Overview

  • Product name

    Mouse L-Selectin ELISA Kit (CD62L)
    See all CD62L kits
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Overall < 10%
    Inter-assay
    Sample n Mean SD CV%
    Overall < 12%
  • Sample type

    Cell culture supernatant, Serum, Plasma
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    < 35 pg/ml
  • Range

    40.96 pg/ml - 10000 pg/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Serum 103.1 95% - 111%
    Plasma 95.69 82% - 104%
    Cell culture media 109.4 103% - 116%

  • Assay duration

    Multiple steps standard assay
  • Species reactivity

    Reacts with: Mouse
  • Product overview

    Abcam's L-Selectin (CD62L) Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay designed for the quantitative measurement of mouse L-Selectin in serum, plasma and cell culture supernatants.


    This assay employs an antibody specific for mouse L-Selectin (CD62L) coated on a 96-well plate. Standards and samples are pipetted into the wells and L-Selectin present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse L-Selectin antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of L-Selectin bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.


    Get higher sensitivity in only 90 minutes with Mouse L-Selectin ELISA Kit (CD62L) (ab199085) from our SimpleStep ELISA® range.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Microplate

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab155448 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • Representative standard curve using ab155448

Protocols

References

ab155448 has not yet been referenced specifically in any publications.

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