Overview

  • Product name

    Mouse IL-11 ELISA Kit
    See all IL-11 kits
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Overall 8 5.4%
    Inter-assay
    Sample n Mean SD CV%
    Overall 3 10.3%
  • Sample type

    Cell culture supernatant, Serum, Cit plasma
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    17.4 pg/ml
  • Range

    78.12 pg/ml - 5000 pg/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 102 98% - 105%
    Serum 100 98% - 101%
    Cit plasma 91 86% - 97%

  • Assay time

    1h 30m
  • Assay duration

    One step assay
  • Species reactivity

    Reacts with: Mouse
    Does not react with: Cow
  • Product overview

    IL-11 in vitro SimpleStep ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of IL-11 protein in mouse serum, citrate plasma, and cell culture supernatants.


    The SimpleStep ELISA employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material. TMB substrate is added and during incubation is catalyzed by HRP, generating blue coloration. This reaction is then stopped by addition of Stop Solution completing any color change from blue to yellow. Signal is generated proportionally to the amount of bound analyte and the intensity is measured at 450 nm. Optionally, instead of the endpoint reading, development of TMB can be recorded kinetically at 600 nm.

  • Notes

    IL-11 is a multifunctional cytokine that is a key regulator of hematopoiesis. It stimulates hematopoietic stem cell and megakaryocyte progenitor cell proliferation. It is also involved in the response to liver damage by inducing proliferation of hepatocytes.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Pre-coated microplate (12 x 8 well strips)

Properties

Applications

Our Abpromise guarantee covers the use of ab215084 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

     

  • Background-subtracted data values (mean +/- SD) are graphed.

  • The concentrations of IL-11 were measured in duplicates and interpolated from the IL-11 standard curves. Undiluted samples are as follows: lung supernatant stimulated with TPA and IL-1a 100%. The interpolated values are plotted (mean +/- SD, n=2). The mean IL-11 concentration was determined to be 2143 pg/mL in lung supernatant stimulated with TPA and IL-1a.

  • Conditioned media was harvested after 48 hours. IL-11 was measured in 100% unstimulated and stimulated lung supernatant. The concentrations of IL-11 were measured in duplicate, interpolated from the IL-11 standard curves. The interpolated values are plotted (mean +/- SD, n=2). The mean IL-11 concentration was determined to be 1849 pg/mL in TPA and IL-1a stimulated lung supernatant and 809 pg/mL in unstimulated lung supernatant.

Protocols

References

ab215084 has not yet been referenced specifically in any publications.

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