Mouse Interferon gamma ELISA Kit (ab46081)
Key features and details
- Sensitivity: 15 pg/ml
- Range: 31.25 pg/ml - 1000 pg/ml
- Sample type: Milk, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
Overview
-
Product name
Mouse Interferon gamma ELISA Kit
See all Interferon gamma kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% A 20 315.4pg/ml 14.5 4.6% B 19 588.6pg/ml 19.1 4.6% Inter-assay Sample n Mean SD CV% A 9 128.8pg/ml 7.4 5.7% B 10 546.9pg/ml 25.2 4.6% -
Sample type
Milk, Serum -
Assay type
Sandwich (quantitative) -
Sensitivity
< 15 pg/ml -
Range
31.25 pg/ml - 1000 pg/ml -
Assay time
3h 45m -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Mouse -
Product overview
Mouse interferon gamma ELISA kit (IFN Gamma) is designed for the quantitative measurement of IFN gamma in mouse serum, buffered solutions and cell culture medium.
A monoclonal antibody specific for IFN gamma has been coated onto the wells of the microtiter strips provided. Samples, including standards of known IFN gamma concentrations, control specimens or unknowns are pipetted into these wells. During the first incubation, the standards or samples and a biotinylated monoclonal antibody specific for IFN gamma are simultaneously incubated. After washing, the enzyme Streptavidin-HRP, that binds the biotinylated antibody is added, incubated and washed. A TMB substrate solution is added which acts on the bound enzyme to induce a colored reaction product. The intensity of this colored product is directly proportional to the concentration of IFN gamma present in the samples.
This kit will recognize both endogenous and recombinant mouse IFN gamma.
Get results in 90 minutes with Human IFN gamma ELISA Kit (ab174443) from our SimpleStep ELISA® range.
-
Platform
Microplate
Properties
-
Storage instructions
Store at +4°C. Please refer to protocols. -
Components Identifier 1 x 96 tests 2 x 96 tests 10X Standard Diluent Buffer Black 1 x 25ml 1 x 25ml 200X Wash Buffer White 1 x 10ml 2 x 10ml Biotinylated Antibody Diluent Red 1 x 7.5ml 1 x 13ml Chromogen TMB Substrate Solution 1 x 11ml 1 x 24ml HRP Diluent Red 1 x 23ml 1 x 23ml IFN gamma Microplate (12 x 8 well strips) 1 unit 2 units IFN gamma Standard (Lyophilized) Yellow 2 vials 4 vials IFNγ Biotinylated detection antibody 1 x 400µl 2 x 400µl Stop Reagent Black 1 x 11ml 2 x 11ml Streptavidin-HRP 2 x 5µl 4 x 5µl -
Research areas
-
Function
Produced by lymphocytes activated by specific antigens or mitogens. IFN-gamma, in addition to having antiviral activity, has important immunoregulatory functions. It is a potent activator of macrophages, it has antiproliferative effects on transformed cells and it can potentiate the antiviral and antitumor effects of the type I interferons. -
Tissue specificity
Released primarily from activated T lymphocytes. -
Involvement in disease
In Caucasians, genetic variation in IFNG is associated with the risk of aplastic anemia (AA) [MIM:609135]. AA is a rare disease in which the reduction of the circulating blood cells results from damage to the stem cell pool in bone marrow. In most patients, the stem cell lesion is caused by an autoimmune attack. T-lymphocytes, activated by an endogenous or exogenous, and most often unknown antigenic stimulus, secrete cytokines, including IFN-gamma, which would in turn be able to suppress hematopoiesis. -
Sequence similarities
Belongs to the type II (or gamma) interferon family. -
Post-translational
modificationsProteolytic processing produces C-terminal heterogeneity, with proteins ending alternatively at Gly-150, Met-157 or Gly-161. -
Cellular localization
Secreted. - Information by UniProt
-
Alternative names
- IF 1
- IFG
- IFI
see all -
Database links
- Entrez Gene: 15978 Mouse
- SwissProt: P01580 Mouse
- Unigene: 240327 Mouse
Associated products
-
SimpleStep ELISA kits
Images
-
Typical Standard Curve
Representative Standard Curve using ab46081
Datasheets and documents
-
SDS download
-
Datasheet download
References (4)
ab46081 has been referenced in 4 publications.
- Khodayari H et al. Gamma-radiated immunosuppressed tumor xenograft mice can be a new ideal model in cancer research. Sci Rep 11:256 (2021). PubMed: 33420261
- Menga A et al. Glufosinate constrains synchronous and metachronous metastasis by promoting anti-tumor macrophages. EMBO Mol Med N/A:e11210 (2020). PubMed: 32885605
- Sousa-Victor P et al. MANF regulates metabolic and immune homeostasis in ageing and protects against liver damage. Nat Metab 1:276-290 (2019). PubMed: 31489403
- Hajmohammadi M et al. Synthesis and characterization of a novel chemically designed (Globo)3-DTPA-KLH antigen. Drug Des Devel Ther 9:217-39 (2015). Sandwich ELISA ; Mouse . PubMed: 25565775