Product nameMouse Interferon gamma ELISA Set (without plates)
See all Interferon gamma kits
Sample typeCell culture supernatant, Serum, Plasma
Assay typeSandwich (quantitative)
Assay durationMultiple steps standard assay
Species reactivityReacts with: Mouse
Abcam’s IFNγ Murine in vitro ELISA Set is designed for the quantitative measurement of Murine IFNγ in cell culture supernatants, buffered solutions, serum, plasma and other body fluids.
The murine IFN-gamma ELISA Set includes both the capture and the detection antibodies as well as Horse-Radish peroxidase conjugated Streptavidin (HRP-Strep) and TMB which are the reagents used in the colour development step.
This ELISA Set is intended to measure murine IFN-gamma in cell -culture supernatants, as well as in serum samples. The enclosed procedures provide general guidelines for ELISA preparation. This procedure may need some optimization depending on the plastic, matrix, standard range.
Recognizes both natural and recombinant Murine Interferon gamma Range: 31.25 pg/ml - 1000 pg/ml Sensitivity < 15 pg/ml.
Tested applicationsSuitable for: Sandwich ELISAmore details
Storage instructionsStore at +4°C. Please refer to protocols.
Components Identifier 5 x 96 tests 10 x 96 tests 15 x 96 tests Detection biotinylated anti-Murine IFN gamma antibody 1 x 0.55ml 2 x 0.55ml 3 x 0.55ml Murine IFN gamma Capture Antibody 1 x 0.5ml 2 x 0.5ml 3 x 0.5ml IFN gamma Standard (Lyophilized) Yellow 5 vials 10 vials 15 vials Chromogen TMB Substrate Solution 2 vials 4 vials 6 vials Streptavidin-HRP 1 vial 2 vials 3 vials
FunctionProduced by lymphocytes activated by specific antigens or mitogens. IFN-gamma, in addition to having antiviral activity, has important immunoregulatory functions. It is a potent activator of macrophages, it has antiproliferative effects on transformed cells and it can potentiate the antiviral and antitumor effects of the type I interferons.
Tissue specificityReleased primarily from activated T lymphocytes.
Involvement in diseaseIn Caucasians, genetic variation in IFNG is associated with the risk of aplastic anemia (AA) [MIM:609135]. AA is a rare disease in which the reduction of the circulating blood cells results from damage to the stem cell pool in bone marrow. In most patients, the stem cell lesion is caused by an autoimmune attack. T-lymphocytes, activated by an endogenous or exogenous, and most often unknown antigenic stimulus, secrete cytokines, including IFN-gamma, which would in turn be able to suppress hematopoiesis.
Sequence similaritiesBelongs to the type II (or gamma) interferon family.
modificationsProteolytic processing produces C-terminal heterogeneity, with proteins ending alternatively at Gly-150, Met-157 or Gly-161.
- Information by UniProt
- IF 1
Our Abpromise guarantee covers the use of ab47618 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Sandwich ELISA||Use at an assay dependent concentration.|
ab47618 has not yet been referenced specifically in any publications.