Overview

  • Product name
    Mouse Leptin ELISA Kit
    See all Leptin kits
  • Detection method
    Colorimetric
  • Precision
    Intra-assay
    Sample n Mean SD CV%
    Serum 5 5.9%
    Inter-assay
    Sample n Mean SD CV%
    Serum 3 11.5%
  • Sample type
    Cell culture supernatant, Urine, Serum, Citrate Plasma
  • Assay type
    Sandwich (quantitative)
  • Sensitivity
    33.6 pg/ml
  • Range
    93.75 pg/ml - 6000 pg/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Urine 80 77% - 82%
    Serum 111 110% - 114%
    Cell culture media 108 107% - 108%
    Heparin Plasma 106 94% - 116%
    Citrate Plasma 104 100% - 111%
    serum free media 104 103% - 105%

  • Assay time
    1h 30m
  • Assay duration
    One step assay
  • Species reactivity
    Reacts with: Mouse, Rat
    Does not react with: Rabbit, Goat, Guinea pig, Cow, Dog, Pig
  • Product overview

    Leptin in vitro SimpleStep ELISA® (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Leptin protein in mouse serum, plasma (citrate), urine and cell culture supernatant.


    The SimpleStep ELISA® employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material. TMB substrate is added and during incubation is catalyzed by HRP, generating blue coloration. This reaction is then stopped by addition of Stop Solution completing any color change from blue to yellow. Signal is generated proportionally to the amount of bound analyte and the intensity is measured at 450 nm. Optionally, instead of the endpoint reading, development of TMB can be recorded kinetically at 600 nm.

  • Notes

    Leptin is a secreted protein factor produced by adipocytes. It regulates energy balance and body fat deposits. Leptin deficiency in humans and mice can cause obesity. Circulating levels of Leptin are regulated by food intake, insulin levels and pregnancy status. Mouse leptin has 96% and 85% protein sequence identity to rat and human leptin, respectively.

  • Tested applications
    Suitable for: Sandwich ELISAmore details
  • Platform
    Microplate (12 x 8 well strips)

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab199082 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • Background-subtracted data values (mean +/- SD) are graphed.

  • The concentrations of Leptin were measured in duplicate and interpolated from the Leptin standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Leptin concentration was determined to be 4514.3 pg/mL in mouse serum and 2698.1 pg/mL in mouse plasma (Citrate).

  • The concentrations of Leptin were measured in duplicate and interpolated from the Leptin standard curve and corrected for sample dilution.  The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).  The mean Leptin concentration was determined to be 2983 pg/mL in rat serum, 1626 pg/mL in rat plasma (citrate) and 2134 pg/mL in rat plasma (heparin).

Protocols

References

This product has been referenced in:
  • Matsui S  et al. Neuronal SIRT1 regulates macronutrient-based diet selection through FGF21 and oxytocin signalling in mice. Nat Commun 9:4604 (2018). Read more (PubMed: 30389922) »
See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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