Overview

  • Product name
    Mouse Leptin ELISA Kit, Fluorescent
    See all Leptin kits
  • Detection method
    Fluorescent
  • Precision
    Intra-assay
    Sample n Mean SD CV%
    Mouse serum 5 5.9%
    Inter-assay
    Sample n Mean SD CV%
    Mouse serum 3 11.5%
  • Sample type
    Cell culture supernatant, Urine, Serum, Heparin Plasma, Citrate Plasma
  • Assay type
    Sandwich (quantitative)
  • Sensitivity
    21.4 pg/ml
  • Range
    23.4 pg/ml - 48000 pg/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Urine 80 77% - 82%
    Serum 111 110% - 114%
    Cell culture media 104 103% - 105%
    Heparin Plasma 106 94% - 116%
    Citrate Plasma 104 100% - 111%

  • Assay time
    1h 30m
  • Assay duration
    One step assay
  • Species reactivity
    Reacts with: Mouse, Rat
    Does not react with: Rabbit, Goat, Guinea pig, Cow, Dog, Pig
  • Product overview

    Leptin in vitro CatchPoint SimpleStep ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Leptin protein in mouse serum, plasma (citrate), urine and cell culture supernatants.


    This CatchPoint SimpleStep ELISA kit has been optimized for Molecular Devices Microplate Readers. Click here for a list of recommended Microplate Readers.
    If using a Molecular Devices’ plate reader supported by SoftMax® Pro software, a preconfigured protocol for these CatchPoint SimpleStep ELISA Kits is available with all the protocol and analysis settings at www.softmaxpro.org.


    The CatchPoint SimpleStep ELISA employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material. CatchPoint HRP Development Solution containing the Stoplight Red Substrate is added. During incubation, the substrate is catalyzed by HRP generating a fluorescent product. Signal is generated proportionally to the amount of bound analyte and the intensity is measured in a fluorescence plater reader at 530/570/590 nm Excitation/Cutoff/Emission.

  • Notes

    Leptin is a secreted protein factor produced by adipocytes. It regulates energy balance and body fat deposits. Leptin deficiency in humans and mice can cause obesity. Circulating levels of Leptin are regulated by food intake, insulin levels and pregnancy status. Mouse leptin has 96% and 85% protein sequence identity to rat and human leptin, respectively.

  • Tested applications
    Suitable for: Sandwich ELISAmore details
  • Platform
    Pre-coated microplate (12 x 8 well strips)

Properties

Applications

Our Abpromise guarantee covers the use of ab229421 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • ELISA Protocol Summary
  • Background-subtracted data values (mean +/- SD) are graphed.

  • The concentrations of Leptin were measured in duplicate and interpolated from the Leptin standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Leptin concentration was determined to be 4514.3 pg/mL in mouse serum and 2698.1 pg/mL in mouse plasma (Citrate).

  • The concentrations of Leptin were measured in duplicate and interpolated from the Leptin standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Leptin concentration was determined to be 2983 pg/mL in rat serum, 1626 pg/mL in rat plasma (citrate) and 2134 pg/mL in rat plasma (heparin).

Protocols

References

ab229421 has not yet been referenced specifically in any publications.

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