Product nameMouse Legumain Matched Antibody Pair Kit
See all Legumain kits
Assay typeELISA set
Range125 pg/ml - 8000 pg/ml
Species reactivityReacts with: Mouse
Matched Antibody Pair kit is a titrated unlabeled capture antibody, a titrated biotin-labeled detector and a calibrated protein standard. The Matched Antibody Pair Kit can be used to quantify native and recombinant mouse Legumain.
Both capture and detector antibodies are rabbit monoclonal antibodies.
Optimization of the kit reagents to sample type, immunoassay format or instrumentation may be required. Guidelines for use of this kit in a standard 96-well microplate sandwich ELISA using HRP/TMB system of colorimetric detection is described in this assay procedure for the purposes of quantification.
Additional protocol information and tips on the use of the Matched Antibody Pair kits for sandwich ELISA can be found on our website.
For additional information on the performance of the antibody pair used in this kit, please see our equivalent SimpleStep ELISA® (ab207617) which uses the same antibody pair.
Buffer information: The capture antibody is glycerol free. The detector antibody contains glycerol.
To receive an electronic copy of the Certificate of Analysis, please send an email with "CoA for matched antibody pair kit" in the subject line and the desired product number and lot number in the body of the email.
Tested applicationsSuitable for: ELISAmore details
Storage instructionsStore at -20°C. Please refer to protocols.
Components 10 x 96 tests 2 x 96 tests Mouse Legumain Capture Antibody 1 x 100µg 1 x 20µg Mouse Legumain Detector Antibody 1 x 25µg 1 x 5µg Mouse Legumain Lyophilized Protein 1 vial 1 vial
FunctionHas a strict specificity for hydrolysis of asparaginyl bonds. Can also cleave aspartyl bonds slowly, especially under acidic conditions. May be involved in the processing of proteins for MHC class II antigen presentation in the lysosomal/endosomal system.
Tissue specificityUbiquitous. Particularly abundant in kidney, heart and placenta.
Sequence similaritiesBelongs to the peptidase C13 family.
- Information by UniProt
- Asparaginyl endopeptidase
- cysteine 1
SimpleStep ELISA kits
Our Abpromise guarantee covers the use of ab216790 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ELISA||Use at an assay dependent concentration.|
ab216790 has not yet been referenced specifically in any publications.