Key features and details
- Unlabeled capture antibody, biotin-labeled detection antibody and calibrated protein standard
- For economical ELISA and ELISA-based assay development
- Reacts with: Mouse
- Range: 31.25 pg/ml - 2000 pg/ml
Product nameMouse IL-18 Matched Antibody Pair Kit
See all IL-18 kits
Assay typeELISA set
Range31.25 pg/ml - 2000 pg/ml
Species reactivityReacts with: Mouse
Mouse IL-18 Matched Antibody Pair Kits include a capture and a biotinylated detector antibody pair, along with a calibrated protein standard, suitable for sandwich ELISA. The Matched Antibody Pair Kit can be used to quantify native and recombinant mouse IL-18.
Optimization of the kit reagents to sample type, immunoassay format or instrumentation may be required. Guidelines for use of this kit in a standard 96-well microplate sandwich ELISA using HRP/TMB system of colorimetric detection is described in this assay procedure for the purposes of quantification.
Protocol information and tips on the use of the Matched Antibody Pair kits for sandwich ELISA can be found on our website. An accessory pack can be purchased which includes buffer reagents required to perform 10 x 96-well plate sandwich ELISAs (ab210905).
For additional information on the performance of the antibody pair used in this kit, please see our equivalent SimpleStep ELISA kit ab216165. Please note that while the antibody pair is the same provided in the corresponding SimpleStep ELISA Kit, due to differences in their formulation, this antibody pair cannot be used with the consumables provided with our SimpleStep ELISA Kits.
Tested applicationsSuitable for: ELISAmore details
Storage instructionsStore at -20°C. Please refer to protocols.
Components 10 x 96 tests 5 x 96 tests Mouse IL-18 Lyophilized Protein 2 vials 1 vial Mouse IL-18 Capture Antibody 2 x 50µg 1 x 50µg Mouse IL-18 Detector Antibody 2 x 12.5µg 1 x 12.5µg
FunctionAugments natural killer cell activity in spleen cells and stimulates interferon gamma production in T-helper type I cells.
Sequence similaritiesBelongs to the IL-1 family.
- Information by UniProt
- IFN gamma inducing factor
- IFN-gamma-inducing factor
SimpleStep ELISA kits
Our Abpromise guarantee covers the use of ab218808 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ELISA||Use at an assay dependent concentration.|
Standard calibration curve. Background subtracted values are graphed.
To learn more about the advantages of recombinant antibodies see here.
ab218808 has been referenced in 1 publication.
- Chamoun-Emanuelli AM et al. NSAIDs disrupt intestinal homeostasis by suppressing macroautophagy in intestinal epithelial cells. Sci Rep 9:14534 (2019). PubMed: 31601922