Overview

  • Product name

    Mouse IL-28A ELISA Kit
    See all IL-28A kits
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Serum 5 6.7%
    Inter-assay
    Sample n Mean SD CV%
    Serum 3 4.1%
  • Sample type

    Cell culture supernatant, Serum, EDTA Plasma, Citrate Plasma
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    2.4 pg/ml
  • Range

    15.63 pg/ml - 1000 pg/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 105 102% - 107%
    Serum 111 110% - 112%
    Cell culture media 108 106% - 108%
    EDTA Plasma 106 106% - 106%
    Citrate Plasma 111 109% - 112%

  • Assay time

    1h 30m
  • Assay duration

    One step assay
  • Species reactivity

    Reacts with: Mouse
  • Product overview

    IL-28A (Interleukin-28A) in vitro SimpleStep ELISA® (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of IL-28A protein in serum, plasma, and cell culture supernatants.


    The SimpleStep ELISA® employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material.  TMB substrate is added and during incubation is catalyzed by HRP, generating blue coloration. This reaction is then stopped by addition of Stop Solution completing any color change from blue to yellow. Signal is generated proportionally to the amount of bound analyte and the intensity is measured at 450 nm. Optionally, instead of the endpoint reading, development of TMB can be recorded kinetically at 600 nm.

  • Notes

    IL-28A (Interleukin 28A), also known as IFN-lambda 2 (Interferon-lambda 2), is a class II cytokine receptor ligand with antiviral, antitumor, and immunomodulatory activities.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Microplate (12 x 8 well strips)

Properties

  • Storage instructions

    Store at +4°C. Please refer to protocols.
  • Components 1 x 96 tests
    10X Mouse IL-28A Capture Antibody 1 x 600µl
    10X Mouse IL-28A Detector Antibody 1 x 600µl
    10X Wash Buffer PT (ab206977) 1 x 20ml
    Antibody Diluent 5BI 1 x 6ml
    Mouse IL-28A Lyophilized Recombinant Protein 2 vials
    Plate Seals 1 unit
    Sample Diluent NS (ab193972) 1 x 50ml
    SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit
    Stop Solution 1 x 12ml
    TMB Development Solution 1 x 12ml
  • Research areas

  • Function

    Cytokine with immunomodulatory activity. Up-regulates MHC class I antigen expression. Displays potent antiviral activity. Also displays antitumor activity. Ligand for the heterodimeric class II cytokine receptor composed of IL10RB and IL28RA. The ligand/receptor complex seems to signal through the Jak-STAT pathway.
  • Sequence similarities

    Belongs to the IL-28/IL-29 family.
  • Cellular localization

    Secreted.
  • Information by UniProt
  • Alternative names

    • Cytokine ZCYTO20
    • IFN-lambda-2
    • IFNL2
    • IL-28A
    • IL28A
    • IL28A_HUMAN
    • Interferon lambda 2
    • Interferon lambda-2
    • Interleukin 28A
    • interleukin 28A (interferon, lambda 2)
    • Interleukin-28A
    • ZCYTO20
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab208989 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

     

  • Background-subtracted data values (mean +/- SD) are graphed.

  • The concentrations of IL-28A were measured in duplicates, interpolated from the (target) standard curves and corrected for sample dilution. 1X diluted samples are as follows:  serum 12.5% and plasma (citrate) 12.5%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).

  • The concentrations of IL-28A were measured in duplicates, interpolated from the (target) standard curves and corrected for sample dilution. 1X diluted samples are as follows:  lung cell culture supernatant 25%, and RAW 264.7 cell culture supernatant 25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).

Protocols

References

ab208989 has not yet been referenced specifically in any publications.

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