Overview

  • Product name

    Mouse IL-17A ELISA Kit
    See all IL-17a kits
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Serum 5 4.9%
    Inter-assay
    Sample n Mean SD CV%
    Serum 3 10.6%
  • Sample type

    Cell culture supernatant, Serum, Citrate Plasma
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    0.5 pg/ml
  • Range

    6.25 pg/ml - 400 pg/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Serum 102.5 97.7% - 105.8%
    Cell culture media 91.1 82.7% - 99.7%
    Citrate Plasma 103.6 102.2% - 105.8%

  • Assay time

    1h 30m
  • Assay duration

    One step assay
  • Species reactivity

    Reacts with: Mouse
  • Product overview

    IL-17A mouse in vitro SimpleStep ELISA® (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of IL-17A protein in mouse serum, plasma (citrate) and cell culture supernatant samples.


    The SimpleStep ELISA® employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material. TMB substrate is added and during incubation is catalyzed by HRP, generating blue coloration. This reaction is then stopped by addition of Stop Solution completing any color change from blue to yellow. Signal is generated proportionally to the amount of bound analyte and the intensity is measured at 450 nm. Optionally, instead of the endpoint reading, development of TMB can be recorded kinetically at 600 nm.


    Sensitivity:


    Samples diluted in Sample Diluent NS – 0.5 pg/mL


    Samples diluted in Sample Diluent 25BP – 5.5 pg/mL

  • Notes

    IL-17A is a pro-inflammatory cytokine that is secreted by a subset of activated T cells. It is a disulfide-linked homodimer with both glycosylated and non-glycosylated forms. IL-17A induces stromal cells to produce pro-inflammatory and hematopoietic cytokines, and also enhances the surface expression of ICAM1/intracellular adhesion molecule 1 in fibroblasts.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Microplate (12 x 8 well strips)

Properties

  • Storage instructions

    Store at +4°C. Please refer to protocols.
  • Components 1 x 96 tests
    10X Mouse IL-17a Capture Antibody 1 x 600µl
    10X Mouse IL-17a Detector Antibody 1 x 600µl
    10X Wash Buffer PT (ab206977) 1 x 20ml
    Antibody Diluent CPI - HAMA Blocker (ab193969) 1 x 6ml
    Mouse IL-17a Lyophilized Recombinant Protein 2 vials
    Plate Seals 1 unit
    Sample Diluent 25BP 1 x 20ml
    Sample Diluent NS (ab193972) 1 x 50ml
    SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit
    Stop Solution 1 x 12ml
    TMB Development Solution 1 x 12ml
  • Research areas

  • Function

    Induces stromal cells to produce proinflammatory and hematopoietic cytokines. Enhances the surface expression of ICAM1/intracellular adhesion molecule 1 in fibroblasts.
  • Tissue specificity

    Restricted to activated memory T-cells.
  • Sequence similarities

    Belongs to the IL-17 family.
  • Post-translational
    modifications

    Found both in glycosylated and nonglycosylated forms.
  • Cellular localization

    Secreted.
  • Information by UniProt
  • Alternative names

    • CTLA8
    • IL17A
    • CTLA-8
    • Cytotoxic T-lymphocyte-associated antigen 8
    • IL-17
    • IL-17A
    • IL17
    • IL17_HUMAN
    • Il17a
    • Interleukin-17A
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab199081 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

     

  • Standard curve comparison between mouse IL-17A SimpleStep ELISA® kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows a 9-fold increase in sensitivity.

  • Background-subtracted data values (mean +/- SD) are graphed.

  • Background-subtracted data values (mean +/- SD) are graphed.

  • Titration of PHA+PMA stimulated EL4 cell culture supernatant within the working range of the assay. EL4 cells were cultured in the presence of 1.5% phytohemagglutinin (PHA) and 10 ng/mL phorbol 12-myristate 13-acetate (PMA) for 48 hours. The cell culture supernatant was collected and measured in 2-fold dilution series. Background-subtracted data values (mean +/- SD, n = 2) are graphed.

  • EL4 cells were cultured in the absence or presence of 1.5% PHA and 10 ng/mL PMA for 48 hours. The cell culture supernatants were collected and their dilutions (as indicated) were measured with this kit. Interpolated concentrations of IL-17a adjusted for sample dilution are graphed in ng of IL-17a per mL of supernatant (mean +/- SD, n = 2). Note that IL-17a is not detectable in the untreated EL4 supernatant samples.

Protocols

References

This product has been referenced in:

  • Jia CE  et al. Pendrin, an anion exchanger on lung epithelial cells, could be a novel target for lipopolysaccharide-induced acute lung injury mice. Am J Transl Res 8:981-92 (2016). Read more (PubMed: 27158384) »
See 1 Publication for this product

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