Overview

  • Product name
    Mouse Interferon gamma ELISA Set (without plates)
    See all Interferon gamma kits
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay type
    Sandwich (quantitative)
  • Assay duration
    Multiple steps standard assay
  • Species reactivity
    Reacts with: Mouse
  • Product overview

    Abcam’s IFNγ Murine in vitro ELISA Set is designed for the quantitative measurement of Murine IFNγ in cell culture supernatants, buffered solutions, serum, plasma and other body fluids.

     

    The murine IFN-gamma ELISA Set includes both the capture and the detection antibodies as well as Horse-Radish peroxidase conjugated Streptavidin (HRP-Strep) and TMB which are the reagents used in the colour development step.

     

    This ELISA Set is intended to measure murine IFN-gamma in cell -culture supernatants, as well as in serum samples. The enclosed procedures provide general guidelines for ELISA preparation. This procedure may need some optimization depending on the plastic, matrix, standard range.

     

     Recognizes both natural and recombinant Murine Interferon gamma Range: 31.25 pg/ml - 1000 pg/ml Sensitivity < 15 pg/ml.

  • Tested applications
    Suitable for: Sandwich ELISAmore details
  • Platform
    Reagents

Properties

  • Storage instructions
    Store at +4°C. Please refer to protocols.
  • Components Identifier 5 x 96 tests 10 x 96 tests 15 x 96 tests
    Detection biotinylated anti-Murine IFN gamma antibody 1 x 0.55ml 2 x 0.55ml 3 x 0.55ml
    Murine IFN gamma Capture Antibody 1 x 0.5ml 2 x 0.5ml 3 x 0.5ml
    IFN gamma Standard (Lyophilized) Yellow 5 vials 10 vials 15 vials
    Chromogen TMB Substrate Solution 2 vials 4 vials 6 vials
    Streptavidin-HRP 1 vial 2 vials 3 vials
  • Research areas
  • Function
    Produced by lymphocytes activated by specific antigens or mitogens. IFN-gamma, in addition to having antiviral activity, has important immunoregulatory functions. It is a potent activator of macrophages, it has antiproliferative effects on transformed cells and it can potentiate the antiviral and antitumor effects of the type I interferons.
  • Tissue specificity
    Released primarily from activated T lymphocytes.
  • Involvement in disease
    In Caucasians, genetic variation in IFNG is associated with the risk of aplastic anemia (AA) [MIM:609135]. AA is a rare disease in which the reduction of the circulating blood cells results from damage to the stem cell pool in bone marrow. In most patients, the stem cell lesion is caused by an autoimmune attack. T-lymphocytes, activated by an endogenous or exogenous, and most often unknown antigenic stimulus, secrete cytokines, including IFN-gamma, which would in turn be able to suppress hematopoiesis.
  • Sequence similarities
    Belongs to the type II (or gamma) interferon family.
  • Post-translational
    modifications
    Proteolytic processing produces C-terminal heterogeneity, with proteins ending alternatively at Gly-150, Met-157 or Gly-161.
  • Cellular localization
    Secreted.
  • Information by UniProt
  • Alternative names
    • IFG
    • IFI
    • IFN gamma
    • IFN, immune
    • IFN-gamma
    • IFNG
    • IFNG_HUMAN
    • Immune interferon
    • Interferon gamma
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab47618 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • Murine IFN-gamma Standard curve ranging from 31.25 to 1000 pg/mL

References

ab47618 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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