Overview

  • Product name

    Mouse L-Selectin ELISA Kit (CD62L)
    See all CD62L kits
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Serum 8 7.56%
    Inter-assay
    Sample n Mean SD CV%
    Serum 3 6.55%
  • Sample type

    Cell culture supernatant, Serum, Cell culture extracts, Citrate Plasma
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    1.07 pg/ml
  • Range

    15.6 pg/ml - 1000 pg/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Serum 96 95% - 97%
    Cell culture media 96 93% - 102%
    Citrate Plasma 97 95% - 99%

  • Assay time

    1h 30m
  • Assay duration

    One step assay
  • Species reactivity

    Reacts with: Mouse
    Does not react with: Rat, Rabbit, Goat, Guinea pig, Hamster, Cow, Dog, Human, Pig
  • Product overview

    Abcam’s L-Selectin (CD62L) in vitro SimpleStep ELISA® (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of L-Selectin protein in mouse serum, plasma (citrate), cell culture supernatants and cell extracts
    The SimpleStep ELISA® employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material. TMB substrate is added and during incubation is catalyzed by HRP, generating blue coloration. This reaction is then stopped by addition of Stop Solution completing any color change from blue to yellow. Signal is generated proportionally to the amount of bound analyte and the intensity is measured at 450 nm. Optionally, instead of the endpoint reading, development of TMB can be recorded kinetically at 600 nm.

    Sensitivity:
    Samples diluted in Sample Diluent NS = 1.07 pg/mL
    Samples diluted in 1X Cell Extraction Buffer PTR = 5.53 pg/mL

  • Notes

    L-Selectin is a cell surface glycoprotein and a member of a family of adhesion proteins. L-Selectin was first identified in mice as the Mel-14 antigen, a lymph node homing receptor also found on neutrophils and monocytes expressed on a variety of leukocytes. This molecule acts as adhesion and homing receptor that plays an important role in lymphocyte-endothelial cell recognition and migration. The molecule itself is composed of multiple domains: 1 homologous to lectins, 1 to epidermal growth factor, and 2 to the consensus repeat units found in C3/C4 binding proteins.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Microplate (12 x 8 well strips)

Properties

  • Storage instructions

    Store at +4°C. Please refer to protocols.
  • Components 1 x 96 tests
    10X Mouse L-Selectin Capture Antibody 1 x 600µl
    10X Wash Buffer PT (ab206977) 1 x 20ml
    50X Cell Extraction Enhancer Solution (ab193971) 1 x 1ml
    5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml
    Antibody Diluent 5B 1 x 6ml
    Mouse L-Selectin Detector Antibody (lyophilized) 1 vial
    Mouse L-Selectin Lyophilized Recombinant Protein 2 vials
    Plate Seals 1 unit
    Sample Diluent NS (ab193972) 1 x 50ml
    SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit
    Stop Solution 1 x 12ml
    TMB Development Solution 1 x 12ml
  • Research areas

  • Relevance

    CD62L is a cell surface component that is a member of a family of adhesion/homing receptors. These play important roles in leukocyte-endothelial cell interactions. CD62L is composed of multiple domains: one homologous to lectins, one to epidermal growth factor, and two to the consensus repeat units found in C3/C4 binding proteins.
  • Cellular localization

    Cell Membrane; single-pass type I membrane protein.
  • Alternative names

    • A.11
    • AI528707
    • CD62 antigen ligand
    • CD62 antigen-like family member L
    • CD62L antigen
    • gp90 MEL
    • hLHRc
    • IgA nephropathy, susceptibility to, included
    • L Selectin
    • L selectin precursor
    • LAM 1
    • LAM1
    • LECAM1
    • Leu 8
    • Leu8
    • Leukocyte adhesion molecule 1
    • Leukocyte endothelial cell adhesion molecule 1
    • Leukocyte surface antigen Leu 8
    • Leukocyte surface antigen Leu8
    • Leukocyte-endothelial cell adhesion molecule 1
    • LNHR
    • LSEL
    • Ly-22
    • Ly-m22
    • Lyam 1
    • LYAM1
    • Lymph node homing receptor
    • Lymphocyte adhesion molecule 1
    • Lymphocyte antigen 22
    • Lymphocyte surface MEL-14 antigen
    • PLNHR
    • Selectin L
    • Selectin L (lymphocyte adhesion molecule 1)
    • Selectin, lymphocyte
    • SELL
    • TQ1
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab199085 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

     

  • Background-subtracted data values (mean +/- SD) are graphed.

  • Background-subtracted data values (mean +/- SD) are graphed.

  • Titration of mouse serum and citrate plasma within the working range of the assay. The 1X dilution for mouse serum and plasma was 0.1% matrix. The interpolated L-Selectin concentrations multiplied by the total dilution factor are shown for each matrix (mean +/- SD, n = 2).

  • The 1X dilution for both the stimulated and unstimulated supernatants was 100% matrix. The interpolated L-Selectin concentrations multiplied by the total dilution factor are shown for each matrix (mean +/- SD, n = 2).

Protocols

References

ab199085 has not yet been referenced specifically in any publications.

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