Overview

  • Product name

    Mouse IL-17D ELISA Kit
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    1 16 715pg/ml 30.03 4.2%
    2 16 1410pg/ml 64.86 4.6%
    3 16 2703ng/ml 137.9 5.1%
    Inter-assay
    Sample n Mean SD CV%
    1 24 694pg/ml 38.17 5.5%
    2 24 1582pg/ml 99.67 6.3%
    3 24 2691pg/ml 180.3 6.7%
  • Sample type

    Cell culture supernatant, Serum, Cell Lysate, Heparin Plasma, EDTA Plasma, Tissue Homogenate
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    < 10 pg/ml
  • Range

    62.5 pg/ml - 4000 pg/ml
  • Assay time

    3h 30m
  • Assay duration

    Multiple steps standard assay
  • Species reactivity

    Reacts with: Mouse
  • Product overview

    The Mouse IL-17D Enzyme-Linked Immunosorbent Assay (ELISA) kit (ab213869) is designed for the quantitative measurement of Mouse IL-17D in cell culture supernatants, cell lysates, tissue homogenates, serum and plasma (heparin, EDTA).


    The ELISA kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for IL-17D has been pre-coated onto 96-well plates. Standards (Expression system for standard: E.coli; Immunogen sequence: A25-R205) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IL-17D is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB is used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Mouse IL-17D amount of sample captured in plate.

  • Notes

    IL-17D is a member of the unique IL-17 family, which share a conserved cysteine motif. It is mapped to 13q12.11. IL-17D regulates cytokine production in endothelial cells and inhibits hemopoiesis in vitro. Purified recombinant IL-17D inhibited myeloid progenitor cell colony formation. IL-17D can enhance endothelial cell production of IL6, IL8, and GMCSF, but not other cytokines, and it is preferentially expressed in skeletal muscle, brain, adipose tissue, heart, lung, and pancreas.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Pre-coated microplate (12 x 8 well strips)

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components Identifier 1 x 96 tests
    ABC Diluent Buffer Blue Cap 1 x 12ml
    Adhesive Plate Seal 4 units
    Antibody Diluent Buffer Green Cap 1 x 12ml
    Anti-Mouse IL-17D coated Microplate (12 x 8 wells) 1 unit
    Avidin-Biotin-Peroxidase Complex (ABC) 1 x 130µl
    Biotinylated anti- Mouse IL-17D antibody 1 x 130µl
    Lyophilized recombinant Mouse IL-17D standard 2 vials
    Sample Diluent Buffer Green Cap 1 x 30ml
    TMB Color Developing Agent Black Cap 1 x 10ml
    TMB Stop Solution Yellow Cap 1 x 10ml
  • Research areas

  • Function

    Induces expression of IL-6, IL-8, and GM-CSF from endothelial cells.
  • Tissue specificity

    Expressed preferentially in adipose, skeletal muscle and CNS.
  • Sequence similarities

    Belongs to the IL-17 family.
  • Cellular localization

    Secreted.
  • Information by UniProt
  • Alternative names

    • IL 17D
    • IL 22
    • IL 27
    • IL-17D
    • IL-27
    • IL17D
    • IL17D_HUMAN
    • IL27
    • Interleukin 17D
    • Interleukin 27
    • Interleukin-17D
    • Interleukin-27
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab213869 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • Mouse IL-17D ELISA Kit (ab213869) Standard Curve.

Protocols

References

ab213869 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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