Product nameMouse Isotyping Lateral Flow Kit
Sample typeCell culture supernatant, Ascites Fluid, Purified protein
Assay typeSandwich (qualitative)
Assay time0h 10m
Assay durationOne step assay
Abcam’s one-step immunochromatographic mouse isotyping kit allows for quick and easy identification of mouse isotypes, subtypes and light chains from a single sample. The sample used can be purified antibody, cell culture supernatant or ascites fluid. For purified antibody and cell culture supernatant the test can also be used to determine the relative purity of a hybridoma cell line. This product is for research use only, not for use in diagnostic applications.
Lateral-flow membranes are impregnated with separate lines containing antibodies specific for unique amino acid sequences contained within IgG1, IgG2a, IgG2b, IgG3, IgA, IgM, kappa light chains and lambda light chains. When the isotyping strips are added to a test tube containing a diluted mouse immunoglobulin sample, colloidal gold nanoparticles conjugated with antibody form a soluble immune-complex. The complex is apparent in the form of a red band which allows users to make a qualitative determination for their specific sample.
Tested applicationsSuitable for: Othermore details
Storage instructionsStore at room temperature. Please refer to protocols.
Components 20 tests Isotyping Mouse Antibody coated test strips (Blue) 1 unit Isotyping Mouse Antibody coated test strips (Red) 1 unit Isotyping Mouse Sample Diluent Buffer 1 x 15ml
Cellular localizationKappa light chain: Cytoplasmic and Secreted Lambda Light chain: Cytoplasmic IgA: Secreted IgM: Isoform 1: Secreted. During differentiation, B lymphocytes switch from expression of membrane bound IgM to secretion of IgM. Isoform 2: Cell membrane; Single pass type I membrane protein. IgG3: Secreted IgG2a: Secreted IgG1: Secreted. Cell membrane; Single-pass membrane protein IgG2b: Secreted
Our Abpromise guarantee covers the use of ab190514 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Other||Use at an assay dependent concentration.
Sandwich ELISA (qualitative)
ab190514 has not yet been referenced specifically in any publications.