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    mouse-il-13-elisa-kit-ab219634.pdf

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Immunology Innate Immunity Cytokines Interleukins
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RecombinantSimpleStep

Mouse IL-13 ELISA Kit (ab219634)

  • Datasheet
  • SDS
  • Protocol Booklet
Reviews (1) Submit a question References (5)

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Other - Mouse IL-13 ELISA Kit (ab219634)
  • Example of mouse IL-13 standard curve.
  • Interpolated concentrations of spike IL-13 in mouse serum and plasma samples.
  • Interpolated concentrations of native IL-13 in mouse serum and plasma samples.
  • Interpolated concentrations of native IL-13 in mouse EL4/IL-2 cell culture supernatant unstimulated and PHA/PMA stimulated samples.
  • Sandwich ELISA - Mouse IL-13 ELISA Kit (ab219634)

Key features and details

  • One-wash 90 minute protocol
  • Sensitivity: 1.2 pg/ml
  • Range: 1.95 pg/ml - 125 pg/ml
  • Sample type: Cell culture supernatant, Cit plasma, EDTA Plasma, Hep Plasma, Serum
  • Detection method: Colorimetric
  • Assay type: Sandwich (quantitative)
  • Reacts with: Mouse

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Overview

  • Product name

    Mouse IL-13 ELISA Kit
    See all IL-13 kits
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    supernatant 5 2.2%
    Inter-assay
    Sample n Mean SD CV%
    supernatant 3 1.4%
  • Sample type

    Cell culture supernatant, Serum, Hep Plasma, EDTA Plasma, Cit plasma
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    1.2 pg/ml
  • Range

    1.95 pg/ml - 125 pg/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 108 106% - 111%
    Serum 97 95% - 98%
    Hep Plasma 104 101% - 108%
    EDTA Plasma 98 97% - 99%
    Cit plasma 97 95% - 100%
  • Assay time

    1h 30m
  • Assay duration

    One step assay
  • Species reactivity

    Reacts with: Mouse
  • Product overview

    Mouse IL-13 ELISA Kit (ab219634) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of IL-13 protein in cell culture supernatant, serum, cit plasma, edta plasma, and hep plasma. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse IL-13 with 1.2 pg/ml sensitivity.


    SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:


            - Single-wash protocol reduces assay time to 90 minutes or less
            - High sensitivity, specificity and reproducibility from superior antibodies
            - Fully validated in biological samples
            - 96-wells plate breakable into 12 x 8 wells strips


    A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

  • Notes

    Interleukin-13 (IL-13) is a secreted immunoregulatory cytokine in the IL-4/IL-13 family that is linked to allergic reactions and cancer pathogenesis. IL-13 inhibits inflammatory cytokine production and promotes immunoglobulin class switching on B-cells. IL-13 synergizes with IL2 in regulating interferon-gamma synthesis. IL-13 is linked to allergic rhinitis, a common disease of complex inheritance characterized by mucosal inflammation caused by allergen exposure.

  • Platform

    Microplate (12 x 8 well strips)

Properties

  • Storage instructions

    Store at +4°C. Please refer to protocols.
  • Components 1 x 96 tests
    10X Mouse IL-13 Capture Antibody 1 x 600µl
    10X Mouse IL-13 Detector Antibody 1 x 600µl
    10X Wash Buffer PT (ab206977) 1 x 20ml
    Antibody Diluent CPR 1 x 6ml
    Mouse IL-13 Lyophilized Recombinant Protein 2 vials
    Plate Seals 1 unit
    Sample Diluent NS (ab193972) 1 x 50ml
    SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit
    Stop Solution 1 x 12ml
    TMB Development Solution 1 x 12ml
  • Research areas

    • Immunology
    • Innate Immunity
    • Cytokines
    • Interleukins
    • Cancer
    • Tumor immunology
    • Cytokines
    • Interleukins
    • Kits/ Lysates/ Other
    • Kits
    • ELISA Kits
    • ELISA Kits
    • Cytokines and cytokine receptors ELISA kits
    • Cardiovascular
    • Angiogenesis
    • Endothelial Cell Markers
  • Function

    Cytokine (PubMed:8096327, PubMed:8097324). Inhibits inflammatory cytokine production (PubMed:8096327). Synergizes with IL2 in regulating interferon-gamma synthesis (PubMed:8096327). May be critical in regulating inflammatory and immune responses (PubMed:8096327, PubMed:8097324). Positively regulates IL31RA expression in macrophages.
  • Involvement in disease

    Allergic rhinitis
  • Sequence similarities

    Belongs to the IL-4/IL-13 family.
  • Cellular localization

    Secreted.
  • Target information above from: UniProt accession P35225 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Alternative names

    • Allergic rhinitis
    • ALRH
    • BHR 1
    • BHR1
    • Bronchial hyperresponsiveness 1 (bronchial asthma)
    • IL 13
    • IL-13
    • Il13
    • IL13_HUMAN
    • Interleukin 13
    • Interleukin-13
    • interleukin13
    • MGC116786
    • MGC116788
    • MGC116789
    • NC 30
    • NC30
    • P 600
    • P600
    see all
  • Database links

    • Entrez Gene: 16163 Mouse
    • SwissProt: P20109 Mouse
    • Unigene: 1284 Mouse

    Associated products

    • ELISA kits

      • Mouse IL-13 Matched Antibody Pair Kit (ab221432)

    Images

    • Other - Mouse IL-13 ELISA Kit (ab219634)
      Other - Mouse IL-13 ELISA Kit (ab219634)

      SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

       

    • Example of mouse IL-13 standard curve.
      Example of mouse IL-13 standard curve.

      Background-subtracted data values (mean +/- SD) are graphed.

    • Interpolated concentrations of spike IL-13 in mouse serum and plasma samples.
      Interpolated concentrations of spike IL-13 in mouse serum and plasma samples.

      The concentrations of IL-13 were measured in duplicates, interpolated from the IL-13 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 10%, plasma (citrate) 10%, plasma (EDTA) 10% and plasma (heparin) 5%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).

    • Interpolated concentrations of native IL-13 in mouse serum and plasma samples.
      Interpolated concentrations of native IL-13 in mouse serum and plasma samples.

      The concentrations of IL-13 were measured in duplicates, interpolated from the IL-13 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 10%, plasma (citrate) 10%, plasma (EDTA) 10% and plasma (heparin) 5%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IL-13 concentration was determined to be 31.5 pg/mL in serum, 21.1 pg/mL in plasma (citrate) and 20.4 pg/mL in plasma (EDTA) and 29.1 pg/mL in plasma (heparin).

    • Interpolated concentrations of native IL-13 in mouse EL4/IL-2 cell culture supernatant unstimulated and PHA/PMA stimulated samples.
      Interpolated concentrations of native IL-13 in mouse EL4/IL-2 cell culture supernatant unstimulated and PHA/PMA stimulated samples.

      The concentrations of IL-13 were measured in duplicates, interpolated from the IL-13 standard curves and corrected for sample dilution. Undiluted samples are 2%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). T The mean IL-13 concentration was determined to be 3144 pg/mL in PHA/PMA stimulated EL4/IL-2 cell culture supernatant and undetermined in media and unstimulated cell culture supernatant.

    • Sandwich ELISA - Mouse IL-13 ELISA Kit (ab219634)
      Sandwich ELISA - Mouse IL-13 ELISA Kit (ab219634)
      To learn more about the advantages of recombinant antibodies see here.

    Protocols

    • Protocol Booklet

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
    • SDS
  • References (5)

    Publishing research using ab219634? Please let us know so that we can cite the reference in this datasheet.

    ab219634 has been referenced in 5 publications.

    • Pan Y  et al. Edaravone attenuates experimental asthma in mice through induction of HO-1 and the Keap1/Nrf2 pathway. Exp Ther Med 19:1407-1416 (2020). PubMed: 32010316
    • Wang YQ  et al. Gubenzhike Recipe Ameliorates Respiratory Mucosal Immunity in Mice with Chronic Obstructive Pulmonary Disease through Upregulation of the ?dT Lymphocytes and KGF Levels. Evid Based Complement Alternat Med 2020:3056797 (2020). PubMed: 32280354
    • Xu H  et al. Inhibition of TLR4 inhibits allergic responses in murine allergic rhinitis by regulating the NF-?B pathway. Exp Ther Med 18:761-768 (2019). PubMed: 31281453
    • Monga S  et al. Inhibitory Effects of the Two Novel TSPO Ligands 2-Cl-MGV-1 and MGV-1 on LPS-induced Microglial Activation. Cells 8:N/A (2019). PubMed: 31121852
    • Pang L  et al. Apigenin attenuates PM2.5-induced airway hyperresponsiveness and inflammation by down-regulating NF-?B in murine model of asthma. Int J Clin Exp Pathol 12:3700-3709 (2019). PubMed: 31933758

    Customer reviews and Q&As

    Show All Reviews Q&A
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    IL-13 ELISA kit

    Good Excellent 5/5 (Ease of Use)
    Abreviews
    Abreviews
    abreview image
    Good one. Easy to use and consume less time.

    Miss. Sheelu Monga

    Verified customer

    Submitted Jun 22 2018

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