Overview

  • Product name

    Mouse IL-22 ELISA Kit
    See all IL-22 kits
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Serum 9 3.8%
    Inter-assay
    Sample n Mean SD CV%
    Serum 3 6.2%
  • Sample type

    Cell culture supernatant, Serum, Plasma, Tissue Extracts, EDTA Plasma, Citrate Plasma
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    0.15 pg/ml
  • Range

    3.13 pg/ml - 200 pg/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 107 104% - 110%
    Serum 99 98% - 101%
    Plasma 101 104% - 110%
    EDTA Plasma 100 98% - 101%
    Citrate Plasma 95 94% - 96%

  • Assay time

    1h 30m
  • Assay duration

    One step assay
  • Species reactivity

    Reacts with: Mouse
    Does not react with: Rabbit, Cow, Human
  • Product overview

    IL-22 in vitro SimpleStep ELISA® (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of IL-22 protein in Mouse plasma, serum, and cell culture supernatants.


    The SimpleStep ELISA® employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material. TMB substrate is added and during incubation is catalyzed by HRP, generating blue coloration. This reaction is then stopped by addition of Stop Solution completing any color change from blue to yellow. Signal is generated proportionally to the amount of bound analyte and the intensity is measured at 450 nm. Optionally, instead of the endpoint reading, development of TMB can be recorded kinetically at 600 nm.


    Interleukin 22 (IL-22) is a cytokine thought to be involved in the response to inflammation through the Jak/STAT and MAPK signaling pathways. IL-22 is produced by NK cells and Th1-type T cells and can stimulate the induction of pro-inflammatory cytokines in human keratinocytes.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Pre-coated microplate (12 x 8 well strips)

Properties

  • Storage instructions

    Store at +4°C. Please refer to protocols.
  • Components 1 x 96 tests
    10X Wash Buffer PT (ab206977) 1 x 12ml
    Antibody Diluent 4BR 1 x 6ml
    10X Mouse IL-22 Capture Antibody 1 x 600µl
    10X Mouse IL-22 Detector Antibody 1 x 600ml
    Mouse IL-22 Lyophilized Recombinant Protein 2 vials
    Plate Seals 1 unit
    Sample Diluent NS 1 x 50ml
    SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit
    Stop Solution 1 x 12ml
    TMB Development Solution 1 x 12ml
  • Research areas

  • Function

    Cytokine that contributes to the inflammatory response in vivo.
  • Sequence similarities

    Belongs to the IL-10 family.
  • Cellular localization

    Secreted.
  • Information by UniProt
  • Alternative names

    • Cytokine Zcyto18
    • IL 10 related T cell derived inducible factor
    • IL 21
    • IL 22
    • IL D110
    • IL TIF
    • IL-10-related T-cell-derived-inducible factor
    • IL-22
    • IL-TIF
    • IL21
    • Il22
    • IL22_HUMAN
    • ILD110
    • ILTIF
    • Interleukin 10 related T cell derived inducible factor
    • interleukin 21
    • Interleukin 22
    • Interleukin-22
    • MGC79382
    • MGC79384
    • TIFa
    • TIFIL 23
    • TIFIL23
    • UNQ3099/PRO10096
    • zcyto18
    see all
  • Database links

Associated products

Applications

Our Abpromise guarantee covers the use of ab223857 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

     

  • Standard curve comparison between mouse IL-22 SimpleStep ELISA® kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows comparable sensitivity.

  • Background-subtracted data values (mean +/- SD) are graphed.

  • The concentrations of IL-22 were measured in duplicates, interpolated from the IL-22 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 25%, plasma (citrate) 50%, plasma (heparin) 50% and plasma (EDTA) 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IL-22 concentration was determined to be 117 pg/mL in serum, 101 pg/mL in plasma (citrate), 193 pg/mL in plasma (heparin), and 137 pg/mL in plasma (EDTA).

  • The concentrations of IL-22 were measured in duplicate and interpolated from the IL-22 standard curve and corrected for sample dilution. Undiluted samples are as follows: EL4.IL2 supernatant 25% and spleen supernatant 25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IL-22 concentration was determined to be 500 pg/mL in EL4.IL2 supernatant and 225 pg/mL in spleen supernatant.

  • EL4.IL2 cells were cultured to semi-confluency and then treated for 48 hours with either 10 µg/mL PHA + 10 ng/mL PMA or DMSO (mock). Rat spleen cells were cultured in the presence or absence of 100 ng/mL IFN-gamma + 1 µg/mL LPS for 4 days. The concentrations of IL-22 were measured in five different dilutions of the supernatant samples in duplicates and interpolated from the IL-22 standard curve. The interpolated values are plotted (mean +/- SD, n=5) in pg per mL of neat supernatant. The mean IL-22 concentration was determined to be 500 pg/mL in stimulated EL4.IL2 supernatant and undetectable in unstimulated EL4.IL2 supernatant. The mean concentration was determined to be 225 pg/mL in stimulated spleen supernatant and 1.7 pg/mL in unstimulated spleen supernatant.

Protocols

References

ab223857 has not yet been referenced specifically in any publications.

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