be read in the assay, provided the standards have been diluted into the Tissue Culture Media instead of Assay Buffer 13. There will be a small change in binding associated with running the standards and samples in media. Users should only use standard curves generated in media or buffer to calculate concentrations of mouse IgM in the appropriate matrix.

Read More

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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