Overview

  • Product name
    Mouse Leptin ELISA Kit
    See all Leptin kits
  • Detection method
    Colorimetric
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay type
    Sandwich (quantitative)
  • Sensitivity
    < 4 pg/ml
  • Range
    4.1 pg/ml - 1000 pg/ml
  • Recovery

    94 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 94.47 83% - 103%
    Serum 93.29 82% - 102%
    Plasma 95.38 84% - 104%

  • Assay duration
    Multiple steps standard assay
  • Species reactivity
    Reacts with: Mouse
  • Product overview

    Abcam’s Leptin Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Mouse Leptin in serum, plasma and cell culture supernatants.

    This assay employs an antibody specific for Mouse Leptin coated on a 96- well plate. Standards and samples are pipetted into the wells and Leptin present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Mouse Leptin antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Leptin bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • Tested applications
    Suitable for: Sandwich ELISAmore details
  • Platform
    Microplate

Properties

Applications

Our Abpromise guarantee covers the use of ab100718 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • ab100718 Leptin Mouse ELISA Kit
    ab100718 Leptin Mouse ELISA Kit

    Ms Leptin measured in biological fluids showing quantity (pg) per mL of tested sample

  • ab100718 Leptin Mouse ELISA Kit
    ab100718 Leptin Mouse ELISA Kit

    Ms Leptin measured in cell lysates showing quantity (pg) per mg protein

  • Typical Standard Curve
    Typical Standard Curve

    Representative Standard Curve using ab100718

  • Typical Standard Curve
    Typical Standard Curve

    Representative Standard Curve using ab100718

Protocols

References

This product has been referenced in:
  • Losada-Barragán M  et al. Protein malnutrition promotes dysregulation of molecules involved in T cell migration in the thymus of mice infected with Leishmania infantum. Sci Rep 7:45991 (2017). ELISA ; Mouse . Read more (PubMed: 28397794) »
  • Nuñez-Durán E  et al. Protein kinase STK25 aggravates the severity of non-alcoholic fatty pancreas disease in mice. J Endocrinol 234:15-27 (2017). ELISA . Read more (PubMed: 28442507) »

See all 6 Publications for this product

Publishing research using ab100718? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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Protocol for cell lysate (lysis buffer and protocol recommendations)

We have not yet tested ab100718 with cell lysates. However, the kit is compatible with many different samples types so lysates will most likely work. If you decide to test this kit with lysate samples, we recommend diluting the samples at least 5-fold ...

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I would like to measure Leptin in either plasma or serum of pigs. I would like to know the chances of one of the abcam available leptin ELISA kits to work in pigs. Find below the pig leptin aminoacid sequence
MRCGPLCRFLWLWPYLSYVEAVPIWRVQDDTKTLIKTI...

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Thank you very much for your inquiry and your interest in our kits.

Unfortunately,the cross-reactivity of ab108879and ab108881 with pig samples is low, around 5%.

For the other three kits, ab100581, ab100718 and ab100773,the laborat...

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What type of graph is required for the results from this kit, ab100718?

Thank you for your enquiry.

I have now received further information regarding the reading of the results from this kit. The manuals advise using a log-log model as this type of plot will fit most data sets very well. However, you can use any...

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Can this kit be used with blood samples? How much of my sample should I start with? Is there a suggested dilution/concentration?

Thank you for your inquiry.  This kit ab100718 has been validated for serum, plasma, and cell culture supernatant samples. Are you using whole blood samples?  If so, this kit may work, but we would not recommend it as the chances of experiencing hi...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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