Overview

  • Product name

    Mouse Interferon gamma ELISA Kit
    See all Interferon gamma kits
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    A 20 315.4pg/ml 14.5 4.6%
    B 19 588.6pg/ml 19.1 4.6%
    Inter-assay
    Sample n Mean SD CV%
    A 9 128.8pg/ml 7.4 5.7%
    B 10 546.9pg/ml 25.2 4.6%
  • Sample type

    Milk, Serum
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    < 15 pg/ml
  • Range

    31.25 pg/ml - 1000 pg/ml
  • Assay time

    3h 45m
  • Assay duration

    Multiple steps standard assay
  • Species reactivity

    Reacts with: Mouse
  • Product overview

    Mouse interferon gamma ELISA kit (IFN Gamma) is designed for the quantitative measurement of IFN gamma in mouse serum, buffered solutions and cell culture medium.


    A monoclonal antibody specific for IFN gamma has been coated onto the wells of the microtiter strips provided. Samples, including standards of known IFN gamma concentrations, control specimens or unknowns are pipetted into these wells. During the first incubation, the standards or samples and a biotinylated monoclonal antibody specific for IFN gamma are simultaneously incubated. After washing, the enzyme Streptavidin-HRP, that binds the biotinylated antibody is added, incubated and washed. A TMB substrate solution is added which acts on the bound enzyme to induce a colored reaction product. The intensity of this colored product is directly proportional to the concentration of IFN gamma present in the samples.


    This kit will recognize both endogenous and recombinant mouse IFN gamma.


    Get results in 90 minutes with Human IFN gamma ELISA Kit (ab174443) from our SimpleStep ELISA® range.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Microplate

Properties

  • Storage instructions

    Store at +4°C. Please refer to protocols.
  • Components Identifier 1 x 96 tests 2 x 96 tests
    10X Standard Diluent Buffer Black 1 x 25ml 1 x 25ml
    200X Wash Buffer White 1 x 10ml 2 x 10ml
    Biotinylated Antibody Diluent Red 1 x 7.5ml 1 x 13ml
    IFNγ Biotinylated detection antibody 1 x 400µl 2 x 400µl
    Chromogen TMB Substrate Solution 1 x 11ml 1 x 24ml
    HRP Diluent Red 1 x 23ml 1 x 23ml
    IFN gamma Microplate (12 x 8 well strips) 1 unit 2 units
    IFN gamma Standard (Lyophilized) Yellow 2 vials 4 vials
    Stop Reagent Black 1 x 11ml 2 x 11ml
    Streptavidin-HRP 2 x 5µl 4 x 5µl
  • Research areas

  • Function

    Produced by lymphocytes activated by specific antigens or mitogens. IFN-gamma, in addition to having antiviral activity, has important immunoregulatory functions. It is a potent activator of macrophages, it has antiproliferative effects on transformed cells and it can potentiate the antiviral and antitumor effects of the type I interferons.
  • Tissue specificity

    Released primarily from activated T lymphocytes.
  • Involvement in disease

    In Caucasians, genetic variation in IFNG is associated with the risk of aplastic anemia (AA) [MIM:609135]. AA is a rare disease in which the reduction of the circulating blood cells results from damage to the stem cell pool in bone marrow. In most patients, the stem cell lesion is caused by an autoimmune attack. T-lymphocytes, activated by an endogenous or exogenous, and most often unknown antigenic stimulus, secrete cytokines, including IFN-gamma, which would in turn be able to suppress hematopoiesis.
  • Sequence similarities

    Belongs to the type II (or gamma) interferon family.
  • Post-translational
    modifications

    Proteolytic processing produces C-terminal heterogeneity, with proteins ending alternatively at Gly-150, Met-157 or Gly-161.
  • Cellular localization

    Secreted.
  • Information by UniProt
  • Alternative names

    • IF 1
    • IFG
    • IFI
    • IFN gamma
    • IFN immune
    • IFN, immune
    • IFN-gamma
    • IFNG
    • IFNG_HUMAN
    • Immune interferon
    • Interferon gamma
    • Type II Interferon
    see all
  • Database links

Associated products

Applications

Our Abpromise guarantee covers the use of ab46081 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  •  Representative Standard Curve using ab46081

Protocols

References

This product has been referenced in:

  • Hajmohammadi M  et al. Synthesis and characterization of a novel chemically designed (Globo)3-DTPA-KLH antigen. Drug Des Devel Ther 9:217-39 (2015). Sandwich ELISA ; Mouse . Read more (PubMed: 25565775) »
See 1 Publication for this product

Customer reviews and Q&As

1-7 of 7 Abreviews or Q&A

Answer

Gracias por contactarnos.


He querido comprobar la posibilidad de presentar reactividad cruzada entre ratón y humano en los kits contra las citoquinas que mencionas.

Tenemos varios kits contra dichas citoquinas específicos para ratón, y específicos para humano. Después de estudiar la homología entre secuencias, me temo que no podemos predecir que exista reactividad cruzada entre especies. Experimentalmente tampoco tenemos datos para confirmarlo.

Siento no poder ser de mas ayuda. No dudes en contactarnos para cualquier otra consulta.

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Answer



Unfortunately, this kit is a ELISpot assay dedicated to detect IFNg secreting cells and not appropriate for serum.

This product will work for your customer's samples:

https://www.abcam.com/index.html?datasheet=46081 (or use the following: https://www.abcam.com/index.html?datasheet=46081).


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Question
Answer

Thank you for contacting us.

The HRP-streptavidin is available in catalogue. Please buy the product ab96467.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Use our products? Submit an Abreview. Earn rewards!
https://www.abcam.com/abreviews

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Question
Answer

Thank you for contacting us. We still had your email address wrong. Now it is fine, you should receiving my emails.

To let you know I have requested information from the lab regarding the HRP-strepatavidin conjugateand now waiting a reply from them. I will get back to you soon.

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Question
Answer

I have requested information from the lab and now waiting a reply.

I will get back to you soon.

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Answer

Thank you for contacting us.

I have had the chance to speak with the lab regarding your inquiry.Regrettably, the standards includedwithin the Interferon gamma Mouse ELISA kit are not correlated with Biological activity Unit; these tests have not been performed.


I apologize for any inconvenience this may cause. Please do not hesitate to contact us if you need any more advice or information.

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Abreview for Interferon gamma Mouse ELISA Kit

Excellent Excellent 5/5 (Ease of Use)
Abreviews

Sample: Mouse Serum (Plasma)
Specification: Plasma
Amount of Sample: 10 µg
Type of Kit Used: ELISA
Positive control: C57Bl/6 male mice 8 weeks of age were treated with LPS IP 1mg/kg (0111:B4). After 4 hours blood was collected in EDTA coated tubes. 1:10 dilutions of plasma were made with standard dilutend and used for ELISA. All samples (1:10 diluted) were in range of the standard curve.
Did you use the Abcam Kit protocol?: Yes

Mr. Thomas Milsom

Verified customer

Submitted Feb 24 2009

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