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I ordered the IL-15 ELISA kit for abcam and i am running it today,
and i see that the amount of detection antibody included in the kit is not enough,
in assay method: step 4, asks for 100ul biotinylated antibody solution per well, for a 96 well plate is 9600ul total solution needed and actually 10000 ul will be the best,
in the preparation of reagents, in step 6, asks for 80 fold dilution of 100ul antibody which will result in only 8000 ul!
Asked on Dec 20 2012