Mouse L-Selectin ELISA Kit (CD62L) (ab155448)
- Datasheet
- Specific References
- Protocols
Overview
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Product nameMouse L-Selectin ELISA Kit (CD62L)
See all CD62L kits -
Detection methodColorimetric
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Precision
Intra-assay Sample n Mean SD CV% Overall < 10% Inter-assay Sample n Mean SD CV% Overall < 12% -
Sample typeCell culture supernatant, Serum, Plasma
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Assay typeSandwich (quantitative)
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Sensitivity< 35 pg/ml
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Range40.96 pg/ml - 10000 pg/ml
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Recovery
Sample specific recovery Sample type Average % Range Serum 103.1 95% - 111% Plasma 95.69 82% - 104% Cell culture media 109.4 103% - 116% -
Assay durationMultiple steps standard assay
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Species reactivityReacts with: Mouse
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Product overview
Abcam's L-Selectin (CD62L) Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay designed for the quantitative measurement of mouse L-Selectin in serum, plasma and cell culture supernatants.
This assay employs an antibody specific for mouse L-Selectin (CD62L) coated on a 96-well plate. Standards and samples are pipetted into the wells and L-Selectin present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse L-Selectin antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of L-Selectin bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Get higher sensitivity in only 90 minutes with Mouse L-Selectin ELISA Kit (CD62L) (ab199085) from our SimpleStep ELISA® range.
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Tested applicationsSuitable for: Sandwich ELISAmore details
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PlatformMicroplate
Properties
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Storage instructionsStore at -20°C. Please refer to protocols.
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Components 1 x 96 tests 20X Wash Buffer Concentrate 1 x 25ml 5X Assay Diluent B 1 x 15ml 700X HRP-Streptavidin Concentrate 1 x 200µl Assay Diluent A 1 x 30ml Biotinylated anti-mouse L-Selectin 2 vials L-Selectin Microplate (12 x 8 wells) 1 unit Recombinant Mouse L-Selectin Standard (lyophilized) 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml -
Research areas
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RelevanceCD62L is a cell surface component that is a member of a family of adhesion/homing receptors. These play important roles in leukocyte-endothelial cell interactions. CD62L is composed of multiple domains: one homologous to lectins, one to epidermal growth factor, and two to the consensus repeat units found in C3/C4 binding proteins.
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Cellular localizationCell Membrane; single-pass type I membrane protein.
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Alternative names
- A.11
- AI528707
- CD62 antigen ligand
see all -
Database links
- Entrez Gene: 20343 Mouse
- SwissProt: P18337 Mouse
Associated products
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SimpleStep ELISA kits
Applications
Our Abpromise guarantee covers the use of ab155448 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Sandwich ELISA | Use at an assay dependent concentration. |
Images
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L-Selectin Mouse ELISA KitRepresentative standard curve using ab155448
Protocols
References
ab155448 has not yet been referenced specifically in any publications.
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"