Overview

  • Product name

    Mouse IL-1 RL1 ELISA Kit (ST2)
    See all ST2 kits
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    1 16 0.57ng/ml 0.029 5.1%
    2 16 2.33ng/ml 0.086 3.7%
    3 16 4.25ng/ml 0.136 3.2%
    Inter-assay
    Sample n Mean SD CV%
    1 24 0.68ng/ml 0.058 8.6%
    2 24 2.74ng/ml 0.17 6.2%
    3 24 4.61ng/ml 0.244 5.3%
  • Sample type

    Cell culture supernatant, Serum, Cell Lysate, Heparin Plasma, EDTA Plasma, Tissue Homogenate
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    < 10 pg/ml
  • Range

    156 pg/ml - 10000 pg/ml
  • Assay time

    3h 30m
  • Assay duration

    Multiple steps standard assay
  • Species reactivity

    Reacts with: Mouse
  • Product overview

    The Mouse IL1RL1 Enzyme-Linked Immunosorbent Assay (ELISA) kit (ab213871) is designed for the quantitative measurement of Mouse IL1RL1 in cell culture supernatants, cell lysates, tissue homogenates, serum and plasma (heparin, EDTA).


    The ELISA kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for IL1RL1 has been pre-coated onto 96-well plates. Standards (Expression system for standard: NSO; Immunogen sequence: S27-R332) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IL1RL1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB is used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Mouse IL1RL1 amount of sample captured in plate.

  • Notes

    Interleukin 1 receptor-like 1, also known as IL1RL1 or ST2, is a protein that in humans is encoded by the IL1RL1 gene. The protein encoded by this gene is a member of the interleukin 1 receptor family. Studies of the similar gene in mouse suggested that this receptor can be induced by proinflammatory stimuli, and may be involved in the function of helper T cells. IL1RL1 is necessary for endotoxin tolerance and, by inhibiting TLR responses, enhances Th2 responses. This gene, interleukin 1 receptor, type I (IL1R1), interleukin 1 receptor, type II (IL1R2) and interleukin 1 receptor-like 2 (IL1RL2) form a cytokine receptor gene cluster in a region mapped to chromosome 2q12.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Pre-coated microplate (12 x 8 well strips)

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components Identifier 1 x 96 tests
    ABC Diluent Buffer Blue Cap 1 x 12ml
    Adhesive Plate Seal 4 units
    Antibody Diluent Buffer Green Cap 1 x 12ml
    Anti-Mouse IL1RL1 coated Microplate (12 x 8 wells) 1 unit
    Avidin-Biotin-Peroxidase Complex (ABC) 1 x 130µl
    Biotinylated anti- Mouse IL1RL1 antibody 1 x 130µl
    Lyophilized recombinant Mouse IL1RL1 standard 2 vials
    Sample Diluent Buffer Green Cap 1 x 30ml
    TMB Color Developing Agent Black Cap 1 x 10ml
    TMB Stop Solution Yellow Cap 1 x 10ml
  • Research areas

  • Function

    Receptor for interleukin-33 (IL-33), its stimulation recruits MYD88, IRAK1, IRAK4, and TRAF6, followed by phosphorylation of MAPK3/ERK1 and/or MAPK1/ERK2, MAPK14, and MAPK8. Possibly involved in helper T-cell function.
  • Tissue specificity

    Highly expressed in kidney, lung, placenta, stomach, skeletal muscle, colon and small intestine. Isoform A is prevalently expressed in the lung, testis, placenta, stomach and colon. Isoform B is more abundant in the brain, kidney and the liver. Isoform C is not detected in brain, heart, liver, kidney and skeletal muscle.
  • Sequence similarities

    Belongs to the interleukin-1 receptor family.
    Contains 3 Ig-like C2-type (immunoglobulin-like) domains.
    Contains 1 TIR domain.
  • Cellular localization

    Secreted and Cell membrane.
  • Information by UniProt
  • Alternative names

    • DER-4
    • DER4
    • FIT 1
    • Growth stimulation expressed
    • homolog of mouse growth stimulation-expressed
    • Il1rl1
    • IL33R
    • ILRL1_HUMAN
    • Interleukin 1 receptor like 1
    • interleukin 1 receptor related protein
    • Interleukin-1 receptor-like 1
    • Protein ST2
    • ST2
    • ST2L
    • ST2V
    • T1
    • T1 protein
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab213871 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • Mouse IL1RL1 ELISA Kit (ab213871) Standard Curve.

Protocols

References

ab213871 has not yet been referenced specifically in any publications.

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