Mouse IL-13 ELISA Kit (ab219634)
- Datasheet
- References
- Protocols
Overview
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Product name
Mouse IL-13 ELISA Kit
See all IL-13 kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% supernatant 5 2.2% Inter-assay Sample n Mean SD CV% supernatant 3 1.4% -
Sample type
Cell culture supernatant, Serum, Heparin Plasma, EDTA Plasma, Citrate Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
1.2 pg/ml -
Range
1.95 pg/ml - 125 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Cell culture supernatant 108 106% - 111% Serum 97 95% - 98% Heparin Plasma 104 101% - 108% EDTA Plasma 98 97% - 99% Citrate Plasma 97 95% - 100% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Mouse
Does not react with: Rat, Human -
Product overview
IL-13 in vitro SimpleStep ELISA® (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of IL-13 protein mouse in serum, plasma, and cell culture supernatant.
The SimpleStep ELISA® employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material. TMB substrate is added and during incubation is catalyzed by HRP, generating blue coloration. This reaction is then stopped by addition of Stop Solution completing any color change from blue to yellow. Signal is generated proportionally to the amount of bound analyte and the intensity is measured at 450 nm. Optionally, instead of the endpoint reading, development of TMB can be recorded kinetically at 600 nm.
Interleukin-13 (IL-13) is a secreted immunoregulatory cytokine in the IL-4/IL-13 family that is linked to allergic reactions and cancer pathogenesis. IL-13 inhibits inflammatory cytokine production and promotes immunoglobulin class switching on B-cells. IL-13 synergizes with IL2 in regulating interferon-gamma synthesis. IL-13 is linked to allergic rhinitis, a common disease of complex inheritance characterized by mucosal inflammation caused by allergen exposure.
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Tested applications
Suitable for: Sandwich ELISAmore details -
Platform
Microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Mouse IL-13 Capture Antibody 1 x 600µl 10X Mouse IL-13 Detector Antibody 1 x 600µl Mouse IL-13 Lyophilized Recombinant Protein 2 vials Antibody Diluent CPR 1 x 6ml 10X Wash Buffer PT (ab206977) 1 x 20ml Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml Sample Diluent NS 1 x 20ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Plate Seals 1 unit -
Research areas
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Function
Cytokine (PubMed:8096327, PubMed:8097324). Inhibits inflammatory cytokine production (PubMed:8096327). Synergizes with IL2 in regulating interferon-gamma synthesis (PubMed:8096327). May be critical in regulating inflammatory and immune responses (PubMed:8096327, PubMed:8097324). Positively regulates IL31RA expression in macrophages. -
Involvement in disease
Allergic rhinitis -
Sequence similarities
Belongs to the IL-4/IL-13 family. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- Allergic rhinitis
- ALRH
- BHR 1
see all -
Database links
- Entrez Gene: 16163 Mouse
- SwissProt: P20109 Mouse
- Unigene: 1284 Mouse
Associated products
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SimpleStep ELISA kits
Applications
Our Abpromise guarantee covers the use of ab219634 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Sandwich ELISA | Use at an assay dependent concentration. |
Images
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Other - Mouse IL-13 ELISA Kit (ab219634)SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Example of mouse IL-13 standard curve.Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of spike IL-13 in mouse serum and plasma samples.The concentrations of IL-13 were measured in duplicates, interpolated from the IL-13 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 10%, plasma (citrate) 10%, plasma (EDTA) 10% and plasma (heparin) 5%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
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Interpolated concentrations of native IL-13 in mouse serum and plasma samples.The concentrations of IL-13 were measured in duplicates, interpolated from the IL-13 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 10%, plasma (citrate) 10%, plasma (EDTA) 10% and plasma (heparin) 5%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IL-13 concentration was determined to be 31.5 pg/mL in serum, 21.1 pg/mL in plasma (citrate) and 20.4 pg/mL in plasma (EDTA) and 29.1 pg/mL in plasma (heparin).
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Interpolated concentrations of native IL-13 in mouse EL4/IL-2 cell culture supernatant unstimulated and PHA/PMA stimulated samples.The concentrations of IL-13 were measured in duplicates, interpolated from the IL-13 standard curves and corrected for sample dilution. Undiluted samples are 2%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). T The mean IL-13 concentration was determined to be 3144 pg/mL in PHA/PMA stimulated EL4/IL-2 cell culture supernatant and undetermined in media and unstimulated cell culture supernatant.
Protocols
References
ab219634 has not yet been referenced specifically in any publications.
