Key features and details
- One-wash 90 minute protocol
- Sensitivity: 21.75 pg/ml
- Range: 64 pg/ml - 4100 pg/ml
- Sample type: Cell culture supernatant, Cit plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
Product nameMouse IP-10 ELISA Kit (CXCL10)
See all IP10 kits
Intra-assay Sample n Mean SD CV% Supernatant 8 8% Inter-assay Sample n Mean SD CV% Supernatant 3 10.6%
Sample typeCell culture supernatant, Serum, Cit plasma
Assay typeSandwich (quantitative)
Range64 pg/ml - 4100 pg/ml
Sample specific recovery Sample type Average % Range Cell culture supernatant 104 93% - 115% Serum 95 92% - 102% Cit plasma 89 84% - 99%
Assay time1h 30m
Assay durationOne step assay
Species reactivityReacts with: Mouse
Mouse IP-10 ELISA Kit (CXCL10) (ab260067) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of IP-10 (CXCL10) protein in cell culture supernatant, cit plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse IP-10 (CXCL10) with 21.75 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
ASSAY SPECIFICITY: This kit recognizes both native and recombinant mouse IP-10 protein in serum, citrate plasma, and cell culture supernatant samples only.
Cell and tissue extract samples have not been tested with this kit.
This kit is incompatible with heparin plasma and EDTA plasma samples.
CROSS REACTIVITY: Recombinant human IP-10 and rat IP-10 were each prepared at 50 ng/mL and 1.5 ng/mL and assayed for cross reactivity. No cross reactivity was observed.
SPECIES REACTIVITY: This kit recognizes mouse IP-10 protein.
No signal was observed in 50% serum samples from the following species: Human, Rat, Cow.
C-X-C motif chemokine 10 (CXCL10 or IP-10) is a small 10.8kD protein that is secreted by several cell types in response to interferon-gamma (IFNg). These cell types include monocytes, endothelial cells and fibroblasts. Upon secretion, CXCL10 is cleaved into an 8.7kD biologically active protein to function in chemotaxis for T-cells, NK cells, monocytes/macrophages and dendritic cells. In addition, CXCL10 has antitumor activity through the inhibition of bone marrow colony formation and angiogenesis. CXCL10 elicits its effects by binding to the cell surface chemokine receptor 3 (CXCR3).
PlatformPre-coated microplate (12 x 8 well strips)
Storage instructionsStore at +4°C. Please refer to protocols.
Components 1 x 96 tests 10X Mouse IP-10 (CXCL10) Capture Antibody 1 x 600µl 10X Mouse IP-10 (CXCL10) Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml Antibody Diluent 5BR 1 x 6ml Mouse IP-10 (CXCL10) Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 12ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml
FunctionChemotactic for monocytes and T-lymphocytes. Binds to CXCR3.
Sequence similaritiesBelongs to the intercrine alpha (chemokine CxC) family.
modificationsCXCL10(1-73) is produced by proteolytic cleavage after secretion from keratinocytes.
- Information by UniProt
- Interferon gamma induced factor MOB1, mouse, homolog of
- Interferon gamma induced protein 10
- 10 kDa interferon gamma induced protein
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
The IP-10 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
Example of mouse IP-10 standard curve in Sample Diluent NS. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
The concentrations of IP-10 were measured in duplicates, interpolated from the IP-10 standard curves and corrected for sample dilution. Undiluted samples are as follows: RAW264.7 supernatant 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IP-10 concentration was determined to be 442.08 pg/mL in RAW264.7 supernatant.
Linearity of dilution is determined based on interpolated values from the standard curve. Linearity of dilution defines a sample concentration interval in which interpolated target concentrations are directly proportional to sample dilution.
Native IP-10 was measured in the following biological samples in a 2-fold dilution series. Sample dilutions are made in Sample Diluent NS.
Recombinant IP-10 was spiked into the following biological samples and diluted in a 2-fold dilution series in Sample Diluent NS.
Neat pooled serum and plasma (citrate) samples from healthy donors was measured in duplicate. All values were below the detectable range of the assay.
ab260067 has not yet been referenced specifically in any publications.