Mouse Interleukin Antibody Array (30 Targets) - Quantitative (ab197474)

Overview

  • Product name

    Mouse Interleukin Antibody Array (30 Targets) - Quantitative
    See all Interleukin Antibody Array antibody arrays
  • Sample type

    Cell culture supernatant, Serum, Plasma, Other biological fluids, Cell Lysate, Tissue Lysate
  • Assay type

    Quantitative
  • Assay time

    6h 00m
  • Species reactivity

    Reacts with: Mouse
  • Product overview

    ab197474 can be used for quantitative measurement of 40 mouse interleukins. Suitable for cell culture supernatants, plasma, serum, cell and tissue lysates and other biological fluids. 

    Targets names: G-CSF, GM-CSF, IFNγ, IL-1α, IL-1β, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-9, IL-10, IL-12p70, IL-13, IL-15, IL-17, IL-21, IL-23, TNFα, IL-1ra, IL-2Rα, IL-6R, IL-11 , IL-12p40, IL-17B, IL-17E, IL-17F, IL-20, IL-28. 

    Learn more about quantitative antibody arrays

  • Notes

    Quantitative antibody arrays can be used to quantitate up to 40 cytokines with as little as 50 µL of sample. Arrays are available for 400 human and 200 mouse proteins. 

    Each glass slide is spotted with 16 identical antibody arrays and is provided with a 16 well gasket to allow separate samples to be applied to each array. Each antibody is spotted in quadruplicate in each array. For reproducible quantitation, eight of the arrays are used with a cocktail of protein standards to produce a standard curve. The same 8 standard curve arrays can be used across multiple slides, allowing measurement of 22 experimental samples with 2 slides, 36 with 3 slides etc. 

    For high through-put, 4 slides can be nested into a tray matching a standard microplate, allowing automated processing with a liquid handling workstation. 

    Array processing can be completed within one working day (see workflow diagram below) and arrays can be analyzed with a wide number of laser glass slide array / gene microarray scanners. If you don’t have a suitable scanner then we recommend our membrane antibody arrays, which can be analyzed with any WB chemiluminescent reader. 

    Learn more about quantitative antibody arrays 

    Learn more about membrane antibody arrays

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components 8 tests
    20X Wash Buffer I 3 x 30ml
    20X Wash Buffer II 1 x 30ml
    Cy3 equivalent dye-conjugated Streptavidin 2 x 5µl
    Mouse Interleukin Array (10 T) Biotinylated Antibody Cocktail 1 unit
    Mouse Interleukin Array (10 T) Glass Slide 1 slide
    Mouse Interleukin Array (10 T) Lyophilized Cytokine Standard Mix 1 vial
    Mouse Interleukin Array (20 T) Biotinylated Antibody Cocktail 1 unit
    Mouse Interleukin Array (20 T) Glass Slide 1 slide
    Mouse Interleukin Array (20 T) Lyophilized Cytokine Standard Mix 1 vial
    Sample Diluent 1 x 15ml
    Slide Washer/Dryer (30 mL Centrifuge Tube) 1 unit

Images

  • Mouse Interleukin Antibody Array - Quantitative (30 targets) (ab197474) Assay Summary

Protocols

References

ab197474 has not yet been referenced specifically in any publications.

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