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  1. Link

    mouse-il-18-matched-antibody-pair-kit-ab218808.pdf

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Immunology Innate Immunity Cytokines Interleukins
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Recombinant

Mouse IL-18 Matched Antibody Pair Kit (ab218808)

  • Datasheet
  • SDS
  • Protocol Booklet
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Mouse IL-18 standard curve
  • Sandwich ELISA - Mouse IL-18 Matched Antibody Pair Kit (ab218808)

Key features and details

  • Unlabeled capture antibody, biotin-labeled detection antibody and calibrated protein standard
  • For economical ELISA and ELISA-based assay development
  • Reacts with: Mouse
  • Range: 31.25 pg/ml - 2000 pg/ml

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Overview

  • Product name

    Mouse IL-18 Matched Antibody Pair Kit
    See all IL-18 kits
  • Detection method

    Colorimetric
  • Assay type

    ELISA set
  • Sensitivity

    15.1 pg/ml
  • Range

    31.25 pg/ml - 2000 pg/ml
  • Species reactivity

    Reacts with: Mouse
  • Product overview

    Mouse IL-18 Matched Antibody Pair Kits include a capture and a biotinylated detector antibody pair, along with a calibrated protein standard, suitable for sandwich ELISA. The Matched Antibody Pair Kit can be used to quantify native and recombinant mouse IL-18.


    Optimization of the kit reagents to sample type, immunoassay format or instrumentation may be required. Guidelines for use of this kit in a standard 96-well microplate sandwich ELISA using HRP/TMB system of colorimetric detection is described in this assay procedure for the purposes of quantification.


    Protocol information and tips on the use of the Matched Antibody Pair kits for sandwich ELISA can be found on our website. An accessory pack can be purchased which includes buffer reagents required to perform 10 x 96-well plate sandwich ELISAs (ab210905).


    For additional information on the performance of the antibody pair used in this kit, please see our equivalent SimpleStep ELISA kit ab216165. Please note that while the antibody pair is the same provided in the corresponding SimpleStep ELISA Kit, due to differences in their formulation, this antibody pair cannot be used with the consumables provided with our SimpleStep ELISA Kits.

  • Tested applications

    Suitable for: ELISAmore details
  • Platform

    Reagents

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components 10 x 96 tests 5 x 96 tests
    Mouse IL-18 Lyophilized Protein 2 vials 1 vial
    Mouse IL-18 Capture Antibody 2 x 50µg 1 x 50µg
    Mouse IL-18 Detector Antibody 2 x 12.5µg 1 x 12.5µg
  • Research areas

    • Immunology
    • Innate Immunity
    • Cytokines
    • Interleukins
    • Cancer
    • Invasion/microenvironment
    • Angiogenesis
    • Angiogenic growth factors
    • Cancer
    • Tumor immunology
    • Cytokines
    • Interleukins
    • Cancer
    • Cancer Metabolism
    • Response to hypoxia
    • Metabolism
    • Pathways and Processes
    • Metabolism processes
    • Hypoxia
  • Function

    Augments natural killer cell activity in spleen cells and stimulates interferon gamma production in T-helper type I cells.
  • Sequence similarities

    Belongs to the IL-1 family.
  • Cellular localization

    Secreted.
  • Target information above from: UniProt accession Q14116 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Alternative names

    • Iboctadekin
    • IFN gamma inducing factor
    • IFN-gamma-inducing factor
    • IGIF
    • IL 1 gamma
    • IL 18
    • IL 1g
    • IL-1 gamma
    • IL-18
    • IL1 gamma
    • IL18
    • IL18 protein
    • IL18_HUMAN
    • IL1F4
    • IL1g
    • IL1gamma
    • ILIF4
    • Interferon gamma inducing factor
    • Interferon gamma-inducing factor
    • Interleukin 1 gamma
    • Interleukin 18
    • Interleukin 18 (interferon-gamma-inducing factor)
    • Interleukin-1 gamma
    • Interleukin-18
    • Interleukin18
    • MGC12320
    see all
  • Database links

    • Entrez Gene: 16173 Mouse
    • SwissProt: P70380 Mouse
    • Unigene: 1410 Mouse

    Associated products

    • Alternative Versions

      • Mouse IL-18 Antibody Pair - BSA and Azide free (ab241886)
    • Related Products

      • ELISA Accessory Pack (ab210905)
    • SimpleStep ELISA kits

      • Mouse IL-18 ELISA Kit (ab216165)

    Applications

    Our Abpromise guarantee covers the use of ab218808 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    ELISA Use at an assay dependent concentration.

    Images

    • Mouse IL-18 standard curve
      Mouse IL-18 standard curve

      Standard calibration curve. Background subtracted values are graphed.

    • Sandwich ELISA - Mouse IL-18 Matched Antibody Pair Kit (ab218808)
      Sandwich ELISA - Mouse IL-18 Matched Antibody Pair Kit (ab218808)
      To learn more about the advantages of recombinant antibodies see here.

    Protocols

    • Protocol Booklet

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
    • SDS
  • References (1)

    Publishing research using ab218808? Please let us know so that we can cite the reference in this datasheet.

    ab218808 has been referenced in 1 publication.

    • Chamoun-Emanuelli AM  et al. NSAIDs disrupt intestinal homeostasis by suppressing macroautophagy in intestinal epithelial cells. Sci Rep 9:14534 (2019). PubMed: 31601922

    Customer reviews and Q&As

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