Key features and details
- Sensitivity: 0.2 ng/ml
- Range: 0.2 ng/ml - 50 ng/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
Product nameMouse IL-17RB ELISA Kit
See all IL-17RB kits
Intra-assay Sample n Mean SD CV% Overall < 10% Inter-assay Sample n Mean SD CV% Overall < 12%
Sample typeCell culture supernatant, Serum, Plasma
Assay typeSandwich (quantitative)
Range0.2 ng/ml - 50 ng/ml
Sample specific recovery Sample type Average % Range Serum 84.11 69% - 98% Plasma 89.02 70% - 139% Cell culture media 74.63 70% - 80%
Assay durationMultiple steps standard assay
Species reactivityReacts with: Mouse
Mouse IL-17RB ELISA Kit is designed for the quantitative determination of IL-17RB in cell culture supernatants, plasma and serum samples.
Δ Note: Mouse IL-17RB concentration is low in normal serum/plasma and may not be detectable in this assay.
This assay employs an antibody specific for mouse IL-17RB coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-17RB present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse IL-17RB antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-17RB bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
This ELISA kit shows no cross-reactivity with the following cytokines tested: Mouse Adiponectin (ACRP30), ANGPTL1, ANGPTL3, Artemin, CCL28 (MEC), CD36 (SRB3), Chordin, CRP, E-Cadherin, Epigen, Epiregulin, Fas, Galectin-7, gp130, Granzyme B, Gremlin-1, IFN-gamma R1, IL-17B, IL-22, MIP-1beta, MMP-10, MMP-2, MMP-3, PDGF-AA, Persephin, sFRP-3, SLAM, Shh-N, TCK-1, TECK, TGF beta 1, Trance, TREML1, TWEAK, VEGF-B, VEGFR2.
PlatformPre-coated microplate (12 x 8 well strips)
Storage instructionsStore at -20°C. Please refer to protocols.
Components 1 x 96 tests 200X HRP-Streptavidin Concentrate 1 x 200µl 20X Wash Buffer 1 x 25ml 5X Assay Diluent B 1 x 15ml Anti-Mouse IL-17RB coated Microplate (12 x 8 wells) 1 unit Assay Diluent A 1 x 30ml Biotinylated Anti-Mouse IL-17RB Detection Antibody 2 vials Mouse IL-17RB Standard (Lyophilized) 2 vials Stop Solution 1 x 8ml TMB Substrate Solution 1 x 12ml
RelevanceRecently, a number of cytokines belonging to the interleukin IL-17 family have been identified. These are termed as IL-17B, IL-17C and IL-17E. IL-17 is a potent proinflammatory cytokine that plays roles in a number of diseases including rheumatoid arthritis, multiple sclerosis, and promotion of tumor growth. IL-17B, C, and E like IL-17 are able to induce proinflammatory responses. However, they do not bind to the IL-17 receptor suggesting that additional IL-17R related receptor might exist. Receptor for IL-17B and IL-17E have been independently isolated and have been designated as EV127 (in mouse) and IL-17RH1 (in human), respectively. IL-17E induces activation of NFkB pathway and like IL-17 also induces production of IL-8. IL-17RH1 mRNA is highly expressed in Kidney and liver and at moderate level in small intestine, colon, and brain.
Cellular localizationType I membrane protein (isoform 1). Secreted (isoform 2).
- CRL 4
- Cytokine receptor CRL4
ab267644 has not yet been referenced specifically in any publications.