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What kind of tissue extracts has this kit been tested with?
Asked on Apr 12 2012
Thank you for your call yesterday and for your patience while I've been in touch with the lab regarding your enquiry.
We are still looking into the question of what kind of tissue ab100705 was tested with, and I will be back in touch with this information. However I did obtain some suggestions for using ab100747 and ab1007390 with tissue lysates, and I wanted to forward this on to you.
Our kits are very accommodating to many different samples types so lysates will likelywork, though we can't guarantee it.When testing a kit with tissue lysates, we recommend diluting the samples at least 5-fold with the diluent or cell culture media to minimize any effects of the detergents in the lysis buffer.The samples may need to be diluted further but this would need to be determined empirically.
In brief, a lysis buffer must meet the following specifications:
A) has relatively low salt content (700 mM or less)
B) does not contain sodium azide
C) does not contain >0.1% SDS
D) does not contain >10 mM reducing agents (beta-mercaptoethanol or dithiothreitol)
This would include any buffers used for immunoprecipitations, including RIPA buffer.
I hope this information will be useful, but please let me know if you have any further questions or if there is anything else that we can do for you. I'll be back in touch once I have more information about ab100705.
Answered on Apr 12 2012