Key features and details
- Sensitivity: 1 ng/ml
- Range: 1.85 ng/ml - 450 ng/ml
- Sample type: Cell culture supernatant, Plasma
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
Product nameMouse IL-11 ELISA Kit
See all IL-11 kits
Sample typeCell culture supernatant, Plasma
Assay typeSandwich (quantitative)
Sensitivity< 1 ng/ml
Range1.85 ng/ml - 450 ng/ml
Sample specific recovery Sample type Average % Range Cell culture supernatant 77.35 70% - 87% Plasma 117.3 95% - 113%
Assay durationMultiple steps standard assay
Species reactivityReacts with: Mouse
Abcam’s IL-11 Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse IL-11 in plasma and cell culture supernatants. (Mouse IL-11 concentration is pretty low in normal plasma, it may not be detected in this assay).
This assay employs an antibody specific for mouse IL-11 coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-11 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse IL-11 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-11 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
We have not been able to detect the endogenous Mouse IL-11 in normal serum with ab100698, only in serum spiked with Mouse IL-11.
Storage instructionsStore at -20°C. Please refer to protocols.
Components 1 x 96 tests 20X Wash Buffer Concentrate 1 x 25ml 400X HRP-Streptavidin Concentrate 1 x 200µl 5X Assay Diluent B 1 x 15ml 5X Assay Diluent D 1 x 15ml Biotinylated anti-mouse IL-11 2 vials IL-11 Microplate (12 x 8 wells) 1 unit Recombinant Mouse IL-11 Standard (lyophilized) 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml
FunctionDirectly stimulates the proliferation of hematopoietic stem cells and megakaryocyte progenitor cells and induces megakaryocyte maturation resulting in increased platelet production.
Sequence similaritiesBelongs to the IL-6 superfamily.
- Information by UniProt
- Adipogenesis inhibitory factor
- IL 11
ab100698 has been referenced in 1 publication.
- Buzzelli JN et al. Overexpression of IL-11 promotes premalignant gastric epithelial hyperplasia in isolation from germline gp130-JAK-STAT driver mutations. Am J Physiol Gastrointest Liver Physiol 316:G251-G262 (2019). PubMed: 30520693