Mouse IL-20 ELISA Kit (ab235645)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 7 pg/ml
- Range: 15.6 pg/ml - 1000 pg/ml
- Sample type: Cell culture supernatant, Cit plasma, EDTA Plasma, Hep Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
Overview
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Product name
Mouse IL-20 ELISA Kit
See all IL-20 kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Serum 5 3.1% Inter-assay Sample n Mean SD CV% Serum 3 7.4% -
Sample type
Cell culture supernatant, Serum, Hep Plasma, EDTA Plasma, Cit plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
7 pg/ml -
Range
15.6 pg/ml - 1000 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 105 95% - 120% Cell culture media 101 92% - 111% Hep Plasma 99 91% - 114% EDTA Plasma 88 79% - 103% Cit plasma 108 99% - 115% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Mouse -
Product overview
Mouse IL-20 ELISA Kit (ab235645) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of IL-20 protein in cell culture supernatant, cit plasma, edta plasma, hep plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse IL-20 with 7 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Mouse IL-20 Capture Antibody 1 x 600µl 10X Mouse IL-20 Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml Antibody Diluent 4BR 1 x 6ml Mouse IL-20 Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
Cytokine that may be involved in epidermal function and psoriasis. Acts through STAT3. -
Tissue specificity
Expressed at very low levels in skin, trachea, and other tissues. -
Sequence similarities
Belongs to the IL-10 family. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- Cytokine Zcyto10
- Four alpha helix cytokine
- IL-20
see all -
Database links
- Entrez Gene: 58181 Mouse
- SwissProt: Q9JKV9 Mouse
- Unigene: 103794 Mouse
Associated products
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Alternative Versions
Images
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Other - Mouse IL-20 ELISA Kit (ab235645)SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Mouse IL-20 Standard Curve ComparisonStandard Curve comparison between Mouse IL-20 SimpleStep ELISA kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows comparable sensitivity.
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Interpolated concentrations of native IL-20 in mouse serum and plasma samples.The concentrations of IL-20 were measured in duplicates, interpolated from the IL-20 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 50%, plasma (EDTA) 50%, plasma (citrate) 50% and plasma (heparin) 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IL-20 concentration was determined to be 221 pg/mL in serum, 212 pg/mL in plasma (EDTA), 207 pg/mL in plasma (citrate), and 206 pg/mL in plasma (heparin).
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Interpolated concentrations of spike IL-20 in Mouse cell culture media sample.The concentrations of IL-20 were measured in duplicate and interpolated from the IL-20 standard curve and corrected for sample dilution.
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Example of mouse IL-20 standard curve in Sample Diluent NS.Background-subtracted data values (mean +/- SD) are graphed.
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Sandwich ELISA - Mouse IL-20 ELISA Kit (ab235645)To learn more about the advantages of recombinant antibodies see here.
References (0)
ab235645 has not yet been referenced specifically in any publications.
