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    mouse-il-21-elisa-kit-ab100707.pdf

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Immunology Innate Immunity Cytokines Interleukins
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Mouse IL-21 ELISA Kit (ab100707)

  • Datasheet
  • SDS
  • Protocol Booklet
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Typical standard curve
  • Typical standard curve

Key features and details

  • Sensitivity: 6 pg/ml
  • Range: 8.19 pg/ml - 2000 pg/ml
  • Sample type: Cell culture supernatant, Plasma
  • Detection method: Colorimetric
  • Assay type: Sandwich (quantitative)
  • Reacts with: Mouse

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Protein
Product image
Recombinant mouse IL-21 protein (Active) (ab256054)

View more associated products

Overview

  • Product name

    Mouse IL-21 ELISA Kit
    See all IL-21 kits
  • Detection method

    Colorimetric
  • Sample type

    Cell culture supernatant, Plasma
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    < 6 pg/ml
  • Range

    8.19 pg/ml - 2000 pg/ml
  • Recovery

    89 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 98.65 87% - 109%
    Plasma 77.38 68% - 87%
  • Assay duration

    Multiple steps standard assay
  • Species reactivity

    Reacts with: Mouse
  • Product overview

    Abcam’s IL-21 Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse IL-21 in plasma and cell culture supernatants. (Mouse IL-21 concentration is pretty low in normal plasma, it may not be detected in this assay).


    This assay employs an antibody specific for mouse IL-21 coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-21 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse IL-21 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-21 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.


    We have not been able to detect endogenous Mouse IL-21 in normal serum with ab100707, only in serum spiked with Mouse IL-21.

  • Platform

    Microplate

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components 1 x 96 tests
    200X HRP-Streptavidin Concentrate 1 x 200µl
    20X Wash Buffer Concentrate 1 x 25ml
    5X Assay Diluent B 1 x 15ml
    Assay Diluent A 1 x 30ml
    Biotinylated anti-mouse IL-21 2 vials
    IL-21 Microplate (12 x 8 wells) 1 unit
    Recombinant mouse IL-21 Standard (lyophilized) 2 vials
    Stop Solution 1 x 8ml
    TMB One-Step Substrate Reagent 1 x 12ml
  • Research areas

    • Immunology
    • Innate Immunity
    • Cytokines
    • Interleukins
    • Cancer
    • Tumor immunology
    • Cytokines
    • Interleukins
    • Kits/ Lysates/ Other
    • Kits
    • ELISA Kits
    • ELISA Kits
    • Interleukin ELISA kits
    • Kits/ Lysates/ Other
    • Kits
    • ELISA Kits
    • ELISA Kits
    • Cytokines and cytokine receptors ELISA kits
  • Relevance

    A novel cytokine related to IL-2 and IL-15 was recently identified and designated IL-21. IL-21 has been found to be a powerful growth factor for naive B cells. The receptor for IL-21 (IL-21R, also termed NILR for novel Interleukin receptor) is a new member of the class I cytokine receptor family. IL-21R forms a complex with the common cytokine receptor g chain, gc, and mediates IL-21 signaling. Both IL-21R and the gc are necessary for the IL-21 function. IL-21 and its receptor activate JAKSTAT signaling pathway. IL-21R is expressed in spleen, thymus, natural killer (NK), T and B cell lines. IL-21 plays a role in the proliferation and maturation of NK, B and T cell populations.
  • Cellular localization

    Secreted
  • Alternative names

    • Cytokine CX2
    • DN 452A22.7
    • IL 21
    • IL21
    • Interleukin 21
    • Interleukin 21 isoform
    • Interleukin21
    • OTTMUSP00000007838
    • Za11
    see all
  • Database links

    • Entrez Gene: 60505 Mouse
    • SwissProt: Q9ES17 Mouse

    Associated products

      Images

      • Typical standard curve
        Typical standard curve

        Representative standard curve using ab100707

      • Typical standard curve
        Typical standard curve

        Representative standard curve using ab100707

      Protocols

      • Protocol Booklet

      Click here to view the general protocols

      Datasheets and documents

      • SDS download

      • Datasheet download

        Download

      References (0)

      Publishing research using ab100707? Please let us know so that we can cite the reference in this datasheet.

      ab100707 has not yet been referenced specifically in any publications.

      Customer reviews and Q&As

      Show All Reviews Q&A
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      1-2 of 2 Abreviews or Q&A

      Question

      starting dilution for plasma samples?

      Read More

      Abcam community

      Verified customer

      Asked on Apr 02 2013

      Answer


      We recommend a 2 fold dilution for plasma samples for use with all three of the mouse ELISA kits.

      Read More

      Abcam Scientific Support

      Answered on Apr 02 2013

      Question

      Product code: 100707
      Inquiry: Hi, I am interested in your mouse IL-21 ELISA kit. I would like to konw if the antibodies provided with the kit are polyclonal or monoclonal. Hope you can help, and thanks.

      Read More

      Abcam community

      Verified customer

      Asked on May 02 2012

      Answer

      Thank you for your enquiry.

      I can confirm that both the antibodies provided with ab100707 IL21 Mouse ELISA Kit are polyclonal.

      I hope this will be helpful. If you have any further questions, please do not hesitate to contact us.

      Read More

      Abcam Scientific Support

      Answered on May 02 2012

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
      For licensing inquiries, please contact partnerships@abcam.com

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