Overview

  • Product name

    Mouse IL-18BP ELISA Kit
    See all IL-18BP kits
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Serum 8 3.7%
  • Sample type

    Serum, Citrate Plasma
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    151.82 pg/ml
  • Range

    468.75 pg/ml - 30000 pg/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Serum 97 % - %
    Citrate Plasma 98 % - %

  • Assay time

    1h 30m
  • Assay duration

    One step assay
  • Species reactivity

    Reacts with: Mouse
  • Product overview

    IL-18BP in vitro SimpleStep ELISA® (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of IL-18BP protein in mouse serum and plasma (citrate) samples


    The SimpleStep ELISA® employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material. TMB substrate is added and during incubation is catalyzed by HRP, generating blue coloration. This reaction is then stopped by addition of Stop Solution completing any color change from blue to yellow. Signal is generated proportionally to the amount of bound analyte and the intensity is measured at 450 nm. Optionally, instead of the endpoint reading, development of TMB can be recorded kinetically at 600 nm.


    IL-18BP is a protein that binds to IL-18, preventing it from interacting with its receptor. IL-18BP inhibits the early TH1 cytokine response. IL-18 is produced constitutively by mononuclear cells.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Pre-coated microplate (12 x 8 well strips)

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab254509 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

     

  • The IL-18BP standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

  • The concentrations of IL-18BP were measured in duplicates, interpolated from the IL-18BP standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 2.5% and plasma (citrate) 10%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IL-18BP concentration was determined to 175.87 ng/mL in serum and 201.01 ng/mL in plasma (citrate).

Protocols

References

ab254509 has not yet been referenced specifically in any publications.

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