Mouse IL-31 ELISA Kit (ab213872)
Key features and details
- Sensitivity: 10 pg/ml
- Range: 62.5 pg/ml - 4000 pg/ml
- Sample type: Cell culture supernatant, Cell Lysate, EDTA Plasma, Hep Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
Overview
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Product name
Mouse IL-31 ELISA Kit
See all IL-31 kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% 1 16 387pg/ml 21.3 5.5% 2 16 1228pg/ml 78.6 6.4% 3 16 2155pg/ml 99.13 4.6% Inter-assay Sample n Mean SD CV% 1 24 453pg/ml 31.26 6.9% 2 24 1396pg/ml 100.5 7.2% 3 24 2370pg/ml 128 5.4% -
Sample type
Cell culture supernatant, Serum, Cell Lysate, Hep Plasma, EDTA Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
< 10 pg/ml -
Range
62.5 pg/ml - 4000 pg/ml -
Assay time
3h 30m -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Mouse -
Product overview
The Mouse IL-31 Enzyme-Linked Immunosorbent Assay (ELISA) kit (ab213872) is designed for the quantitative measurement of Mouse IL-31 in cell culture supernatants, cell lysates, tissue homogenates, serum and plasma (heparin, EDTA).
The ELISA kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for IL-31 has been pre-coated onto 96-well plates. Standards (Expression system for standard: E.coli; Immunogen sequence: T24-C163) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IL-31 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB is used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Mouse IL-31 amount of sample captured in plate.
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Notes
Interleukin-31, also known as IL-31 is a protein that in humans is encoded by the IL31 gene. The IL-31 gene is mapped to chromosome 12q24.31 and the mouse gene maps to a syntenic region on chromosome 5. IL-31, which is made principally by activated Th2-type T cells, interacts with a heterodimeric receptor consisting of IL-31RA and OSMR that is constitutively expressed on epithelial cells and keratinocytes. IL-31 may be involved in the promotion of allergic skin disorders and in regulating other allergic diseases, such as asthma.
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components Identifier 1 x 96 tests 1 x 96 tests ABC Diluent Buffer Blue Cap 1 x 12ml 1 x 12ml Adhesive Plate Seal 4 units 4 units Antibody Diluent Buffer Green Cap 1 x 12ml 1 x 12ml Anti-Mouse IL-31 coated Microplate (12 x 8 wells) 1 unit 1 unit Avidin-Biotin-Peroxidase Complex (ABC) 1 x 100µl 1 x 100µl Biotinylated anti- Mouse IL-31 antibody 1 x 100µl 1 x 100µl Lyophilized recombinant Mouse IL-31 standard 2 vials 2 vials Sample Diluent Buffer Green Cap 1 x 30ml 1 x 30ml TMB Color Developing Agent Black Cap 1 x 10ml 1 x 10ml TMB Stop Solution Yellow Cap 1 x 10ml 1 x 10ml Wash Buffer (25X) 1 x 20ml 1 x 20ml -
Research areas
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Function
Activates STAT3 and possibly STAT1 and STAT5 through the IL31 heterodimeric receptor composed of IL31RA and OSMR. IL31 may function in skin immunity. -
Tissue specificity
Detected at low levels in testis, bone marrow, skeletal muscle, kidney, colon, thymus, small intestine and trachea. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- IL 31
- IL-31
- IL31
see all -
Database links
- Entrez Gene: 76399 Mouse
- SwissProt: Q6EAL8 Mouse
- Unigene: 75049 Mouse
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab213872 has not yet been referenced specifically in any publications.