Overview

  • Product name

    Mouse IL-2 ELISA Kit
    See all IL-2 kits
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Overall 5 4.7%
    Inter-assay
    Sample n Mean SD CV%
    Overall 3 7.6%
  • Sample type

    Cell culture supernatant, Serum, EDTA Plasma, Citrate Plasma
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    3.7 pg/ml
  • Range

    15.625 pg/ml - 1000 pg/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Serum 88 87% - 89%
    Cell culture media 117 115% - 120%
    EDTA Plasma 99 97% - 101%
    Citrate Plasma 94 90% - 98%

  • Assay time

    2h 30m
  • Assay duration

    Multiple steps standard assay
  • Species reactivity

    Reacts with: Mouse
    Does not react with: Rat, Human
  • Product overview

    IL-2 in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of IL-2 protein in mouse serum, plasma, and cell culture supernatant samples.


    The ELISA employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material. TMB substrate is added and during incubation is catalyzed by HRP, generating blue coloration. This reaction is then stopped by addition of Stop Solution completing any color change from blue to yellow. Signal is generated proportionally to the amount of bound analyte and the intensity is measured at 450 nm. Optionally, instead of the endpoint reading, development of TMB can be recorded kinetically at 600 nm.


    IL-2, also known as T cell growth factor (TCGF), is a glycosylated alpha-helical monomeric polypeptide. It is secreted by activated CD4+ and CD8+ T cells, neurons, microglia and hematopoietic stem cells in response to antigenic or mitogenic stimulation. IL-2 is required for T-cell proliferation, natural killer cells (NK) cytolytic activity, differentiation of regulatory T cells, modulation of T helper (Th) cell differentiation and activation-induced cell death. In particular, IL-2 modulates the expression of receptors for other cytokines and transcription factors, therefore regulating cytokine cascades that correlate with each of the Th differentiation states. Complete efficiency of IL-2 has been implicated in severe combined immunodeficiency, whereas reduction of IL-2 correlates with reduced function of CD4+CD25+ regulatory T cells and destabilization of immune homeostasis leading to autoimmune disease. Increased expression of IL-2 has also been implicated in inflammatory conditions such as inflammatory bowel disease and chronic liver diseases.


    Sensitivity:


    Samples diluted in Sample Diluent NS: 4.0 pg/mL.
    Samples diluted in Sample Diluent 10BS: 3.7 pg/mL.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Pre-coated microplate (12 x 8 well strips)

Properties

  • Storage instructions

    Store at +4°C. Please refer to protocols.
  • Components 1 x 96 tests
    10X Mouse IL-2 Capture Antibody 1 x 600µl
    10X Wash Buffer PT (ab206977) 2 x 20ml
    20X Mouse IL-2 Biotin Detector Antibody 20X 1 x 600µl
    Antibody Diluent 5BR 1 x 6ml
    Mouse IL-2 Lyophilized Recombinant Protein 2 vials
    Plate Seals 1 unit
    Sample Diluent 25BS 1 x 20ml
    Sample Diluent NS 1 x 50ml
    SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit
    Stop Solution 1 x 12ml
    Streptavidin-HRP 2 x 100µl
    TMB Development Solution 1 x 12ml
  • Research areas

  • Function

    Produced by T-cells in response to antigenic or mitogenic stimulation, this protein is required for T-cell proliferation and other activities crucial to regulation of the immune response. Can stimulate B-cells, monocytes, lymphokine-activated killer cells, natural killer cells, and glioma cells.
  • Involvement in disease

    Note=A chromosomal aberration involving IL2 is found in a form of T-cell acute lymphoblastic leukemia (T-ALL). Translocation t(4;16)(q26;p13) with involves TNFRSF17.
  • Sequence similarities

    Belongs to the IL-2 family.
  • Cellular localization

    Secreted.
  • Information by UniProt
  • Alternative names

    • Aldesleukin
    • IL 2
    • IL-2
    • IL2
    • IL2_HUMAN
    • Interleukin 2
    • Interleukin-2
    • interleukin2
    • Involved in regulation of T cell clonal expansion
    • Lymphokine
    • OTTHUMP00000164090
    • POIL2
    • T Cell Growth Factor
    • T-cell growth factor
    • TCGF
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab223588 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • Standard curve comparison between Mouse IL-2 SimpleStep ELISA® kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows comparable sensitivity.

  • Background-subtracted data values (mean +/- SD) are graphed.

  • Background-subtracted data values (mean +/- SD) are graphed.

  • The concentrations of IL-2 were measured in duplicates, interpolated from the IL-2 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum neat, plasma (citrate) neat, and plasma (EDTA) neat. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).

  • The concentrations of IL-2 were measured in duplicates, interpolated from the IL-2 standard curves and corrected for sample dilution. Undiluted samples are as follows: stimulated EL4.IL2 cell culture supernatant 2%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IL-2 concentration was determined to be 47.25 ng/mL in neat PHA+PMA stimulated EL4.IL2 cell culture supernatant.

  • EL4.IL2 cells were cultured for 2 days in the presence or absence of 10 µg/mL PHA and 10 ng/mL PMA. The concentrations of IL-2 were measured in three different dilutions of the supernatant samples in duplicates and interpolated from the IL-2 standard curve. The interpolated values are plotted (mean +/- SD, n=3) in ng per mL of neat supernatant. The mean IL-2 concentration was determined to be 47.25 ng/mL in PHA+PMA stimulated EL4.IL2 cell culture supernatant and undetectable in unstimulated EL4.IL2 cell culture supernatant and DMEM media (not shown).

Protocols

References

ab223588 has not yet been referenced specifically in any publications.

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