Mouse IL-11 ELISA Kit (ab215084)
- Datasheet
- References
- Protocols
Overview
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Product name
Mouse IL-11 ELISA Kit
See all IL-11 kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall 8 5.4% Inter-assay Sample n Mean SD CV% Overall 3 10.3% -
Sample type
Cell culture supernatant, Serum, Cit plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
17.4 pg/ml -
Range
78.12 pg/ml - 5000 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Cell culture supernatant 102 98% - 105% Serum 100 98% - 101% Cit plasma 91 86% - 97% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Mouse
Does not react with: Cow -
Product overview
IL-11 in vitro SimpleStep ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of IL-11 protein in mouse serum, citrate plasma, and cell culture supernatants.
The SimpleStep ELISA employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material. TMB substrate is added and during incubation is catalyzed by HRP, generating blue coloration. This reaction is then stopped by addition of Stop Solution completing any color change from blue to yellow. Signal is generated proportionally to the amount of bound analyte and the intensity is measured at 450 nm. Optionally, instead of the endpoint reading, development of TMB can be recorded kinetically at 600 nm.
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Notes
IL-11 is a multifunctional cytokine that is a key regulator of hematopoiesis. It stimulates hematopoietic stem cell and megakaryocyte progenitor cell proliferation. It is also involved in the response to liver damage by inducing proliferation of hepatocytes.
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Tested applications
Suitable for: Sandwich ELISAmore details -
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Wash Buffer PT (ab206977) 1 x 20ml Antibody Diluent CPR 1 x 6ml 10X Mouse IL-11 Capture Antibody 1 x 600µl 10X Mouse IL-11 Detector Antibody 1 x 600µl Mouse IL-11 Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Sample Diluent NS (ab193972) 1 x 50ml Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
Directly stimulates the proliferation of hematopoietic stem cells and megakaryocyte progenitor cells and induces megakaryocyte maturation resulting in increased platelet production. -
Sequence similarities
Belongs to the IL-6 superfamily. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- Adipogenesis inhibitory factor
- AGIF
- IL 11
see all -
Database links
- Entrez Gene: 16156 Mouse
- SwissProt: P47873 Mouse
- Unigene: 35814 Mouse
Associated products
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ELISA kits
Applications
Our Abpromise guarantee covers the use of ab215084 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Sandwich ELISA | Use at an assay dependent concentration. |
Images
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Other - Mouse IL-11 ELISA Kit (ab215084)SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Example of mouse IL-11 standard curve in Sample Diluent NS.Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of native IL-11 in mouse lung cell culture supernatant samples.The concentrations of IL-11 were measured in duplicates and interpolated from the IL-11 standard curves. Undiluted samples are as follows: lung supernatant stimulated with TPA and IL-1a 100%. The interpolated values are plotted (mean +/- SD, n=2). The mean IL-11 concentration was determined to be 2143 pg/mL in lung supernatant stimulated with TPA and IL-1a.
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Mouse lung cells were cultured unstimulated or stimulated with 10 ng/mL TPA and 1 ng/mL IL-1a.Conditioned media was harvested after 48 hours. IL-11 was measured in 100% unstimulated and stimulated lung supernatant. The concentrations of IL-11 were measured in duplicate, interpolated from the IL-11 standard curves. The interpolated values are plotted (mean +/- SD, n=2). The mean IL-11 concentration was determined to be 1849 pg/mL in TPA and IL-1a stimulated lung supernatant and 809 pg/mL in unstimulated lung supernatant.
Protocols
References
ab215084 has not yet been referenced specifically in any publications.
