Key features and details
- Sensitivity: 4 pg/ml
- Range: 4.1 pg/ml - 1000 pg/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
Product nameMouse Leptin ELISA Kit
See all Leptin kits
Sample typeCell culture supernatant, Serum, Plasma
Assay typeSandwich (quantitative)
Sensitivity< 4 pg/ml
Range4.1 pg/ml - 1000 pg/ml
Sample specific recovery Sample type Average % Range Cell culture supernatant 94.47 83% - 103% Serum 93.29 82% - 102% Plasma 95.38 84% - 104%
Assay durationMultiple steps standard assay
Species reactivityReacts with: Mouse
Mouse Leptin ELISA kit is designed for the quantitative measurement of Mouse Leptin in serum, plasma and cell culture supernatants.
This assay employs an antibody specific for Mouse Leptin coated on a 96- well plate. Standards and samples are pipetted into the wells and Leptin present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Mouse Leptin antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Leptin bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Storage instructionsStore at -20°C. Please refer to protocols.
Components 1 x 96 tests 120X HRP-Streptavidin Concentrate 1 x 200µl 20X Wash Buffer 1 x 25ml 5X Assay Diluent B 1 x 15ml Assay Diluent A 1 x 30ml Biotinylated anti-Mouse Leptin 2 vials Leptin Microplate (12 strips x 8 wells) 1 unit Recombinant Mouse Leptin Standard (lyophilized) 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml
FunctionMay function as part of a signaling pathway that acts to regulate the size of the body fat depot. An increase in the level of LEP may act directly or indirectly on the CNS to inhibit food intake and/or regulate energy expenditure as part of a homeostatic mechanism to maintain constancy of the adipose mass.
Involvement in diseaseDefects in LEP may be a cause of obesity (OBESITY) [MIM:601665]. It is a condition characterized by an increase of body weight beyond the limitation of skeletal and physical requirements, as the result of excessive accumulation of body fat.
Sequence similaritiesBelongs to the leptin family.
- Information by UniProt
ab100718 has been referenced in 21 publications.
- Tang Y et al. Rapid responses of adipocytes to iron overload increase serum TG level by decreasing adiponectin. J Cell Physiol N/A:N/A (2021). PubMed: 33855731
- Lee K et al. Dietary Silk Peptide Prevents High-Fat Diet-Induced Obesity and Promotes Adipose Browning by Activating AMP-Activated Protein Kinase in Mice. Nutrients 12:N/A (2020). PubMed: 31941008
- Kim JH et al. The Protective Effects of Acer okamotoanum and Isoquercitrin on Obesity and Amyloidosis in a Mouse Model. Nutrients 12:N/A (2020). PubMed: 32397362
- Wei Z et al. Disordered Leptin signaling in the retrotrapezoid nucleus is associated with the impaired hypercapnic ventilatory response in obesity. Life Sci 257:117994 (2020). PubMed: 32569780
- Colorado D et al. Metabolic Activity of Anthocyanin Extracts Loaded into Non-ionic Niosomes in Diet-Induced Obese Mice. Pharm Res 37:152 (2020). PubMed: 32700034