Overview

  • Product name

    Mouse IL-17C ELISA Kit
    See all IL-17C kits
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    1 16 1.3ng/ml 0.053 4.1%
    2 16 3.8ng/ml 0.186 4.9%
    3 16 5.7ng/ml 0.296 5.2%
    Inter-assay
    Sample n Mean SD CV%
    1 24 1.6ng/ml 0.086 5.4%
    2 24 4.5ng/ml 0.279 6.2%
    3 24 6.3ng/ml 0.422 6.7%
  • Sample type

    Cell culture supernatant, Serum, Cell Lysate, Heparin Plasma, EDTA Plasma
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    < 10 pg/ml
  • Range

    125 pg/ml - 8000 pg/ml
  • Assay time

    3h 30m
  • Assay duration

    Multiple steps standard assay
  • Species reactivity

    Reacts with: Mouse
  • Product overview

    The Mouse IL-17C Enzyme-Linked Immunosorbent Assay (ELISA) kit (ab213868) is designed for the quantitative measurement of Mouse IL-17C in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).


    The ELISA kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for IL-17C has been pre-coated onto 96-well plates. Standards (Expression system for standard: E.coli; Immunogen sequence: D17-Q194) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IL-17C is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB is used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Mouse IL-17C amount of sample captured in plate.

  • Notes

    IL-17C, also known as CX2, is a protein that in humans is encoded by the IL-17C gene. IL-17C is mapped to 16q24.3. The protein encoded by this gene is a T cell-derived cytokine that shares the sequence similarity with IL-17. This cytokine was reported to stimulate the release of tumor necrosis factor alpha and interleukin 1 beta from a monocytic cell line. The expression of this cytokine was found to be restricted to activated T cells. IL-17C is an essential autocrine cytokine regulating innate epithelial immune responses. It also plays an important role in the pathogenesis of inflammatory arthritis.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Pre-coated microplate (12 x 8 well strips)

Properties

Applications

Our Abpromise guarantee covers the use of ab213868 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • Mouse IL-17C ELISA Kit (ab213868) Standard Curve.

Protocols

References

ab213868 has not yet been referenced specifically in any publications.

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