Mouse IL-20 ELISA Kit (ab235645)
- Datasheet
- References
- Protocols
Overview
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Product name
Mouse IL-20 ELISA Kit
See all IL-20 kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Serum 5 3.1% Inter-assay Sample n Mean SD CV% Serum 3 7.4% -
Sample type
Cell culture supernatant, Serum, Hep Plasma, EDTA Plasma, Cit plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
7 pg/ml -
Range
15.6 pg/ml - 1000 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 105 95% - 120% Cell culture media 101 92% - 111% Hep Plasma 99 91% - 114% EDTA Plasma 88 79% - 103% Cit plasma 108 99% - 115% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Mouse -
Product overview
IL-20 in vitro SimpleStep ELISA® (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of IL-20 protein in mouse serum, plasma and cell culture media.
The SimpleStep ELISA® employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material. TMB Development Solution is added and during incubation is catalyzed by HRP, generating blue coloration. This reaction is then stopped by addition of Stop Solution completing any color change from blue to yellow. Signal is generated proportionally to the amount of bound analyte and the intensity is measured at 450 nm. Optionally, instead of the endpoint reading, development of TMB can be recorded kinetically at 600 nm.
IL-20 is a cytokine structurally related to IL-10. IL-20 is expressed in monocytes and keratinocytes and is upregulated during inflammation. IL-20 binds to IL-20R2 and IL-20R1 receptors forming complexes that can result in hyperproliferation of keratinocytes, and cause the onset of other inflammatory disease such as rheumatic arthritis, psoriasis, and atherosclerosis.
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Tested applications
Suitable for: Sandwich ELISAmore details -
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Mouse IL-20 Capture Antibody 1 x 600µl 10X Mouse IL-20 Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml Antibody Diluent 4BR 1 x 6ml Mouse IL-20 Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
Cytokine that may be involved in epidermal function and psoriasis. Acts through STAT3. -
Tissue specificity
Expressed at very low levels in skin, trachea, and other tissues. -
Sequence similarities
Belongs to the IL-10 family. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- Cytokine Zcyto10
- Four alpha helix cytokine
- IL-20
see all -
Database links
- Entrez Gene: 58181 Mouse
- SwissProt: Q9JKV9 Mouse
- Unigene: 103794 Mouse
Associated products
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Alternative Versions
Applications
Our Abpromise guarantee covers the use of ab235645 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Sandwich ELISA | Use at an assay dependent concentration. |
Images
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Other - Mouse IL-20 ELISA Kit (ab235645)SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Mouse IL-20 Standard Curve ComparisonStandard Curve comparison between Mouse IL-20 SimpleStep ELISA kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows comparable sensitivity.
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Interpolated concentrations of native IL-20 in mouse serum and plasma samples.The concentrations of IL-20 were measured in duplicates, interpolated from the IL-20 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 50%, plasma (EDTA) 50%, plasma (citrate) 50% and plasma (heparin) 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IL-20 concentration was determined to be 221 pg/mL in serum, 212 pg/mL in plasma (EDTA), 207 pg/mL in plasma (citrate), and 206 pg/mL in plasma (heparin).
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Interpolated concentrations of spike IL-20 in Mouse cell culture media sample.The concentrations of IL-20 were measured in duplicate and interpolated from the IL-20 standard curve and corrected for sample dilution.
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Example of mouse IL-20 standard curve in Sample Diluent NS.Background-subtracted data values (mean +/- SD) are graphed.
Protocols
References
ab235645 has not yet been referenced specifically in any publications.
