Overview

  • Product name

    Mouse LH Beta ELISA Kit
    See all LHB kits
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Pituitary 8 4.9%
  • Sample type

    Tissue Extracts
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    4.7 pg/ml
  • Range

    117 pg/ml - 7500 pg/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Tissue Extracts 97 % - %

  • Assay time

    1h 30m
  • Assay duration

    One step assay
  • Species reactivity

    Reacts with: Mouse
  • Product overview

    LH Beta in vitro SimpleStep ELISA® (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of LH Beta protein in mouse tissue extract samples.


    The SimpleStep ELISA® employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material. TMB substrate is added and during incubation is catalyzed by HRP, generating blue coloration. This reaction is then stopped by addition of Stop Solution completing any color change from blue to yellow. Signal is generated proportionally to the amount of bound analyte and the intensity is measured at 450 nm. Optionally, instead of the endpoint reading, development of TMB can be recorded kinetically at 600 nm.


    LH Beta is a polypeptide synthesized by gonadotropic cells. Together with the alpha subunit, they form the heterodimeric luteinizing hormone, which stimulates ovulation and testosterone synthesis. Urinary luteinizing hormone levels are used to predict fertility timing. Dysregulation of this system leads to premature menopause, hypogonadism, and is associated with a host of sexual development disorders.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Pre-coated microplate (12 x 8 well strips)

Properties

  • Storage instructions

    Store at +4°C. Please refer to protocols.
  • Components 1 x 96 tests
    10X Mouse LH Beta Capture Antibody 1 x 600µl
    10X Mouse LH Beta Detector Antibody 1 x 600µl
    10X Wash Buffer PT (ab206977) 1 x 20ml
    50X Cell Extraction Enhancer Solution (ab193971) 1 x 1ml
    5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml
    Antibody Diluent 5BI 1 x 6ml
    Mouse LH Beta Lyophilized Recombinant Protein 2 vials
    Plate Seals 1 unit
    Sample Diluent NS (ab193972) 1 x 12ml
    SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit
    Stop Solution 1 x 12ml
    TMB Development Solution 1 x 12ml

Associated products

Applications

Our Abpromise guarantee covers the use of ab235648 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

     

  • Background-subtracted data values (mean +/- SD) are graphed.

  • The concentrations of LH Beta were measured in duplicate and interpolated from the LH Beta standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean LH Beta concentration was determined to be 1.84 ng/mL in mouse pituitary extract.

Protocols

References

ab235648 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Abreviews
Ab235648 mouse LH beta Elisa kit was tried for LH in media of tissue culture and in mouse serum.
Samples included in trial:
5ul from standard stock (192ng/ml) were added to 110ul of cell culture media.
The same media was used to verify background.
Media from primary gonadotrophs cells was taken 20 minutes after 1 minute treatment with 1nM GnRH (or mock).
Serum was taken from 47 days old female mice at estrus.
All samples included 1x cell extraction enhancer solution. (2.2ul of x50 was added to 110ul sample).
All samples and standards were measured as 50ul duplicates.
Incubation with the antibody mix was for 2 hr at RT.
The rest of the protocol – as in the manual.
Calculation – every well was read 3 times and averaged.
every duplicate was averaged.
standard tube #8 (no protein added) served as blank and was subtracted from each result.
standards up to 938pg/ml were linear and their equation was used for LH concentration calculation.
values above that – were estimated by proximity to the relevant standard.
Under these conditions, LH could be detected in both serum and media.

Dr. Lilach Polinsky

Verified customer

Submitted May 02 2019

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