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thnx for reply. as the non availity of chromogen substrate i have used p-npp as substrate for colour development & for that i have used suitable secondary antibody from my friend's lab.i want to know how much difference found in standard curve in the respective cases (O.D. of p-npp vs conc. of IL1-b & O.D. of chromogen substrate vs conc. of IL-1b).pls send me the std curve of O.D. of p-npp vs conc. of IL1-b by mail.
Asked on Aug 16 2011
Thank you for contacting us. Please be advised that we have not done any test with p-NPP substrate so we will not be able to provide any data regarding this. We have only tested this Kit using the reagent mentioned on the KIT datasheet. Regarding the standard curve customers have to prepare standard curve by themselves. We have provided everything in the KIT for the preparation of standard curve. Please check in literature what standard curve really mean and then prepare it following the protocol attached to the datasheet. http://en.wikipedia.org/wiki/Standard_curve http://www.graphpad.com/curvefit/introduction5b.htm I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.
Answered on Aug 16 2011