SimpleStep

Mouse IL-13 ELISA Kit (ab219634)

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Overview

  • Product name

    Mouse IL-13 ELISA Kit
    See all IL-13 kits

  • Detection method

    Colorimetric

  • Precision

    Intra-assay
    Sample n Mean SD CV%
    supernatant 5 2.2%
    Inter-assay
    Sample n Mean SD CV%
    supernatant 3 1.4%

  • Sample type

    Cell culture supernatant, Serum, Hep Plasma, EDTA Plasma, Cit plasma

  • Assay type

    Sandwich (quantitative)

  • Sensitivity

    1.2 pg/ml

  • Range

    1.95 pg/ml - 125 pg/ml

  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 108 106% - 111%
    Serum 97 95% - 98%
    Hep Plasma 104 101% - 108%
    EDTA Plasma 98 97% - 99%
    Cit plasma 97 95% - 100%

  • Assay time

    1h 30m

  • Assay duration

    One step assay

  • Species reactivity

    Reacts with: Mouse

  • Product overview

    IL-13 in vitro SimpleStep ELISA® (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of IL-13 protein mouse in serum, plasma, and cell culture supernatant.


    The SimpleStep ELISA® employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material. TMB substrate is added and during incubation is catalyzed by HRP, generating blue coloration. This reaction is then stopped by addition of Stop Solution completing any color change from blue to yellow. Signal is generated proportionally to the amount of bound analyte and the intensity is measured at 450 nm. Optionally, instead of the endpoint reading, development of TMB can be recorded kinetically at 600 nm.


    Interleukin-13 (IL-13) is a secreted immunoregulatory cytokine in the IL-4/IL-13 family that is linked to allergic reactions and cancer pathogenesis. IL-13 inhibits inflammatory cytokine production and promotes immunoglobulin class switching on B-cells. IL-13 synergizes with IL2 in regulating interferon-gamma synthesis. IL-13 is linked to allergic rhinitis, a common disease of complex inheritance characterized by mucosal inflammation caused by allergen exposure.

  • Platform

    Microplate (12 x 8 well strips)

Properties

Images

  • Other - Mouse IL-13 ELISA Kit (ab219634)
    Other - Mouse IL-13 ELISA Kit (ab219634)

    SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

     

  • Example of mouse IL-13 standard curve.
    Example of mouse IL-13 standard curve.

    Background-subtracted data values (mean +/- SD) are graphed.

  • Interpolated concentrations of spike IL-13 in mouse serum and plasma samples.
    Interpolated concentrations of spike IL-13 in mouse serum and plasma samples.

    The concentrations of IL-13 were measured in duplicates, interpolated from the IL-13 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 10%, plasma (citrate) 10%, plasma (EDTA) 10% and plasma (heparin) 5%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).

  • Interpolated concentrations of native IL-13 in mouse serum and plasma samples.
    Interpolated concentrations of native IL-13 in mouse serum and plasma samples.

    The concentrations of IL-13 were measured in duplicates, interpolated from the IL-13 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 10%, plasma (citrate) 10%, plasma (EDTA) 10% and plasma (heparin) 5%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IL-13 concentration was determined to be 31.5 pg/mL in serum, 21.1 pg/mL in plasma (citrate) and 20.4 pg/mL in plasma (EDTA) and 29.1 pg/mL in plasma (heparin).

  • Interpolated concentrations of native IL-13 in mouse EL4/IL-2 cell culture supernatant unstimulated and PHA/PMA stimulated samples.
    Interpolated concentrations of native IL-13 in mouse EL4/IL-2 cell culture supernatant unstimulated and PHA/PMA stimulated samples.

    The concentrations of IL-13 were measured in duplicates, interpolated from the IL-13 standard curves and corrected for sample dilution. Undiluted samples are 2%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). T The mean IL-13 concentration was determined to be 3144 pg/mL in PHA/PMA stimulated EL4/IL-2 cell culture supernatant and undetermined in media and unstimulated cell culture supernatant.

Protocols

References

This product has been referenced in:

  • Xu H  et al. Inhibition of TLR4 inhibits allergic responses in murine allergic rhinitis by regulating the NF-?B pathway. Exp Ther Med 18:761-768 (2019). Read more (PubMed: 31281453) »
See 1 Publication for this product

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Customer reviews and Q&As

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IL-13 ELISA kit

 Good Excellent 5/5 (Ease of Use)
Abreviews
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Good one. Easy to use and consume less time.

Miss. Sheelu Monga

Verified customer

Submitted Jun 22 2018

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