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Immunology Innate Immunity Chemokines Alpha Chemokines (CXC)
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Mouse IP-10 Matched Antibody Pair Kit (ab275364)

  • Datasheet
  • SDS
  • Protocol Booklet
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Mouse IP-10 standard curve

    Key features and details

    • Unlabeled capture antibody, biotin-labeled detection antibody and calibrated protein standard
    • For economical ELISA and ELISA-based assay development
    • Reacts with: Mouse
    • Range: 7.8 pg/ml - 500 pg/ml

    You may also be interested in

    Protein
    Product image
    Recombinant mouse IP10 protein (Active) (ab240846)

    View more associated products

    Overview

    • Product name

      Mouse IP-10 Matched Antibody Pair Kit
      See all IP10 kits
    • Detection method

      Colorimetric
    • Assay type

      ELISA set
    • Sensitivity

      0.168 pg/ml
    • Range

      7.8 pg/ml - 500 pg/ml
    • Species reactivity

      Reacts with: Mouse
    • Product overview

      Mouse IP-10 Matched Antibody Pair Kits include a capture and a biotinylated detector antibody pair, along with a calibrated protein standard, suitable for sandwich ELISA. The Matched Antibody Pair Kit can be used to quantify native and recombinant mouse IP-10.


      Optimization of the kit reagents to sample type, immunoassay format or instrumentation may be required. Guidelines for use of this kit in a standard 96-well microplate sandwich ELISA using HRP/TMB system of colorimetric detection is described in this assay procedure for the purposes of quantification.


      Protocol information and tips on the use of the Matched Antibody Pair kits for sandwich ELISA can be found on our website. An accessory pack can be purchased which includes buffer reagents required to perform 10 x 96-well plate sandwich ELISAs (ab210905).


      For additional information on the performance of the antibody pair used in this kit, please see our equivalent SimpleStep ELISA kit ab260067. Please note that while the antibody pair is the same provided in the corresponding SimpleStep ELISA Kit, due to differences in their formulation, this antibody pair cannot be used with the consumables provided with our SimpleStep ELISA Kits.

    • Tested applications

      Suitable for: IA, ELISAmore details
    • Platform

      Reagents

    Properties

    • Storage instructions

      Store at -20°C. Please refer to protocols.
    • Components 10 x 96 tests 5 x 96 tests
      Mouse IP-10 Capture Antibody 2 x 50µg 1 x 50µg
      Mouse IP-10 Detector Antibody 2 x 12.5µg 1 x 12.5µg
      Mouse IP-10 Lyophilized Protein 2 vials 1 vial
    • Research areas

      • Immunology
      • Innate Immunity
      • Chemokines
      • Alpha Chemokines (CXC)
      • Cancer
      • Invasion/microenvironment
      • Angiogenesis
      • Angiogenic growth factors
      • Kits/ Lysates/ Other
      • Kits
      • ELISA Kits
      • ELISA Kits
      • Cytokines and cytokine receptors ELISA kits
      • Kits/ Lysates/ Other
      • Kits
      • ELISA Kits
      • ELISA Kits
      • Growth factors and hormones ELISA kits
    • Function

      Chemotactic for monocytes and T-lymphocytes. Binds to CXCR3.
    • Sequence similarities

      Belongs to the intercrine alpha (chemokine CxC) family.
    • Post-translational
      modifications

      CXCL10(1-73) is produced by proteolytic cleavage after secretion from keratinocytes.
    • Cellular localization

      Secreted.
    • Target information above from: UniProt accession P02778 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Alternative names

      • Interferon gamma induced factor MOB1, mouse, homolog of
      • Interferon gamma induced protein 10
      • 10 kDa interferon gamma induced protein
      • 10 kDa interferon gamma-induced protein
      • C X C motif chemokine 10
      • C7
      • Chemokine (C X C motif) ligand 10
      • Chemokine CXC motif ligand 10
      • Crg 2
      • CRG2
      • CXCL10
      • CXCL10(1-73)
      • CXL10_HUMAN
      • Gamma IP10
      • Gamma-IP10
      • gIP 10
      • GIP10
      • IFI10
      • INP 10
      • INP10
      • Interferon activated gene 10
      • Interferon gamma induced cell line
      • Interferon inducible cytokine IP 10
      • Interferon inducible cytokine IP10
      • IP 10
      • IP-10
      • Mob 1
      • MOB1
      • Protein 10 from interferon (gamma) induced cell line
      • SCYB10
      • Small inducible cytokine B10
      • Small inducible cytokine B10 precursor
      • Small inducible cytokine subfamily B (Cys X Cys) member 10
      • Small inducible cytokine subfamily B CXC member 10
      • Small inducible cytokine subfamily B, member 10
      • Small-inducible cytokine B10
      see all
    • Database links

      • Entrez Gene: 15945 Mouse
      • SwissProt: P17515 Mouse

      Associated products

        Applications

        The Abpromise guarantee

        Our Abpromise guarantee covers the use of ab275364 in the following tested applications.

        The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

        Application Abreviews Notes
        IA
        Use at an assay dependent concentration.
        ELISA
        Use at an assay dependent concentration.
        Notes
        IA
        Use at an assay dependent concentration.
        ELISA
        Use at an assay dependent concentration.

        Images

        • Mouse IP-10 standard curve
          Mouse IP-10 standard curve

          Standard calibration curve. Background subtracted values are graphed.

        Protocols

        • Protocol Booklet

        Click here to view the general protocols

        Datasheets and documents

        • SDS download

        • Datasheet download

          Download

        References (0)

        Publishing research using ab275364? Please let us know so that we can cite the reference in this datasheet.

        ab275364 has not yet been referenced specifically in any publications.

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