Overview

  • Product name

    Mouse IL-6 ELISA Kit
    See all IL-6 kits
  • Detection method

    Colorimetric
  • Sample type

    Cell culture supernatant, Serum, Plasma
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    < 2 pg/ml
  • Range

    0.82 pg/ml - 600 pg/ml
  • Recovery

    94 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 96.18 86% - 103%
    Serum 93.39 83% - 103%
    Plasma 94.53 84% - 104%

  • Assay duration

    Multiple steps standard assay
  • Species reactivity

    Reacts with: Mouse
    Predicted to work with: Human
  • Product overview

    Mouse IL-6 ELISA kit is designed for the quantitative measurement of Mouse IL-6 in serum, plasma and cell culture supernatants.


    This assay employs an antibody specific for Mouse IL-6 coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-6 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Mouse IL-6 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-6 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Microplate

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components 1 x 96 tests
    200X HRP-Streptavidin Concentrate 1 x 200µl
    20X Wash Buffer Concentrate 1 x 25ml
    5X Assay Diluent 1 x 15ml
    Biotinylated anti-mouse IL-6 (Lyophilized) 2 vials
    IL-6 Microplate (12 x 8 wells) 1 unit
    Recombinant mouse IL-6 Standard (lyophilized) 2 vials
    Stop Solution 1 x 8ml
    TMB One-Step Substrate Reagent 1 x 12ml
  • Research areas

  • Function

    Cytokine with a wide variety of biological functions. It is a potent inducer of the acute phase response. Plays an essential role in the final differentiation of B-cells into Ig-secreting cells Involved in lymphocyte and monocyte differentiation. It induces myeloma and plasmacytoma growth and induces nerve cells differentiation Acts on B-cells, T-cells, hepatocytes, hematopoeitic progenitor cells and cells of the CNS. Also acts as a myokine. It is discharged into the bloodstream after muscle contraction and acts to increase the breakdown of fats and to improve insulin resistance.
  • Involvement in disease

    Genetic variations in IL6 are associated with susceptibility to rheumatoid arthritis systemic juvenile (RASJ) [MIM:604302]. An inflammatory articular disorder with systemic-onset beginning before the age of 16. It represents a subgroup of juvenile arthritis associated with severe extraarticular features and occasionally fatal complications. During active phases of the disorder, patients display a typical daily spiking fever, an evanescent macular rash, lymphadenopathy, hepatosplenomegaly, serositis, myalgia and arthritis.
    Note=A IL6 promoter polymorphism is associated with a lifetime risk of development of Kaposi sarcoma in HIV-infected men.
  • Sequence similarities

    Belongs to the IL-6 superfamily.
  • Post-translational
    modifications

    N- and O-glycosylated.
  • Cellular localization

    Secreted.
  • Information by UniProt
  • Alternative names

    • Interleukin BSF 2
    • B cell differentiation factor
    • B cell stimulatory factor 2
    • B-cell stimulatory factor 2
    • BSF 2
    • BSF-2
    • BSF2
    • CDF
    • CTL differentiation factor
    • Cytotoxic T cell differentiation factor
    • Hepatocyte stimulating factor
    • Hepatocyte stimulatory factor
    • HGF
    • HSF
    • Hybridoma growth factor
    • Hybridoma growth factor Interferon beta-2
    • Hybridoma plasmacytoma growth factor
    • IFN-beta-2
    • IFNB2
    • IL 6
    • IL-6
    • IL6
    • IL6_HUMAN
    • Interferon beta 2
    • Interferon beta-2
    • Interleukin 6
    • Interleukin 6 (interferon beta 2)
    • Interleukin BSF 2
    • Interleukin-6
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab100712 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • Standard curve of msIL-6 in different diluents with background signal subtracted (duplicates; +/- SD).
  • IL-6 measured in mouse tissue lysates (diluted in the range of 1:20 to 1:200; expressed as per mg of extracted protein), with background signal subtracted (duplicates; +/- SD).

  • msIL-6 measured in biological fluids, background signal subtracted (duplicates +/- SD).
  • IL-6 detected in supernatants from RAW 246.7 control cells (C) or cells stimulated for 24 hours with 50 ng x mL-1 of PMA (ab120297) (P), or 24 hours with PMA and 1 ug x mL-1 of LPS (Sigma) (P+L) for the last 6 hours. Results shown after background signal was subtracted (duplicates +/- SD).
  • Representative standard curve using ab100712

  • Representative standard curve using ab100712

Protocols

References

This product has been referenced in:

  • Zahednasab H  et al. The protective effect of rifampicin on behavioral deficits, biochemical, and neuropathological changes in a cuprizone model of demyelination. Cytokine 113:417-426 (2019). Read more (PubMed: 30539784) »
  • Yang X  et al. Precision toxicology shows that troxerutin alleviates ochratoxin A-induced renal lipotoxicity. FASEB J 33:2212-2227 (2019). Read more (PubMed: 30247986) »
See all 36 Publications for this product

Customer reviews and Q&As

1-3 of 3 Abreviews or Q&A

Answer

Gracias por tu respuesta.

No hay ningún problema en reemplazarte el kit al encontrarse éste en garantía. El nuevo pedido gratuito tiene referencia XXXX.

Te llegará un mail de confirmación del nuevo pedido, si necesitas información sobre el estado del mismo no dudes en contactarnos haciendo referencia al número indicado.

Al llevar a cabo el nuevo protocolo no olvides diluir la HRP Streptavidina en 1/200 en lugar de 1/10,000. El protocolo ya se ha actualizado para corregir el error.

Te pido disculpas por los inconvenientes, y espero que este nuevo kit funcione perfectamente.

No dudes en contactarnos de nuevo para cualquier otra consulta.

Read More

Answer

Te contacto de nuevo porque parece que hemos encontrado la fuente del error.

En el protocolo hay una incongruencia a la hora de especificar la dilución del vial de HRP Streptavidina. Primero indica que debe diluirse 10,000 veces en Assay Diluent B, para después en el ejemplo dado indicar que la dilución final corresponde a 1/200 (página 8).

Te sugiero que si puedes repitas el ensayo usando una dilución 1/200 y compruebes si los resultados obtenidos son mayores esta vez. Al estar el reactivo mas diluido los valores de absorbancia son comparables a los valores del background.

Si no tuvierais suficiente cantidad de reactivo para repetir el test, indícamelo para poder compensarte por el error.

Gracias por tu colaboración, y disculpa las molestias.

Read More

Answer

Te pido disculpas por la espera.

Para tratar de entender los resultados obtenidos estoy comentando con mis compañeros el protocolo seguido, y así asegurar que no volvéis a tener ningún problema con este kit.

Siendo que todas las absorbancias están al nivel del background, se descarta que haya habido degradación del estándar, ya que en ese caso, solo se hubieran obtenido valores bajos para el estándar.

¿Puedes por favor confirmarme los siguientes puntos?

-¿Cómo se diluyó el vial de HRP-Streptavidina?

-¿Qué anticoagulante se usó para preparar el plasma?

-¿Durante cuánto tiempo, y en qué condiciones se almacenaron las muestras?

-¿Se leyó la placa a 450nm inmediatamente después de añadir la Stop Solution?

-¿Se prepararon las diluciones seriadas del estándar tal y como figura en el protocolo? Sino, por favor indícame como se llevaron a cabo.

-¿Cómo se procedió al lavado de la placa? (pipeta simple, multi canal, limpiador automatizado, etc…)

Como ya comente en mi mail anterior, si resultara que alguno de los componentes del kit estuviera defectuoso, se os reemplazara o reembolsara el importe del mismo.

Agradezco mucho tu colaboración, y espero tu respuesta para poder resolver este problema con la mayor brevedad posible.

Read More

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