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I need to be able to detect both active and pro_IL-1beta from mouse cell culture supernatant and lysates.
> I am looking for a way to quantitatively detect active and proIL-1beta separately. I have an ELISA that can pick up the pro form on its own but now need either an ELISA that will pick up both equally or just the active form. Do you have any products that will help me?
Asked on Dec 19 2012
Thank you for your patience.
I am still waiting on more information on ab108866.
But I am happy to provide the following information about ab100704 and ab100705:
The antibody pair used in the Mouse IL-1 beta Sandwich ELISA kits was raised against an immunogen which was the mature/cleaved form of the IL-1 beta protein consisting of amino acids 118-269. Therefore we know that the kit can detect the active/mature form.
Whether the kit can also detect the propeptide however has not been determined so accordingly we don’t know if this kit can differentiate between the propeptide and the mature form. The only way to determine this would be to do either epitope mapping (which we haven’t done as it is time-consuming and costly) or to simply spike in only the propeptide and see if the kit detects it.
Because the propeptide does contain the amino acid sequence of the mature peptide though, if it’s a linear epitope then likely the kit would also detect the propeptide. If the epitope is confirmational or some post-translational modification blocks the binding site, then the kit would likely not detect the propeptide). This is all speculation of course because we don’t know the epitope.
I hope this information is helpful. Please do not hesitate to contact em again with any further questions.
Answered on Dec 19 2012