Mouse Inflammation Antibody Array - Membrane (40 Targets) (ab133999)

Overview

  • Product name
    Mouse Inflammation Antibody Array - Membrane (40 Targets)
    See all Inflammation Antibody Array antibody arrays
  • Sample type
    Cell culture supernatant, Saliva, Milk, Urine, Serum, Plasma, Cell culture extracts, Other biological fluids, Whole Blood, Tissue Extracts, Cell Lysate, Cell culture media
  • Assay type
    Semi-quantitative
  • Species reactivity
    Reacts with: Mouse
  • Product overview

    ab133999 is for simultaneous detection of 40 Mouse inflammatory factors. Suitable for all sample types.


    Targets: BLC, CD30L, Eotaxin, Eotaxin-2, Fas Ligand, Fractalkine, GCSF, GM-CSF, IFN-gamma, IL-1alpha, IL-1beta, IL-2, IL-3, IL-4, IL-6, IL-9, IL-10, IL-12 p40/p70, IL-12 p70, IL-13, IL-17, I-TAC, KC/CXCL1, Leptin/OB, LIX, Lymphotactin, MCP-1, MCSF, MIG, MIP-1 alpha, MIP-1 gamma, RANTES, SDF-1, TCA-3, TECK, TIMP-1, TIMP-2, TNF-alpha, sTNF RI, sTNF-RII


    Cytokine arrays are an antibody-pair-based assay, analogous to ELISA, but using a membrane as a substrate rather than a plate. Capture antibodies are supplied arrayed/spotted on a membrane with each pair of spots representing a different analyte. Sample is added (0.2-1ml of 1 sample to each membrane), and then paired biotinylated detector antibodies and streptavidin HRP. The cytokine array is analyzed using the same methods as a chemiluminescent western blot. Comparison between samples can be by eye or using densitometry software for a semi-quantitative comparison.


    Learn more about membrane antibody arrays

  • Notes

    A table listing all of our mouse membrane antibody cytokine arrays and other arrays and the analytes they measure is available here.

  • Tested applications
    Suitable for: Multiplex Protein Detectionmore details

Properties

Applications

Our Abpromise guarantee covers the use of ab133999 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Multiplex Protein Detection Use at an assay dependent concentration.

Images

  • Typical results obtained with Abcam Membrane Antibody Arrays.
    Membranes were probed with conditioned media from two different cell lines. Membranes were exposed to film at RT for 1 min.

Protocols

References

This product has been referenced in:
  • Crespo Yanguas S  et al. TAT-Gap19 and Carbenoxolone Alleviate Liver Fibrosis in Mice. Int J Mol Sci 19:N/A (2018). Read more (PubMed: 29534516) »
  • Al-Dwairi A  et al. Glucagon-like peptide-1 exerts anti-inflammatory effects on mouse colon smooth muscle cells through the cyclic adenosine monophosphate/nuclear factor-?B pathway in vitro. J Inflamm Res 11:95-109 (2018). Read more (PubMed: 29593427) »
See all 5 Publications for this product

Customer reviews and Q&As

1-2 of 2 Q&A

Question
Answer


We do carry 2antibody arrays that are specific for rat:

ab133991: https://www.abcam.com/cytokine-rat-membrane-antibody-array-19-targets-ab133991.html

ab133992: https://www.abcam.com/cytokine-rat-membrane-antibody-array-34-targets-ab133992.html

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Answer

Gracias por tu respuesta.
Las membranas son de un solo uso. Cada membrana es válida para una sola muestra.
Existe la posibilidad de adquirir 4 o 8 membranas. Con 4 membranas puede usarse una para el control positivo, una para el control negativo, y dos para muestras.
Espero haberte ayudado. Si tienes más consultas, no dudes en volverme a contactar.

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