Mouse IL-21 ELISA Kit (ab100707)
- Datasheet
- References
- Protocols
Overview
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Product name
Mouse IL-21 ELISA Kit
See all IL-21 kits -
Detection method
Colorimetric -
Sample type
Cell culture supernatant, Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
< 6 pg/ml -
Range
8.19 pg/ml - 2000 pg/ml -
Recovery
89 %
Sample specific recovery Sample type Average % Range Cell culture supernatant 98.65 87% - 109% Plasma 77.38 68% - 87% -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Mouse -
Product overview
Abcam’s IL-21 Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse IL-21 in plasma and cell culture supernatants. (Mouse IL-21 concentration is pretty low in normal plasma, it may not be detected in this assay).
This assay employs an antibody specific for mouse IL-21 coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-21 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse IL-21 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-21 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
We have not been able to detect endogenous Mouse IL-21 in normal serum with ab100707, only in serum spiked with Mouse IL-21.
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Platform
Microplate
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 200X HRP-Streptavidin Concentrate 1 x 200µl 20X Wash Buffer Concentrate 1 x 25ml 5X Assay Diluent B 1 x 15ml Assay Diluent A 1 x 30ml Biotinylated anti-mouse IL-21 2 vials IL-21 Microplate (12 x 8 wells) 1 unit Recombinant mouse IL-21 Standard (lyophilized) 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml -
Research areas
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Relevance
A novel cytokine related to IL-2 and IL-15 was recently identified and designated IL-21. IL-21 has been found to be a powerful growth factor for naive B cells. The receptor for IL-21 (IL-21R, also termed NILR for novel Interleukin receptor) is a new member of the class I cytokine receptor family. IL-21R forms a complex with the common cytokine receptor g chain, gc, and mediates IL-21 signaling. Both IL-21R and the gc are necessary for the IL-21 function. IL-21 and its receptor activate JAKSTAT signaling pathway. IL-21R is expressed in spleen, thymus, natural killer (NK), T and B cell lines. IL-21 plays a role in the proliferation and maturation of NK, B and T cell populations. -
Cellular localization
Secreted -
Alternative names
- Cytokine CX2
- DN 452A22.7
- IL 21
see all -
Database links
- Entrez Gene: 60505 Mouse
- SwissProt: Q9ES17 Mouse
Images
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Typical standard curveRepresentative standard curve using ab100707
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Typical standard curveRepresentative standard curve using ab100707
Protocols
References
ab100707 has not yet been referenced specifically in any publications.
