Mouse L-Selectin ELISA Kit (CD62L) (ab155448)

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L-Selectin Mouse ELISA Kit

    Key features and details

    • Sensitivity: 35 pg/ml
    • Range: 40.96 pg/ml - 10000 pg/ml
    • Sample type: Cell culture supernatant, Plasma, Serum
    • Detection method: Colorimetric
    • Assay type: Sandwich (quantitative)
    • Reacts with: Mouse

    Overview

    • Product name

      Mouse L-Selectin ELISA Kit (CD62L)
      See all CD62L kits

    • Detection method

      Colorimetric

    • Precision

      Intra-assay
      Sample n Mean SD CV%
      Overall < 10%
      Inter-assay
      Sample n Mean SD CV%
      Overall < 12%

    • Sample type

      Cell culture supernatant, Serum, Plasma

    • Assay type

      Sandwich (quantitative)

    • Sensitivity

      < 35 pg/ml

    • Range

      40.96 pg/ml - 10000 pg/ml

    • Recovery

      Sample specific recovery
      Sample type Average % Range
      Serum 103.1 95% - 111%
      Plasma 95.69 82% - 104%
      Cell culture media 109.4 103% - 116%

    • Assay duration

      Multiple steps standard assay

    • Species reactivity

      Reacts with: Mouse

    • Product overview

      Abcam's L-Selectin (CD62L) Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay designed for the quantitative measurement of mouse L-Selectin in serum, plasma and cell culture supernatants.


      This assay employs an antibody specific for mouse L-Selectin (CD62L) coated on a 96-well plate. Standards and samples are pipetted into the wells and L-Selectin present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse L-Selectin antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of L-Selectin bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.


      Get higher sensitivity in only 90 minutes with Mouse L-Selectin ELISA Kit (CD62L) (ab199085) from our SimpleStep ELISA® range.

    • Platform

      Microplate

    Properties

    Associated products

    Images

    • L-Selectin Mouse ELISA Kit
      L-Selectin Mouse ELISA Kit

      Representative standard curve using ab155448

    References (1)

    Publishing research using ab155448? Please let us know so that we can cite the reference in this datasheet.

    ab155448 has been referenced in 1 publication.

    • Zimmer DP  et al. Olinciguat, an Oral sGC Stimulator, Exhibits Diverse Pharmacology Across Preclinical Models of Cardiovascular, Metabolic, Renal, and Inflammatory Disease. Front Pharmacol 11:419 (2020). PubMed: 32322204

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