Overview

  • Product name

    Mouse IL-28 ELISA Kit
  • Detection method

    Colorimetric
  • Sample type

    Cell culture supernatant, Serum, Plasma
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    < 2 pg/ml
  • Range

    2.46 pg/ml - 600 pg/ml
  • Recovery

    88 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 74.3 67% - 87%
    Serum 109.8 98% - 118%
    Plasma 76.8 67% - 93%

  • Assay duration

    Multiple steps standard assay
  • Species reactivity

    Reacts with: Mouse
  • Product overview

    Abcam’s IL-28 Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Mouse IL-28 in serum, plasma and cell culture supernatants.

    This assay employs an antibody specific for IL-28 coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-28 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Mouse IL-28 antibody is added. After washing away unbound biotinylated antibody, HRP conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-28 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Microplate

Properties

Applications

Our Abpromise guarantee covers the use of ab100708 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • Representative standard curve using ab100708

Protocols

References

This product has been referenced in:

  • Okamoto M  et al. IPS-1 is essential for type III IFN production by hepatocytes and dendritic cells in response to hepatitis C virus infection. J Immunol 192:2770-7 (2014). Read more (PubMed: 24532585) »
See 1 Publication for this product

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