Overview

  • Product name

    Mouse IL-12 p40 + IL-12 p70 ELISA Kit
  • Detection method

    Colorimetric
  • Sample type

    Cell culture supernatant, Serum, Plasma
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    < 5 pg/ml
  • Range

    2.74 pg/ml - 2000 pg/ml
  • Recovery

    94 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 95.25 84% - 103%
    Serum 93.29 82% - 102%
    Plasma 94.52 83% - 103%

  • Assay duration

    Multiple steps standard assay
  • Species reactivity

    Reacts with: Mouse
  • Product overview

    Abcam’s IL-12 p40 + IL-12 p70 Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Mouse IL-12 p40 + IL-12 p70 in serum, plasma and cell culture supernatants.


    This assay employs an antibody specific for Mouse IL-12 p40 + IL-12 p70 coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-12 p40 + IL-12 p70 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-IL-12 p40 + IL-12 p70 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Microplate

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components 1 x 96 tests
    20X Wash Buffer Concentrate 1 x 25ml
    400X HRP-Streptavidin Concentrate 1 x 200µl
    5X Assay Diluent B 1 x 15ml
    Assay Diluent A 1 x 30ml
    Biotinylated anti-Mouse IL-12 p40/p70 (lyophilized) 2 vials
    IL-12 p40/p70 Microplate (12 x 8 wells) 1 unit
    Recombinant Mouse IL-12 p40/p70 Standard (lyophilized) 2 vials
    Stop Solution 1 x 8ml
    TMB One-Step Substrate Reagent 1 x 12ml
  • Research areas

  • Relevance

    IL-12 is a heterodimeric cytokine comprised of p35 and p40 subunits. IL-12 plays a central role in mediating cell mediated immunity, promoting differentiation of CD4+ T cells to the Th1 subset and of CD8+ T cells into mature cytotoxic T lymphocytes (CTLs). IL-12 is a potent stimulator of NK cells. IL-12 therapy has been suggested as a method of enhancing cytocidal anti tumour immune responses.
  • Alternative names

    • CLMF p35
    • CLMF p40
    • CLMF1
    • CLMF2
    • Cytotoxic lymphocyte maturation factor 1, p35
    • Cytotoxic lymphocyte maturation factor 35 kDa subunit
    • Cytotoxic lymphocyte maturation factor 40 kDa subunit
    • Il 12b
    • Il 12p40
    • IL12 p40 + IL12 p70
    • IL12 subunit p35
    • IL12 subunit p40
    • IL12A
    • IL12B
    • Il12p40, p40
    • Interleukin 12 alpha chain
    • Interleukin 12 subunit alpha
    • Interleukin 12 subunit beta
    • Interleukin 12A (natural killer cell stimulatory factor 1, cytotoxic lymphocyte maturation factor 1, p35)
    • Interleukin 12B (natural killer cell stimulatory factor 2, cytotoxic lymphocyte maturation factor 2, p40)
    • MGC151228
    • MGC151232
    • Natural killer cell stimulatory factor 1, 35 kD subunit
    • Natural killer cell stimulatory factor, 40 kD subunit
    • NKSF
    • NKSF2 antibody
    • OTTMUSP00000005619
    • P35
    • RP23-388G23.1
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab100699 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • Representative standard curve using ab100699

  • Representative standard curve using ab100699

Protocols

References

This product has been referenced in:

  • Kong R & Yin J Efficacy of subcutaneous immunotherapy in a mouse model of Humulus pollen-induced allergic asthma. Am J Transl Res 11:280-289 (2019). Read more (PubMed: 30787986) »
See 1 Publication for this product

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