Overview

  • Product name

    Mouse Isotyping Lateral Flow Kit
  • Sample type

    Cell culture supernatant, Ascites Fluid, Purified protein
  • Assay type

    Sandwich (qualitative)
  • Assay time

    0h 10m
  • Assay duration

    One step assay
  • Product overview

    Abcam’s one-step immunochromatographic mouse isotyping kit allows for quick and easy identification of mouse isotypes, subtypes and light chains from a single sample. The sample used can be purified antibody, cell culture supernatant or ascites fluid. For purified antibody and cell culture supernatant the test can also be used to determine the relative purity of a hybridoma cell line. This product is for research use only, not for use in diagnostic applications.

  • Notes

    Lateral-flow membranes are impregnated with separate lines containing antibodies specific for unique amino acid sequences contained within IgG1, IgG2a, IgG2b, IgG3, IgA, IgM, kappa light chains and lambda light chains. When the isotyping strips are added to a test tube containing a diluted mouse immunoglobulin sample, colloidal gold nanoparticles conjugated with antibody form a soluble immune-complex. The complex is apparent in the form of a red band which allows users to make a qualitative determination for their specific sample.

     

  • Tested applications

    Suitable for: Othermore details
  • Platform

    Flow

Properties

Applications

Our Abpromise guarantee covers the use of ab190514 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Other Use at an assay dependent concentration.

Sandwich ELISA (qualitative)

Images

Protocols

References

ab190514 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Question
Answer

Secreted antibody may be IgE, IgD or IgG2c

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Question
Answer

Antibody concentration of sample is too low or hybridoma is not secreting Ig. Re-test the sample at a higher concentration

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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