• Product name
    Mouse IL-6 ELISA Kit (Interleukin-6)
    See all IL6 kits
  • Detection method
  • Sample type
    Cell culture extracts, Tissue Extracts
  • Assay type
    Sandwich (quantitative)
  • Sensitivity
    < 2 pg/ml
  • Range
    0.82 pg/ml - 600 pg/ml
  • Recovery

    93 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture extracts 94.53 84% - 104%
    Tissue Extracts 93.39 83% - 103%

  • Assay duration
    Multiple steps standard assay
  • Species reactivity
    Reacts with: Mouse
  • Product overview

    Abcam’s IL-6 Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse IL-6 in cell lysates and tissue lysates.

    This assay employs an antibody specific for mouse IL-6 coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-6 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse IL-6 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-6 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • Tested applications
    Suitable for: Sandwich ELISAmore details
  • Platform


  • Storage instructions
    Store at -20°C. Please refer to protocols.
  • Components 1 x 96 tests
    200X HRP-Streptavidin Concentrate 1 x 200µl
    20X Wash Buffer Concentrate 1 x 25ml
    2X Cell Lysate Buffer 1 x 5ml
    5X Assay Diluent 1 x 15ml
    5X Sample Diluent Buffer 1 x 10ml
    Biotinylated anti-mouse IL-6 (Lyophilized) 2 vials
    IL-6 Microplate (12 x 8 wells) 1 unit
    Recombinant mouse IL-6 Standard (lyophilized) 2 vials
    Stop Solution 1 x 8ml
    TMB One-Step Substrate Reagent 1 x 12ml
  • Research areas
  • Function
    Cytokine with a wide variety of biological functions. It is a potent inducer of the acute phase response. Plays an essential role in the final differentiation of B-cells into Ig-secreting cells Involved in lymphocyte and monocyte differentiation. It induces myeloma and plasmacytoma growth and induces nerve cells differentiation Acts on B-cells, T-cells, hepatocytes, hematopoietic progenitor cells and cells of the CNS. Also acts as a myokine. It is discharged into the bloodstream after muscle contraction and acts to increase the breakdown of fats and to improve insulin resistance.
  • Involvement in disease
    Genetic variations in IL6 are associated with susceptibility to rheumatoid arthritis systemic juvenile (RASJ) [MIM:604302]. An inflammatory articular disorder with systemic-onset beginning before the age of 16. It represents a subgroup of juvenile arthritis associated with severe extraarticular features and occasionally fatal complications. During active phases of the disorder, patients display a typical daily spiking fever, an evanescent macular rash, lymphadenopathy, hepatosplenomegaly, serositis, myalgia and arthritis.
    Note=A IL6 promoter polymorphism is associated with a lifetime risk of development of Kaposi sarcoma in HIV-infected men.
  • Sequence similarities
    Belongs to the IL-6 superfamily.
  • Post-translational
    N- and O-glycosylated.
  • Cellular localization
  • Information by UniProt
  • Alternative names
    • B cell differentiation factor
    • B cell stimulatory factor 2
    • B-cell stimulatory factor 2
    • BSF 2
    • BSF-2
    • BSF2
    • CDF
    • CTL differentiation factor
    • Cytotoxic T cell differentiation factor
    • Hepatocyte stimulating fact
    • HGF
    • HPGF
    • HSF
    • Hybridoma growth factor
    • Hybridoma plasmacytoma growth factor
    • IFN-beta-2
    • IFNB2
    • IL 6
    • IL-6
    • IL6
    • IL6 protein
    • IL6_HUMAN
    • Interferon beta 2
    • Interferon beta-2
    • Interleukin 6
    • Interleukin 6 (interferon beta 2)
    • Interleukin BSF 2
    • Interleukin-6
    • Interleukin6
    see all
  • Database links


Our Abpromise guarantee covers the use of ab100713 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.


  • IL-6 measured in mouse tissue lysates prepared with RIPA buffer and sonicated in an ice-cold water bath for 12 intervals of a total of 3.5 minutes. 0.02-1 mg protein x mL-1 were tested, results shown as IL-6 per mg of extracted protein (duplicates +/- SD).

  • IL-6 measured in RIPA lysates from control cells, or cells stimulated for 24 hours with 50 ng x mL-1 of PMA (ab120297), with the addition of 1 ug x mL-1 of LPS (Sigma) (P/L) for the last 6 hours. Samples were diluted and tested in the range of 1e4-5e5 cells x mL-1, results expressed per million cells; duplicates +/- SD).

  • Dilution curves of mouse IL-6 (ab100713) and NIBSC standard (93/730). One pg of standard mouse IL-6 corresponds to 0.37 (+/- 0.07) AU. Background signal subtracted (duplicates; +/- SD).

  • Standard curve of mouse IL-6 with background signal subtracted (duplicates; +/- SD).

  • Representative standard curve using ab100713



This product has been referenced in:
  • Liu B  et al. Root Extract of Polygonum cuspidatum Siebold & Zucc. Ameliorates DSS-Induced Ulcerative Colitis by Affecting NF-kappaB Signaling Pathway in a Mouse Model via Synergistic Effects of Polydatin, Resveratrol, and Emodin. Front Pharmacol 9:347 (2018). Read more (PubMed: 29695964) »
  • Piao X  et al. 1-Deoxynojirimycin (DNJ) Ameliorates Indomethacin-Induced Gastric Ulcer in Mice by Affecting NF-kappaB Signaling Pathway. Front Pharmacol 9:372 (2018). Read more (PubMed: 29725297) »

See all 11 Publications for this product

Customer reviews and Q&As

Thank you for getting in touch.

We have not tested ab100713 Mouse IL-6 ELISA Kit (Interleukin-6) specifically in liver samples in our own laboratories. However, I am happy to provide you with some general guidelines for tissue lysate preparati...

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IL6 in Rat liver tissue

Good Excellent 5/5 (Ease of Use)
The standard curve was as described as the typical curve. One concentration had to be eliminated to give a high R^2 value.
Samples were from rat (normal out-bred sprague dawley from Harlan) liver tissue, homogenized using a 1% np-40 buffer. Although a 1:10 dilution was used for the samples (which was sufficiently in range of the IL1b ELISA (ab100768) standards), the absorbances were out of range of the standards.
For the samples that did fall in the standard range, the levels of IL6 in control rats seem to be a little higher (~225-325pg/ml) than the expected range (~125-200pg/ml).

Abcam user community

Verified customer

Submitted Apr 02 2014

Thank you for your reply.

Just to clarify, the diluted/working standards (600, 200, 66.7, 22.2, 7.4, 2.5, 0.82 pg/ml) should not be stored. The reconstituted standard (50 ng/ml) can be stored at -80ºC for 1-2 weeks at most. Remember, an ...

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Thank you for your inquiry.

We know of the IL6 ELISA kit being tested in liver tissue lysate, but unfortunately we do not have any info on brain lysate.

For the SDF1 alpha ELISA kit, we haven't tested tissues with this kit but it wou...

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Thank you for contacting us.

The following ELISA kits for mouse ICAM, VCAM, IL-6 and TNF-alpha are suitable for mouse samples:

ab100688 (https://www.abcam.com/ICAM1-Mouse-ELISA-Kit-1-x-96-Well-Plate-ab100688.html)
ab100713 (ht...

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Thank you for contacting us this afternoon.

I have been to have a look at how the IL6 Mouse ELISA Kit (ab100713) looks and to take photos so that you could get a better idea of how it would work. The plate itself is a 96 well plate but can be...

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