Key features and details
- Sensitivity: 5 pg/ml
- Range: 2.74 pg/ml - 2000 pg/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
Product nameMouse IL-12 p40 + IL-12 p70 ELISA Kit
Sample typeCell culture supernatant, Serum, Plasma
Assay typeSandwich (quantitative)
Sensitivity< 5 pg/ml
Range2.74 pg/ml - 2000 pg/ml
Sample specific recovery Sample type Average % Range Cell culture supernatant 95.25 84% - 103% Serum 93.29 82% - 102% Plasma 94.52 83% - 103%
Assay durationMultiple steps standard assay
Species reactivityReacts with: Mouse
Abcam’s IL-12 p40 + IL-12 p70 Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Mouse IL-12 p40 + IL-12 p70 in serum, plasma and cell culture supernatants.
This assay employs an antibody specific for Mouse IL-12 p40 + IL-12 p70 coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-12 p40 + IL-12 p70 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-IL-12 p40 + IL-12 p70 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Storage instructionsStore at -20°C. Please refer to protocols.
Components 1 x 96 tests 20X Wash Buffer Concentrate 1 x 25ml 400X HRP-Streptavidin Concentrate 1 x 200µl 5X Assay Diluent B 1 x 15ml Assay Diluent A 1 x 30ml Biotinylated anti-Mouse IL-12 p40/p70 (lyophilized) 2 vials IL-12 p40/p70 Microplate (12 x 8 wells) 1 unit Recombinant Mouse IL-12 p40/p70 Standard (lyophilized) 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml
RelevanceIL-12 is a heterodimeric cytokine comprised of p35 and p40 subunits. IL-12 plays a central role in mediating cell mediated immunity, promoting differentiation of CD4+ T cells to the Th1 subset and of CD8+ T cells into mature cytotoxic T lymphocytes (CTLs). IL-12 is a potent stimulator of NK cells. IL-12 therapy has been suggested as a method of enhancing cytocidal anti tumour immune responses.
- CLMF p35
- CLMF p40
ab100699 has been referenced in 1 publication.
- Kong R & Yin J Efficacy of subcutaneous immunotherapy in a mouse model of Humulus pollen-induced allergic asthma. Am J Transl Res 11:280-289 (2019). PubMed: 30787986