Overview

  • Product name

    Mouse Leptin ELISA Kit
    See all Leptin kits
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Overall 4.5%
    Inter-assay
    Sample n Mean SD CV%
    Overall 9.1%
  • Sample type

    Cell culture supernatant, Serum, Plasma
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    = 0.3 ng/ml
  • Range

    0.375 ng/ml - 24 ng/ml
  • Assay time

    5h 00m
  • Assay duration

    Multiple steps standard assay
  • Species reactivity

    Reacts with: Mouse
  • Product overview

    Abcam’s Leptin mouse in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of leptin levels in plasma, serum and cell culture supernatants.


    A Leptin specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a Leptin specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Leptin captured in plate.


    The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Microplate

Properties

Applications

Our Abpromise guarantee covers the use of ab108881 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent dilution.

Images

  • Representative Standard Curve using ab108881

Protocols

References

This product has been referenced in:

  • Cui XB  et al. RGC32 deficiency protects against high-fat diet-induced obesity and insulin resistance in mice. J Endocrinol 224:127-37 (2015). Functional Studies ; Mouse . Read more (PubMed: 25385871) »
See 1 Publication for this product

Customer reviews and Q&As

Answer

Thank you very much for your inquiry and your interest in our kits.

Unfortunately,the cross-reactivity of ab108879and ab108881 with pig samples is low, around 5%.

For the other three kits, ab100581, ab100718 and ab100773,the laboratory has not tested these on pig samples and no prediction can be made, as not all epitopes are known.

For the most part we do not see cross-species reactivity with sandwich based assays. If you would however nevertheless like to test on of these kits, please do let me know, as I would be able to provide you with an interesting testing offer.

Please do also let me know whether you would like to discuss other options. Indeed, we have many individual antibodies against leptin in our catalog as well. Some of them are tested in pig (ab3583 and ab16227) and others are tested in ELISA. Again, we could discuss options and provide testing offers if you are interested in this.

The testing offers would be along the line of the offer described here:
https://www.abcam.com/collaborationdiscount

I hope this information is helpful. I am looking forward to hear back from you.

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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