Question (70310) | Mouse IL-1 beta ELISA kit (ab108866)

Go to datasheet (ab108866)

Question


Thanks for your reply. The problem we have here is that we believe that the ELISA kit we have in at the moment (ebioscience kit) detects both mouse active and mouse pro IL-1b at different affinities. In more detail, we think that the active IL-1b is detected around 10X better than the pro-IL-1beta. Obviously this is not much good to our lab and so we need a new kit to measure both. It is however, it is not financially viable for me to order in a whole range of kits from different companies in order to find a kit that will detect btw forms of the cytokine equally. Instead I was hoping that you may be able to give me a sample of your kit. In return, we could provide some information on the specificity of the ELISA. As an additional incentive to offer a sample, we do a lot of IL-1b work in our lab and so, if the kit does detect both equally, we would most likely order many more kits in the future. I hope you understand our situation.
Many thanks

Answer

Thank you for your reply.


Because we carry over 100,000 products, it isn't feasible for us to keep small sample sizes of our products.

We are happy to reassure our customers that all of our products are covered by our Abpromise, which guarantees that the product will work in the applications and species specified on the datasheet, or we will offer a replacement, credit, or refund within 6 months of purchase.


Regrettably, as we haven't tested or even compared the reactivity to the pro- or mature form of the IL1 beta, we cannot guarantee it will work with both forms.

However, we are still waiting for a response from our collaborator of ab108866. So there is still a change that the reactivity has been measured.


I hope this information is still helpful. We will let you know as soon as we got a reply.