Mouse IL-12 p40 + IL-12 p70 ELISA Kit (ab100699)
Key features and details
- Sensitivity: 5 pg/ml
- Range: 2.74 pg/ml - 2000 pg/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
Overview
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Product name
Mouse IL-12 p40 + IL-12 p70 ELISA Kit -
Detection method
Colorimetric -
Sample type
Cell culture supernatant, Serum, Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
< 5 pg/ml -
Range
2.74 pg/ml - 2000 pg/ml -
Recovery
94 %
Sample specific recovery Sample type Average % Range Cell culture supernatant 95.25 84% - 103% Serum 93.29 82% - 102% Plasma 94.52 83% - 103% -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Mouse -
Product overview
Abcam’s IL-12 p40 + IL-12 p70 Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Mouse IL-12 p40 + IL-12 p70 in serum, plasma and cell culture supernatants.
This assay employs an antibody specific for Mouse IL-12 p40 + IL-12 p70 coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-12 p40 + IL-12 p70 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-IL-12 p40 + IL-12 p70 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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Notes
The standard is affinity purified.
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Platform
Microplate
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 20X Wash Buffer 1 x 25ml 400X HRP-Streptavidin Concentrate 1 x 200µl 5X Assay Diluent B 1 x 15ml Assay Diluent A 1 x 30ml Biotinylated anti-Mouse IL-12 p40/p70 2 vials IL-12 p40/p70 Microplate (12 x 8 wells) 1 unit Recombinant Mouse IL-12 p40/p70 Standard (lyophilized) 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml -
Research areas
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Relevance
IL-12 is a heterodimeric cytokine comprised of p35 and p40 subunits. IL-12 plays a central role in mediating cell mediated immunity, promoting differentiation of CD4+ T cells to the Th1 subset and of CD8+ T cells into mature cytotoxic T lymphocytes (CTLs). IL-12 is a potent stimulator of NK cells. IL-12 therapy has been suggested as a method of enhancing cytocidal anti tumour immune responses. -
Alternative names
- CLMF p35
- CLMF p40
- CLMF1
see all -
Database links
- Entrez Gene: 16159 Mouse
- Entrez Gene: 16160 Mouse
- SwissProt: P43431 Mouse
- SwissProt: P43432 Mouse
Datasheets and documents
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SDS download
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Datasheet download
References (3)
ab100699 has been referenced in 3 publications.
- Zhang Z et al. Suppression of Mll1-Complex by Stat3/Cebpß-Induced miR-21a/21b/181b Maintains the Accumulation, Homeostasis, and Immunosuppressive Function of Polymorphonuclear Myeloid-Derived Suppressor Cells. J Immunol 204:3400-3415 (2020). PubMed: 32358019
- Kong R & Yin J Efficacy of subcutaneous immunotherapy in a mouse model of Humulus pollen-induced allergic asthma. Am J Transl Res 11:280-289 (2019). PubMed: 30787986
- Yi R et al. Isolation and Identification of Lactobacillus plantarum HFY05 from Natural Fermented Yak Yogurt and Its Effect on Alcoholic Liver Injury in Mice. Microorganisms 7:N/A (2019). PubMed: 31694208