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Mouse L-Selectin ELISA Kit (CD62L) (ab155448)

  • Datasheet
  • SDS
  • Protocol Booklet
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L-Selectin Mouse ELISA Kit

    Key features and details

    • Sensitivity: 35 pg/ml
    • Range: 40.96 pg/ml - 10000 pg/ml
    • Sample type: Cell culture supernatant, Plasma, Serum
    • Detection method: Colorimetric
    • Assay type: Sandwich (quantitative)
    • Reacts with: Mouse

    You may also be interested in

    Protein
    Recombinant Mouse CD62L protein (ab194057)

    View more associated products

    Overview

    • Product name

      Mouse L-Selectin ELISA Kit (CD62L)
      See all CD62L kits
    • Detection method

      Colorimetric
    • Precision

      Intra-assay
      Sample n Mean SD CV%
      Overall < 10%
      Inter-assay
      Sample n Mean SD CV%
      Overall < 12%
    • Sample type

      Cell culture supernatant, Serum, Plasma
    • Assay type

      Sandwich (quantitative)
    • Sensitivity

      < 35 pg/ml
    • Range

      40.96 pg/ml - 10000 pg/ml
    • Recovery

      Sample specific recovery
      Sample type Average % Range
      Serum 103.1 95% - 111%
      Plasma 95.69 82% - 104%
      Cell culture media 109.4 103% - 116%
    • Assay duration

      Multiple steps standard assay
    • Species reactivity

      Reacts with: Mouse
    • Product overview

      Abcam's L-Selectin (CD62L) Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay designed for the quantitative measurement of mouse L-Selectin in serum, plasma and cell culture supernatants.


      This assay employs an antibody specific for mouse L-Selectin (CD62L) coated on a 96-well plate. Standards and samples are pipetted into the wells and L-Selectin present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse L-Selectin antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of L-Selectin bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.


      Get higher sensitivity in only 90 minutes with Mouse L-Selectin ELISA Kit (CD62L) (ab199085) from our SimpleStep ELISA® range.

    • Platform

      Microplate

    Properties

    • Storage instructions

      Store at -20°C. Please refer to protocols.
    • Components 1 x 96 tests
      20X Wash Buffer Concentrate 1 x 25ml
      5X Assay Diluent B 1 x 15ml
      700X HRP-Streptavidin Concentrate 1 x 200µl
      Assay Diluent A 1 x 30ml
      Biotinylated anti-mouse L-Selectin 2 vials
      L-Selectin Microplate (12 x 8 wells) 1 unit
      Recombinant Mouse L-Selectin Standard (lyophilized) 2 vials
      Stop Solution 1 x 8ml
      TMB One-Step Substrate Reagent 1 x 12ml
    • Research areas

      • Cardiovascular
      • Blood
      • Other
      • Immunology
      • Adaptive Immunity
      • T Cells
      • CD
      • Signal Transduction
      • Cytoskeleton / ECM
      • Cell Adhesion
      • Cell Adhesion Molecules
      • Endothelial
      • Stem Cells
      • Hematopoietic Progenitors
      • Lymphoid
      • T Lymphocytic Lineage
      • Stem Cells
      • Hematopoietic Progenitors
      • Myeloid
      • Neutrophil Lineage
      • Immunology
      • Adaptive Immunity
      • Regulatory T Cells
      • Kits/ Lysates/ Other
      • Kits
      • ELISA Kits
      • ELISA Kits
      • CD markers ELISA kits
      • Kits/ Lysates/ Other
      • Kits
      • ELISA Kits
      • ELISA Kits
      • Adaptive immune components ELISA kits
    • Relevance

      CD62L is a cell surface component that is a member of a family of adhesion/homing receptors. These play important roles in leukocyte-endothelial cell interactions. CD62L is composed of multiple domains: one homologous to lectins, one to epidermal growth factor, and two to the consensus repeat units found in C3/C4 binding proteins.
    • Cellular localization

      Cell Membrane; single-pass type I membrane protein.
    • Alternative names

      • A.11
      • AI528707
      • CD62 antigen ligand
      • CD62 antigen-like family member L
      • CD62L antigen
      • gp90 MEL
      • hLHRc
      • IgA nephropathy, susceptibility to, included
      • L Selectin
      • L selectin precursor
      • LAM 1
      • LAM1
      • LECAM1
      • Leu 8
      • Leu8
      • Leukocyte adhesion molecule 1
      • Leukocyte endothelial cell adhesion molecule 1
      • Leukocyte surface antigen Leu 8
      • Leukocyte surface antigen Leu8
      • Leukocyte-endothelial cell adhesion molecule 1
      • LNHR
      • LSEL
      • Ly-22
      • Ly-m22
      • Lyam 1
      • LYAM1
      • Lymph node homing receptor
      • Lymphocyte adhesion molecule 1
      • Lymphocyte antigen 22
      • Lymphocyte surface MEL-14 antigen
      • PLNHR
      • Selectin L
      • Selectin L (lymphocyte adhesion molecule 1)
      • Selectin, lymphocyte
      • SELL
      • TQ1
      see all
    • Database links

      • Entrez Gene: 20343 Mouse
      • SwissProt: P18337 Mouse

      Associated products

      • SimpleStep ELISA kits

        • Mouse L-Selectin ELISA Kit (CD62L) (ab199085)

      Images

      • L-Selectin Mouse ELISA Kit
        L-Selectin Mouse ELISA Kit

        Representative standard curve using ab155448

      Protocols

      • Protocol Booklet

      Click here to view the general protocols

      Datasheets and documents

      • SDS download

      • Datasheet download

        Download

      References (1)

      Publishing research using ab155448? Please let us know so that we can cite the reference in this datasheet.

      ab155448 has been referenced in 1 publication.

      • Zimmer DP  et al. Olinciguat, an Oral sGC Stimulator, Exhibits Diverse Pharmacology Across Preclinical Models of Cardiovascular, Metabolic, Renal, and Inflammatory Disease. Front Pharmacol 11:419 (2020). PubMed: 32322204

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