Mouse IP-10 Matched Antibody Pair Kit (ab275364)
Overview
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Product name
Mouse IP-10 Matched Antibody Pair Kit
See all IP10 kits -
Detection method
Colorimetric -
Assay type
ELISA set -
Sensitivity
0.168 pg/ml -
Range
7.8 pg/ml - 500 pg/ml -
Species reactivity
Reacts with: Mouse -
Product overview
Mouse IP-10 Matched Antibody Pair Kits include a capture and a biotinylated detector antibody pair, along with a calibrated protein standard, suitable for sandwich ELISA. The Matched Antibody Pair Kit can be used to quantify native and recombinant mouse IP-10.
Optimization of the kit reagents to sample type, immunoassay format or instrumentation may be required. Guidelines for use of this kit in a standard 96-well microplate sandwich ELISA using HRP/TMB system of colorimetric detection is described in this assay procedure for the purposes of quantification.
Protocol information and tips on the use of the Matched Antibody Pair kits for sandwich ELISA can be found on our website. An accessory pack can be purchased which includes buffer reagents required to perform 10 x 96-well plate sandwich ELISAs (ab210905).
For additional information on the performance of the antibody pair used in this kit, please see our equivalent SimpleStep ELISA kit ab260067. Please note that while the antibody pair is the same provided in the corresponding SimpleStep ELISA Kit, due to differences in their formulation, this antibody pair cannot be used with the consumables provided with our SimpleStep ELISA Kits.
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Tested applications
Suitable for: IA, ELISAmore details -
Platform
Reagents
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 10 x 96 tests 5 x 96 tests Mouse IP-10 Capture Antibody 2 x 50µg 1 x 50µg Mouse IP-10 Detector Antibody 2 x 12.5µg 1 x 12.5µg Mouse IP-10 Lyophilized Protein 2 vials 1 vial -
Research areas
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Function
Chemotactic for monocytes and T-lymphocytes. Binds to CXCR3. -
Sequence similarities
Belongs to the intercrine alpha (chemokine CxC) family. -
Post-translational
modificationsCXCL10(1-73) is produced by proteolytic cleavage after secretion from keratinocytes. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- Interferon gamma induced factor MOB1, mouse, homolog of
- Interferon gamma induced protein 10
- 10 kDa interferon gamma induced protein
see all -
Database links
- Entrez Gene: 15945 Mouse
- SwissProt: P17515 Mouse
- Unigene: 877 Mouse
Associated products
Applications
Our Abpromise guarantee covers the use of ab275364 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IA | Use at an assay dependent concentration. | |
| ELISA | Use at an assay dependent concentration. |
Images
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Mouse IP-10 standard curveStandard calibration curve. Background subtracted values are graphed.
References (0)
ab275364 has not yet been referenced specifically in any publications.
