Key features and details
- Sensitivity: 5 µlU/ml
- Range: 6.25 µlU/ml - 400 µlU/ml
- Sample type: Cell culture supernatant, Cit plasma, EDTA Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
Product nameMouse Insulin ELISA Kit
See all Insulin kits
Intra-assay Sample n Mean SD CV% Overall < 10% Inter-assay Sample n Mean SD CV% Overall < 12%
Sample typeCell culture supernatant, Serum, EDTA Plasma, Cit plasma
Assay typeSandwich (quantitative)
Range6.25 µlU/ml - 400 µlU/ml
Sample specific recovery Sample type Average % Range Serum 111.2 90% - 132% Plasma 89.67 70% - 109% Cell culture media 108.5 80% - 139%
Assay durationMultiple steps standard assay
Mouse Insulin ELISA Kit (ab277390) is an in-vitro enzyme-linked immunosorbent assay for the quantitative measurement of Mouse Insulin in serum, plasma (Collect plasma using EDTA and Citrate as an anticoagulant. Heparin is not recommended as anticoagulants for use in this assay) and cell culture supernatants.
This assay employs an antibody specific for Mouse Insulin coated on a 96-well plate. Standards and samples are pipetted into the wells and Mouse Insulin present in a sample is bound to the wells by the immobilized antibody. The wells are washed, and biotinylated anti-Mouse Insulin antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Mouse Insulin bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
PlatformPre-coated microplate (12 x 8 well strips)
Storage instructionsStore at -20°C. Please refer to protocols.
Components 1 x 96 tests 20X Wash Buffer Concentrate 1 x 25ml 400X HRP-Streptavidin Concentrate 1 x 200µl 5X Assay Diluent B 1 x 15ml Assay Diluent C 1 x 30ml Biotinylated Anti-Mouse Insulin Antibody 2 vials Anti-Mouse Insulin coated Microplate 1 unit Mouse Insulin standard protein (Lyophilized) 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml
FunctionInsulin decreases blood glucose concentration. It increases cell permeability to monosaccharides, amino acids and fatty acids. It accelerates glycolysis, the pentose phosphate cycle, and glycogen synthesis in liver.
Involvement in diseaseDefects in INS are the cause of familial hyperproinsulinemia (FHPRI) [MIM:176730].
Defects in INS are a cause of diabetes mellitus insulin-dependent type 2 (IDDM2) [MIM:125852]. IDDM2 is a multifactorial disorder of glucose homeostasis that is characterized by susceptibility to ketoacidosis in the absence of insulin therapy. Clinical fetaures are polydipsia, polyphagia and polyuria which result from hyperglycemia-induced osmotic diuresis and secondary thirst. These derangements result in long-term complications that affect the eyes, kidneys, nerves, and blood vessels.
Defects in INS are a cause of diabetes mellitus permanent neonatal (PNDM) [MIM:606176]. PNDM is a rare form of diabetes distinct from childhood-onset autoimmune diabetes mellitus type 1. It is characterized by insulin-requiring hyperglycemia that is diagnosed within the first months of life. Permanent neonatal diabetes requires lifelong therapy.
Defects in INS are a cause of maturity-onset diabetes of the young type 10 (MODY10) [MIM:613370]. MODY10 is a form of diabetes that is characterized by an autosomal dominant mode of inheritance, onset in childhood or early adulthood (usually before 25 years of age), a primary defect in insulin secretion and frequent insulin-independence at the beginning of the disease.
Sequence similaritiesBelongs to the insulin family.
- Information by UniProt
ab277390 has not yet been referenced specifically in any publications.