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Mouse IgM, kappa monoclonal [MM-30] - isotype control (ab18401)

Q&A (2)References (6)

Overview

  • Product name

    Mouse IgM, kappa monoclonal [MM-30] - isotype control
    See all Mouse isotype controls

  • Tested applications

    Suitable for: IHC-P, IHC-Fr, WB, IP, Flow Cytmore details

  • Immunogen

    KLH

  • General notes

    This immunoglobulin reacts with KLH.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C.

  • Storage buffer

    pH: 7.20
    Preservative: 0.09% Sodium azide
    Constituent: PBS
  • Concentration information loading...

  • Purification notes

    The immunoglobulin was purified by affinity chromatography.

  • Isotype control notes

    This immunoglobulin reacts with KLH.

  • Clonality

    Monoclonal

  • Clone number

    MM-30

  • Isotype

    IgM

  • Light chain type

    kappa

  • Research areas

  • Alternative names

    • Mouse Isotype Control

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab18401 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P
Use at an assay dependent concentration.
IHC-Fr
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration.
IP
Use at an assay dependent concentration.
Flow Cyt
Use at an assay dependent concentration.

Suitable for surface or intracellular staining.
Notes
IHC-P
Use at an assay dependent concentration.
IHC-Fr
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration.
IP
Use at an assay dependent concentration.
Flow Cyt
Use at an assay dependent concentration.

Suitable for surface or intracellular staining.

References (6)

Publishing research using ab18401? Please let us know so that we can cite the reference in this datasheet.

ab18401 has been referenced in 6 publications.

  • Tao L  et al. Sulfated glycosaminoglycans and low-density lipoprotein receptor contribute to Clostridium difficile toxin A entry into cells. Nat Microbiol 4:1760-1769 (2019). PubMed: 31160825
  • Klaus T  et al. Mouse Antibody of IgM Class is Prone to Non-Enzymatic Cleavage between CH1 and CH2 Domains. Sci Rep 8:519 (2018). PubMed: 29323348
  • Tian S  et al. Genome-wide CRISPR screens for Shiga toxins and ricin reveal Golgi proteins critical for glycosylation. PLoS Biol 16:e2006951 (2018). PubMed: 30481169
  • Klaus T  et al. Agglutinating mouse IgG3 compares favourably with IgMs in typing of the blood group B antigen: Functionality and stability studies. Sci Rep 6:30938 (2016). PubMed: 27484487
  • Karén J  et al. Effects of the histone deacetylase inhibitor valproic Acid on human pericytes in vitro. PLoS One 6:e24954 (2011). Flow Cyt . PubMed: 21966390
  • Sobol M  et al. UBF complexes with phosphatidylinositol 4,5-bisphosphate in nucleolar organizer regions regardless of ongoing RNA polymerase I activity. Nucleus 4:478-86 (0). PubMed: 24513678

Customer reviews and Q&As

Q&A
Submit a question

1-2 of 2 Q&A

Question

What are the differences between then? I saw that same are Kappa ans one is ICIGM?





































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Answer

Thank you for your reply.

All 3 isotype controls are mouse monoclonal antibodies.

Two of them have a kappa light chain (ab18400 and ab18401). For ab91545, we do not have information about the type of light chain (either kappa or lambda).

ICIGM is the clone number. All clone numbers are given in brackets [...] in our catalog.

The antibodiesdiffer in the tested applications, concentration and buffer composition and amount you would receive (100 ug or 500 ug).

You might want to choose the one of these 3 controls that comes closest to your primary antibody in terms of concentration, but in general any of these isotype controls would be suitable for your IHC experiment.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Use our products? Submit an Abreview. Earn rewards!
https://www.abcam.com/abreviews

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Question

The CD3 antibody that I have here is not from ABCAM.

I am looking for a negative control IgM, to be used in immunhistochemistry slides.

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Answer

Thank you for contacting us.

We carry several isotype controls that are mouse IgM:

ab91545: https://www.abcam.com/Mouse-IgM-ICIGM-Isotype-control-ab91545.html

ab18400: https://www.abcam.com/Mouse-IgM-kappa-MM-30-low-endotoxin-isotype-control-ab18400.html

ab18401: https://www.abcam.com/Mouse-IgM-kappa-monoclonal-MM-30-isotype-control-ab18401.html

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Use our products? Submit an Abreview. Earn rewards!
https://www.abcam.com/abreviews

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