Overview

  • Product name

    Mouse IL-12 p70 ELISA Kit
    See all IL12 p70 kits
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    1 16 97pg/ml 6.5 6.7%
    2 16 367pg/ml 25.7 7%
    3 16 633pg/ml 34.2 5.4%
    Inter-assay
    Sample n Mean SD CV%
    1 24 123pg/ml 9.23 7.5%
    2 24 408pg/ml 33.05 8.1%
    3 24 657pg/ml 38.8 5.9%
  • Sample type

    Cell culture supernatant, Serum
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    < 1.5 pg/ml
  • Range

    15.6 pg/ml - 1000 pg/ml
  • Assay time

    3h 30m
  • Assay duration

    Multiple steps standard assay
  • Species reactivity

    Reacts with: Mouse
  • Product overview

    The Mouse IL-12 p70 Enzyme-Linked Immunosorbent Assay (ELISA) kit (ab213866) is designed for the quantitative measurement of Mouse IL-12 p70 in cell culture supernatants and serum.


    The ELISA kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for IL-12 p70 has been pre-coated onto 96-well plates. Standards (Expression system for standard: sf21; Immunogen sequence: (M23-S335) + (R23-A215)) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IL-12 p70 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB is used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Mouse IL-12 p70 amount of sample captured in plate.

  • Notes

    Interleukin(IL)-12 is a 70-KDa cytokine comprised of two disulfide-linked proteins (p35 and p40) and is essential for the initiation of effective immune response. IL-12 p70 is a heterodimer of p35 and p40 subunits; it is an important cytokine secreted by antigen-presenting cells in response to antigenic stimulation. Gene expression analysis of the IL-12 cytokine family subunits revealed that both strains induced high levels of p40 (protein chain communal to IL-12 p70 and IL-23) as well as p19, a subunit of IL-23. Conversely only ACT- 18HS19 infection induced consistent transcription of IL-12 p35, a subunit of IL-12 p70. The standard product used in this kit is recombinant mouse IL-12 p70 comprising of a 193 amino acids p35 chain and a 313 amino acids p40 chain connected by a disulfide bond.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Pre-coated microplate (12 x 8 well strips)

Properties

Applications

Our Abpromise guarantee covers the use of ab213866 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • Mouse IL-12 p70 ELISA Kit (ab213866) Standard Curve.

Protocols

References

ab213866 has not yet been referenced specifically in any publications.

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