Mouse IL-18 Matched Antibody Pair Kit (ab218808)
Key features and details
- Unlabeled capture antibody, biotin-labeled detection antibody and calibrated protein standard
- For economical ELISA and ELISA-based assay development
- Reacts with: Mouse
- Range: 31.25 pg/ml - 2000 pg/ml
Overview
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Product name
Mouse IL-18 Matched Antibody Pair Kit
See all IL-18 kits -
Detection method
Colorimetric -
Assay type
ELISA set -
Sensitivity
15.1 pg/ml -
Range
31.25 pg/ml - 2000 pg/ml -
Species reactivity
Reacts with: Mouse -
Product overview
Mouse IL-18 Matched Antibody Pair Kits include a capture and a biotinylated detector antibody pair, along with a calibrated protein standard, suitable for sandwich ELISA. The Matched Antibody Pair Kit can be used to quantify native and recombinant mouse IL-18.
Optimization of the kit reagents to sample type, immunoassay format or instrumentation may be required. Guidelines for use of this kit in a standard 96-well microplate sandwich ELISA using HRP/TMB system of colorimetric detection is described in this assay procedure for the purposes of quantification.
Protocol information and tips on the use of the Matched Antibody Pair kits for sandwich ELISA can be found on our website. An accessory pack can be purchased which includes buffer reagents required to perform 10 x 96-well plate sandwich ELISAs (ab210905).
For additional information on the performance of the antibody pair used in this kit, please see our equivalent SimpleStep ELISA kit ab216165. Please note that while the antibody pair is the same provided in the corresponding SimpleStep ELISA Kit, due to differences in their formulation, this antibody pair cannot be used with the consumables provided with our SimpleStep ELISA Kits.
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Tested applications
Suitable for: ELISAmore details -
Platform
Reagents
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 5 x 96 tests Mouse IL-18 Lyophilized Protein 1 vial Mouse IL-18 Capture Antibody 1 x 50µg Mouse IL-18 Detector Antibody 1 x 12.5µg -
Research areas
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Function
Augments natural killer cell activity in spleen cells and stimulates interferon gamma production in T-helper type I cells. -
Sequence similarities
Belongs to the IL-1 family. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- Iboctadekin
- IFN gamma inducing factor
- IFN-gamma-inducing factor
see all -
Database links
- Entrez Gene: 16173 Mouse
- SwissProt: P70380 Mouse
- Unigene: 1410 Mouse
Associated products
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Alternative Versions
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Related Products
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SimpleStep ELISA kits
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab218808 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ELISA |
Use at an assay dependent concentration.
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| Notes |
|---|
|
ELISA
Use at an assay dependent concentration. |
Images
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Mouse IL-18 standard curve
Standard calibration curve. Background subtracted values are graphed.
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Sandwich ELISA - Mouse IL-18 Matched Antibody Pair Kit (ab218808)To learn more about the advantages of recombinant antibodies see here.
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab218808 has been referenced in 2 publications.
- Wang D et al. YAP promotes the activation of NLRP3 inflammasome via blocking K27-linked polyubiquitination of NLRP3. Nat Commun 12:2674 (2021). PubMed: 33976226
- Chamoun-Emanuelli AM et al. NSAIDs disrupt intestinal homeostasis by suppressing macroautophagy in intestinal epithelial cells. Sci Rep 9:14534 (2019). PubMed: 31601922