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    mouse-il-6-elisa-kit-ab222503.pdf

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Immunology Innate Immunity Cytokines Interleukins
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RecombinantSimpleStep

Mouse IL-6 ELISA Kit (ab222503)

  • Datasheet
  • SDS
  • Protocol Booklet
Reviews (2) Submit a question References (6)

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Other - Mouse IL-6 ELISA Kit (ab222503)
  • Example of mouse IL-6 standard curve.
  • Example of mouse IL-6 standard curve.
  • Interpolated concentrations of spiked IL-6 in mouse serum and plasma samples.
  • Interpolated concentrations of native IL-6 in mouse serum and plasma samples.
  • Interpolated concentrations of native IL-6 in mouse cell culture supernatant samples.
  • Linearity of dilution.
  • Linearity of dilution.
  • Sandwich ELISA - Mouse IL-6 ELISA Kit (ab222503)

Key features and details

  • One-wash 90 minute protocol
  • Sensitivity: 11.3 pg/ml
  • Range: 15.6 pg/ml - 1000 pg/ml
  • Sample type: Cell culture supernatant, Cit plasma, EDTA Plasma, Hep Plasma, Serum
  • Detection method: Colorimetric
  • Assay type: Sandwich (quantitative)
  • Reacts with: Mouse, Rat

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Overview

  • Product name

    Mouse IL-6 ELISA Kit
    See all IL-6 kits
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Spleen CCS 5 3.9%
    Inter-assay
    Sample n Mean SD CV%
    Spleen CCS 3 3.4%
  • Sample type

    Cell culture supernatant, Serum, Hep Plasma, EDTA Plasma, Cit plasma
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    11.3 pg/ml
  • Range

    15.6 pg/ml - 1000 pg/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Serum 107 103% - 114%
    Cell culture media 100 89% - 112%
    Hep Plasma 98 95% - 103%
    EDTA Plasma 112 109% - 116%
    Cit plasma 99 96% - 102%
  • Assay time

    1h 30m
  • Assay duration

    One step assay
  • Species reactivity

    Reacts with: Mouse, Rat
  • Product overview

    Mouse IL-6 ELISA Kit (ab222503) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of IL-6 protein in cell culture supernatant, cit plasma, edta plasma, hep plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse IL-6 with 11.3 pg/ml sensitivity.


    SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:


            - Single-wash protocol reduces assay time to 90 minutes or less
            - High sensitivity, specificity and reproducibility from superior antibodies
            - Fully validated in biological samples
            - 96-wells plate breakable into 12 x 8 wells strips


    A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.


    ASSAY SPECIFICITY


    This kit recognizes both native and recombinant mouse IL-6 protein in serum, plasma, and cell culture supernatant samples only. Cell and tissue extract samples have not been tested with this kit.


    SPECIES REACTIVITY


    This kit recognizes mouse and rat IL-6 protein.


    Rat IL-6 protein was measured at 250pg/mL and 100% cross reactivity was observed. Human IL-6 protein was measured at 1000pg/mL no cross reactivity was observed.

  • Notes

    Interleukin 6 (IL-6) is a cytokine with a wide variety of biological functions.  It is a potent inducer of the acute phase response and plays an essential role in the final differentiation of B-cells into Ig-secreting cells.  IL-6 is involved in lymphocyte and monocyte differentiation and IL-6 induces myeloma and plasmacytoma growth as well as nerve cells differentiation.  B-cells, T-cells, hepatocytes, hematopoeitic progenitor cells and cells of the CNS are all responsive to IL-6. IL-6 is discharged into the bloodstream after muscle contraction and acts to increase the breakdown of fats and to improve insulin resistance

  • Platform

    Pre-coated microplate (12 x 8 well strips)

Properties

  • Storage instructions

    Store at +4°C. Please refer to protocols.
  • Components 1 x 96 tests
    10X Mouse IL-6 Capture Antibody 1 x 600µl
    10X Mouse IL-6 Detector Antibody 1 x 600µl
    10X Wash Buffer PT (ab206977) 1 x 20ml
    Antibody Diluent 4BR 1 x 6ml
    Mouse IL-6 Lyophilized Recombinant Protein 2 vials
    Plate Seals 1 unit
    Sample Diluent NS (ab193972) 1 x 50ml
    SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit
    Stop Solution 1 x 12ml
    TMB Development Solution 1 x 12ml
  • Research areas

    • Immunology
    • Innate Immunity
    • Cytokines
    • Interleukins
    • Neuroscience
    • Neurology process
    • Metabolism
    • Cancer
    • Invasion/microenvironment
    • Angiogenesis
    • Angiogenic growth factors
    • Cancer
    • Tumor immunology
    • Cytokines
    • Interleukins
    • Metabolism
    • Types of disease
    • Obesity
    • Metabolism
    • Types of disease
    • Metabolic disorders
  • Function

    Cytokine with a wide variety of biological functions. It is a potent inducer of the acute phase response. Plays an essential role in the final differentiation of B-cells into Ig-secreting cells Involved in lymphocyte and monocyte differentiation. It induces myeloma and plasmacytoma growth and induces nerve cells differentiation Acts on B-cells, T-cells, hepatocytes, hematopoeitic progenitor cells and cells of the CNS. Also acts as a myokine. It is discharged into the bloodstream after muscle contraction and acts to increase the breakdown of fats and to improve insulin resistance.
  • Involvement in disease

    Genetic variations in IL6 are associated with susceptibility to rheumatoid arthritis systemic juvenile (RASJ) [MIM:604302]. An inflammatory articular disorder with systemic-onset beginning before the age of 16. It represents a subgroup of juvenile arthritis associated with severe extraarticular features and occasionally fatal complications. During active phases of the disorder, patients display a typical daily spiking fever, an evanescent macular rash, lymphadenopathy, hepatosplenomegaly, serositis, myalgia and arthritis.
    Note=A IL6 promoter polymorphism is associated with a lifetime risk of development of Kaposi sarcoma in HIV-infected men.
  • Sequence similarities

    Belongs to the IL-6 superfamily.
  • Post-translational
    modifications

    N- and O-glycosylated.
  • Cellular localization

    Secreted.
  • Target information above from: UniProt accession P05231 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Alternative names

    • Interleukin BSF 2
    • B cell differentiation factor
    • B cell stimulatory factor 2
    • B-cell stimulatory factor 2
    • BSF 2
    • BSF-2
    • BSF2
    • CDF
    • CTL differentiation factor
    • Cytotoxic T cell differentiation factor
    • Hepatocyte stimulating factor
    • Hepatocyte stimulatory factor
    • HGF
    • HSF
    • Hybridoma growth factor
    • Hybridoma growth factor Interferon beta-2
    • Hybridoma plasmacytoma growth factor
    • IFN-beta-2
    • IFNB2
    • IL 6
    • IL-6
    • IL6
    • IL6_HUMAN
    • Interferon beta 2
    • Interferon beta-2
    • Interleukin 6
    • Interleukin 6 (interferon beta 2)
    • Interleukin BSF 2
    • Interleukin-6
    see all
  • Database links

    • Entrez Gene: 16193 Mouse
    • Entrez Gene: 24498 Rat
    • SwissProt: P08505 Mouse
    • SwissProt: P20607 Rat
    • Unigene: 1019 Mouse
    • Unigene: 9873 Rat

    Associated products

      Images

      • Other - Mouse IL-6 ELISA Kit (ab222503)
        Other - Mouse IL-6 ELISA Kit (ab222503)

        SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

         

      • Example of mouse IL-6 standard curve.
        Example of mouse IL-6 standard curve.

        The IL-6 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

      • Example of mouse IL-6 standard curve.
        Example of mouse IL-6 standard curve.

        Example of mouse IL-6 standard curve. The IL-6 standard curve was prepared as described. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

      • Interpolated concentrations of spiked IL-6 in mouse serum and plasma samples.
        Interpolated concentrations of spiked IL-6 in mouse serum and plasma samples.

        The concentrations of IL-6 were measured in duplicates, interpolated from the IL-6 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 50%, plasma (heparin) 50%, plasma (EDTA) 50% and plasma (citrate) 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).

      • Interpolated concentrations of native IL-6 in mouse serum and plasma samples.
        Interpolated concentrations of native IL-6 in mouse serum and plasma samples.

        The concentrations of IL-6 were measured in duplicates, interpolated from the IL-6 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 50%, plasma (heparin) 50%, plasma (EDTA) 50% and plasma (citrate) 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IL-6 concentration was determined to be 39 pg/mL in serum, 42 pg/mL in plasma (heparin), 43 pg/mL in plasma (EDTA), and 32 pg/mL in plasma (citrate).

      • Interpolated concentrations of native IL-6 in mouse cell culture supernatant samples.
        Interpolated concentrations of native IL-6 in mouse cell culture supernatant samples.

        The concentrations of IL-6 were measured in duplicates, interpolated from the IL-6 standard curves and corrected for sample dilution. Undiluted samples are as follows: stimulated RAW264.7 cell culture supernatant 1:50 and stimulated spleen cell culture supernatant 1:16. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IL-6 concentration was determined to be 26,793 pg/mL in stimulated RAW264.7 cell culture supernatant, and 15,127 pg/mL in stimulated spleen cell culture supernatant. 

      • Linearity of dilution.
        Linearity of dilution.

        Linearity of dilution is determined based on interpolated values from the standard curve. Linearity of dilution defines a sample concentration interval in which interpolated target concentrations are directly proportional to sample dilution.

        Recombinant IL-6 was spiked into the following biological samples and diluted in a 2-fold dilution series in Sample Diluent NS.

      • Linearity of dilution.
        Linearity of dilution.

        Native IL-6 was measured in the following biological samples in a 2-fold dilution series. Sample dilutions are made in Sample Diluent NS.

      • Sandwich ELISA - Mouse IL-6 ELISA Kit (ab222503)
        Sandwich ELISA - Mouse IL-6 ELISA Kit (ab222503)
        To learn more about the advantages of recombinant antibodies see here.

      Protocols

      • Protocol Booklet

      Click here to view the general protocols

      Datasheets and documents

      • Datasheet
      • SDS
    • References (6)

      Publishing research using ab222503? Please let us know so that we can cite the reference in this datasheet.

      ab222503 has been referenced in 6 publications.

      • Wang YQ  et al. Gubenzhike Recipe Ameliorates Respiratory Mucosal Immunity in Mice with Chronic Obstructive Pulmonary Disease through Upregulation of the ?dT Lymphocytes and KGF Levels. Evid Based Complement Alternat Med 2020:3056797 (2020). PubMed: 32280354
      • Kim SM  et al. Role of Proinflammatory Cytokines in Feedback Modulation of Circadian Clock Gene Rhythms by Saturated Fatty Acids. Sci Rep 9:8909 (2019). PubMed: 31222133
      • Monga S  et al. Inhibitory Effects of the Two Novel TSPO Ligands 2-Cl-MGV-1 and MGV-1 on LPS-induced Microglial Activation. Cells 8:N/A (2019). PubMed: 31121852
      • Ren LR  et al. Staphylococcus Aureus Induces Osteoclastogenesis via the NF-?B Signaling Pathway. Med Sci Monit 23:4579-4590 (2017). PubMed: 28942456
      • Luo D  et al. Natural product celastrol suppressed macrophage M1 polarization against inflammation in diet-induced obese mice via regulating Nrf2/HO-1, MAP kinase and NF-?B pathways. Aging (Albany NY) 9:2069-2082 (2017). PubMed: 29040966
      • Pogue AI  et al. Systemic Inflammation in C57BL/6J Mice Receiving Dietary Aluminum Sulfate; Up-Regulation of the Pro-Inflammatory Cytokines IL-6 and TNFa, C-Reactive Protein (CRP) and miRNA-146a in Blood Serum. J Alzheimers Dis Parkinsonism 7:N/A (2017). PubMed: 29354323

      Customer reviews and Q&As

      Show All Reviews Q&A
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      1-2 of 2 Abreviews or Q&A

      IL-6 ELISA with mouse tissue lysates

      Good Excellent 5/5 (Ease of Use)
      Abreviews
      Abreviews
      abreview image
      Works good. Though, I didn't get very good standard curve but this kit works for mouse tissue samples (spleen and muscle tissue) and cell culture supernatant. Highly recommended.

      Miss. Sheelu Monga

      Verified customer

      Submitted Feb 21 2020

      IL-6 ELISA kit simple step

      Excellent Excellent 5/5 (Ease of Use)
      Abreviews
      Abreviews
      abreview image
      Very good kit and easy to use. I would highly recommend this kit.

      Miss. Sheelu Monga

      Verified customer

      Submitted Apr 27 2018

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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