Key features and details
- Sensitivity: 1 pg/ml
- Range: 2.05 pg/ml - 500 pg/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
Product nameMouse IL-5 ELISA Kit
See all IL-5 kits
Sample typeCell culture supernatant, Serum, Plasma
Assay typeSandwich (quantitative)
Sensitivity< 1 pg/ml
Range2.05 pg/ml - 500 pg/ml
Sample specific recovery Sample type Average % Range Cell culture supernatant 95.76 85% - 104% Serum 93.44 82% - 102% Plasma 95.49 93% - 104%
Assay durationMultiple steps standard assay
Species reactivityReacts with: Mouse
Abcam’s IL-5 Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse IL-5 in serum, plasma and cell culture supernatants.
This assay employs an antibody specific for mouse IL-5 coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-5 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse IL-5 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-5 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Get better reproducibility in only 90 minutes with Mouse IL-5 ELISA Kit (ab204523) from our SimpleStep ELISA® range.
Storage instructionsStore at -20°C. Please refer to protocols.
Components 1 x 96 tests 20X Wash Buffer Concentrate 1 x 25ml 400X HRP-Streptavidin Concentrate 1 x 200µl 5X Assay Diluent B 1 x 15ml Assay Diluent A 1 x 30ml Biotinylated anti-mouse IL-5 (lyophilized) 2 vials IL-5 Microplate (12 x 8 wells) 1 unit Recombinant mouse IL-5 Standard (lyophilized) 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml
FunctionFactor that induces terminal differentiation of late-developing B-cells to immunoglobulin secreting cells.
Sequence similaritiesBelongs to the IL-5 family.
- Information by UniProt
- B cell differentiation factor I
- B-cell differentiation factor I
- Colony stimulating factor eosinophil
SimpleStep ELISA kits
ab100711 has been referenced in 1 publication.
- Kong R & Yin J Efficacy of subcutaneous immunotherapy in a mouse model of Humulus pollen-induced allergic asthma. Am J Transl Res 11:280-289 (2019). PubMed: 30787986