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Cell Biology Apoptosis Intracellular Associated Proteins
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Mouse Lipocalin-2 ELISA Kit (NGAL) (ab119601)

  • Datasheet
  • SDS
  • Protocol Booklet
Submit a review Q&A (3)References (4)

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ELISA: Lipocalin-2 (NGAL) Mouse ELISA Kit (ab119601)
  • ELISA: Lipocalin-2 (NGAL) Mouse ELISA Kit (ab119601)
  • ELISA: Lipocalin-2 (NGAL) Mouse ELISA Kit (ab119601)
  • Typical Standard Curve

Key features and details

  • Sensitivity: 10 pg/ml
  • Range: 78 pg/ml - 5000 pg/ml
  • Sample type: Cell culture supernatant, EDTA Plasma, Hep Plasma, Serum, Urine
  • Detection method: Colorimetric
  • Assay type: Sandwich (quantitative)
  • Reacts with: Mouse

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Protein
Recombinant Mouse Lipocalin-2 / NGAL protein (ab95008)

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Overview

  • Product name

    Mouse Lipocalin-2 ELISA Kit (NGAL)
    See all Lipocalin-2 / NGAL kits
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    1 16 141pg/ml 6.2 = 4.4%
    2 16 785pg/ml 38.46 = 4.9%
    3 16 2588pg/ml 113.87 = 4.4%
    Inter-assay
    Sample n Mean SD CV%
    1 24 130pg/ml 7.54 = 5.8%
    2 24 817pg/ml 54.73 = 6.7%
    3 24 2606pg/ml 161.57 = 6.2%
  • Sample type

    Cell culture supernatant, Urine, Serum, Hep Plasma, EDTA Plasma
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    < 10 pg/ml
  • Range

    78 pg/ml - 5000 pg/ml
  • Assay duration

    Multiple steps standard assay
  • Species reactivity

    Reacts with: Mouse
  • Product overview

    Abcam’s mouse Lipocalin-2 (NGAL) in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of mouse Lipocalin-2 in cell culture supernatants, serum, plasma (heparin, EDTA) and urine. (Genecards: Lcn2)


    A Lipocalin-2 specific rat monoclonal antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells and incubated. A biotinylated detection polyclonal antibody from goat specific for Lipocalin-2 is then added followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. TMB is then used to visualize the HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the mouse Lipocalin-2 amount of sample captured in plate.


    Get higher sensitivity in only 90 minutes with Mouse Lipocalin-2 ELISA Kit (NGAL) (ab199083) from our SimpleStep ELISA® range.

  • Platform

    Microplate

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components Identifier 1 x 96 tests
    ABC Diluent Buffer Blue Cap 1 x 12ml
    Antibody Diluent Buffer Green Cap 1 x 12ml
    Anti-mouse Lipocalin-2 antibody Microplate (12 x 8 wells) 1 unit
    Avidin-Biotin-Peroxidase Complex (ABC) 1 x 100µl
    Biotinylated anti-mouse Lipocalin-2 antibody 1 x 130µl
    Lyophilized recombinant mouse Lipocalin-2 standard 2 x 10ng
    Plate Seal 4 units
    Sample Diluent Buffer Green Cap 1 x 30ml
    TMB Color Developing Agent Amber Bottle 1 x 10ml
    TMB Stop Solution Yellow Cap 1 x 10ml
  • Research areas

    • Cell Biology
    • Apoptosis
    • Intracellular
    • Associated Proteins
    • Immunology
    • Adaptive Immunity
    • T Cells
    • Non-CD
    • Immunology
    • Innate Immunity
    • Complement
    • Regulatory
    • Cardiovascular
    • Lipids / Lipoproteins
    • Fatty Acids
    • Binding proteins
    • Immunology
    • Innate Immunity
    • Cytokines
    • Other
    • Signal Transduction
    • Metabolism
    • Vitamins / Minerals
    • Signal Transduction
    • Metabolism
    • Lipid metabolism
    • Cancer
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    • Other
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    • Kits
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    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Lipid and lipoprotein metabolism
    • Fatty acids
    • Metabolism
    • Pathways and Processes
    • Cofactors, Vitamins / minerals
    • Vitamins / minerals
    • Metabolism
    • Types of disease
    • Cancer
    • Metabolism
    • Pathways and Processes
    • Redox metabolism
    • Fatty acid oxidation
  • Function

    Iron-trafficking protein involved in multiple processes such as apoptosis, innate immunity and renal development. Binds iron through association with 2,5-dihydroxybenzoic acid (2,5-DHBA), a siderophore that shares structural similarities with bacterial enterobactin, and delivers or removes iron from the cell, depending on the context. Iron-bound form (holo-24p3) is internalized following binding to the SLC22A17 (24p3R) receptor, leading to release of iron and subsequent increase of intracellular iron concentration. In contrast, association of the iron-free form (apo-24p3) with the SLC22A17 (24p3R) receptor is followed by association with an intracellular siderophore, iron chelation and iron transfer to the extracellular medium, thereby reducing intracellular iron concentration. Involved in apoptosis due to interleukin-3 (IL3) deprivation: iron-loaded form increases intracellular iron concentration without promoting apoptosis, while iron-free form decreases intracellular iron levels, inducing expression of the proapoptotic protein BCL2L11/BIM, resulting in apoptosis. Involved in innate immunity, possibly by sequestrating iron, leading to limit bacterial growth.
  • Tissue specificity

    Expressed in bone marrow and in tissues that are prone to exposure to microorganism. High expression is found in bone marrow as well as in uterus, prostate, salivary gland, stomach, appendix, colon, trachea and lung. Not found in the small intestine or peripheral blood leukocytes.
  • Sequence similarities

    Belongs to the calycin superfamily. Lipocalin family.
  • Cellular localization

    Secreted. Upon binding to the SLC22A17 (24p3R) receptor, it is internalized.
  • Target information above from: UniProt accession P80188 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Alternative names

    • 24p3
    • 25 kDa alpha 2 microglobulin related subunit of MMP9
    • 25 kDa alpha-2-microglobulin-related subunit of MMP-9
    • Alpha 2 microglobulin related protein
    • HGNC:6526
    • HNL
    • Lcn 2
    • Lcn2
    • Lipocalin-2
    • Migration stimulating factor inhibitor
    • MSFI
    • Neutrophil gelatinase associated lipocalin
    • Neutrophil gelatinase associated lipocalin precursor
    • Neutrophil gelatinase-associated lipocalin
    • NGAL
    • NGAL_HUMAN
    • Oncogene 24p3
    • Oncogenic lipocalin 24p3
    • p25
    • Siderocalin
    • siderocalin LCN2
    • SV40 induced 24P3 protein
    • Uterocalin
    see all
  • Database links

    • Entrez Gene: 16819 Mouse
    • SwissProt: P11672 Mouse
    • Unigene: 9537 Mouse

    Associated products

    • SimpleStep ELISA kits

      • Mouse Lipocalin-2 ELISA Kit (NGAL) (ab199083)

    Images

    • ELISA: Lipocalin-2 (NGAL) Mouse ELISA Kit (ab119601)
      ELISA: Lipocalin-2 (NGAL) Mouse ELISA Kit (ab119601)

      Lipocalin-2 measured in biological fluids (dilution range 1/50-1/500; duplicates +/- SD).

    • ELISA: Lipocalin-2 (NGAL) Mouse ELISA Kit (ab119601)
      ELISA: Lipocalin-2 (NGAL) Mouse ELISA Kit (ab119601)

      Mouse lipocalin-2 measured in tissue lysates (10-50 ug protein per mL tested; presented as NGAL per mg of extracted protein; duplicates +/- SD).

    • ELISA: Lipocalin-2 (NGAL) Mouse ELISA Kit (ab119601)
      ELISA: Lipocalin-2 (NGAL) Mouse ELISA Kit (ab119601)

      Lipocalin-2 measured in culture supernatants (dilution range 1/1-1/25) from control (-) or activated J774 cells ((PHA) 48 hours with 2% PHA-M; LifeTechnologies) and from RAW264 cells stimulated for 24 hours with 50 ng x mL-1 of PMA (ab120297) with the addition of 1 ug x mL-1 of LPS (Sigma) for the last 6 hours (PMA/LPS) (duplicates +/- SD).

    • Typical Standard Curve
      Typical Standard Curve

      Representative Standard Curve using ab119601.

    Protocols

    • Protocol Booklet

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
    • SDS
  • References (4)

    Publishing research using ab119601? Please let us know so that we can cite the reference in this datasheet.

    ab119601 has been referenced in 4 publications.

    • Gibbs KM  et al. Effects of General Anesthesia on 2 Urinary Biomarkers of Kidney Injury-Hepatitis A Virus Cellular Receptor 1 and Lipocalin 2-in Male C57BL/6J Mice. J Am Assoc Lab Anim Sci 58:21-29 (2019). PubMed: 30538007
    • Zhang L  et al. Effects of Farnesiferol B on Ischemia-Reperfusion-Induced Renal Damage, Inflammation, and NF-?B Signaling. Int J Mol Sci 20:N/A (2019). PubMed: 31842453
    • Jackson EK  et al. Renal 2',3'-Cyclic Nucleotide 3'-Phosphodiesterase Is an Important Determinant of AKI Severity after Ischemia-Reperfusion. J Am Soc Nephrol 27:2069-81 (2016). PubMed: 26574047
    • Vandekerckhove L  et al. Neuregulin-1 attenuates development of nephropathy in a type 1 diabetes mouse model with high cardiovascular risk. Am J Physiol Endocrinol Metab 310:E495-504 (2016). PubMed: 26786778

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-3 of 3 Abreviews or Q&A

    Question

    Customer kindly contacted us to inquire if this product might work in mouse urine samples.

    Read More

    Abcam community

    Verified customer

    Asked on Mar 12 2013

    Answer

    I have been informed by the lab that this product is tested and does work in mouse urine samples. We have added urine to the sample types guaranteed.

    Read More

    Abcam Scientific Support

    Answered on Mar 12 2013

    Question

    As I’m not familiar with these items, I tried to find the ab codes through your site.
    For the items I found a relative code I have update the list.
    For those I don’t have a reference code, this is because either no products matches or all available products reacts for humans when we need them for pigs.
    So please check the list again and advice.
    https://www.abcam.com/Procalcitonin-Human-ELISA-Kit-ab100630.html Porcine Procalcitonin, PCT ELISA kit 96 Tests
    https://www.abcam.com/IL6-Pig-ELISA-Kit-1-x-96-Well-Plate-ab100755.html Porcine IL-6 Quantikine ELISA kit 96 Tests
    https://www.abcam.com/TNF-alpha-Porcine-ELISA-Kit-ab100756.html Porcine TNF-alpha/ TNFSF1A Quantikine ELISA kit 96 Tests
    https://www.abcam.com/Cortisol-Human-ELISA-Kit-ab108674.html Porcine Cortisol ELISA kit 96 Tests ? (Is it the same for humans and pigs?)
    Porcine Noradrenalin ELISA kit 96 Tests (No product match)
    Porcine Adrenaline ELISA kit 96 Tests (No product match)
    https://www.abcam.com/Albumin-Swine-ELISA-Kit-ab108794.html Porcine Serum Cystatin C ELISA Kit 96 Tests
    Porcine Urinary Cystatin C ELISA Kit 96 Tests (Only for humans)
    https://www.abcam.com/IL8-Pig-ELISA-Kit-ab113352.html Porcine Serum NGAL (Neutrophil Gelatinase-Associated Lipocalin) ELISA Kit 96 Tests
    Porcine Urinary NGAL (Neutrophil Gelatinase-Associated Lipocalin) ELISA Kit 96 Tests (Only for humans)
    Porcine Urinary KIM-1 (Kidney Injury Molecule -1) ELISA Kit 96 Tests (Only for humans)
    https://www.abcam.com/IL8-Pig-ELISA-Kit-ab113352.html Porcine Urinary IL-18 (Interleukin -18) ELISA Kit 96 Tests
    Porcine Urinary NAG (N-Acetyl-β-D-Glucosaminidase) ELISA Kit96 Tests (Only for humans)
    https://www.abcam.com/IFN-gamma-Pig-ELISA-Kit-ab113353.html Porcine Urinary L-FABP (Liver-type Fatty-Acid Binding Protein) ELISA Kit 96 Tests

    Read More

    Abcam community

    Verified customer

    Asked on Jun 26 2012

    Answer

    I have received information regarding

    ab119589 Cystatin C Human ELISA Kit
    ab119590 Cystatin C Mouse ELISA Kit
    ab119596 KIM1 Mouse ELISA Kit
    ab119597 KIM1 Rat ELISA Kit
    ab119601 Lipocalin-2 / NGAL Mouse ELISA Kit
    ab119601 Lipocalin-2 / NGAL Mouse ELISA Kit

    these has not been tested with Swine or pig samples so we are unable to confirm the species cross reactivity. It mean only ab100755, ab100756, ab108674, ab108794, ab113352, ab113353 are OK to use with Porcine samples and are guaranteed. Would you like to have a quote for these antibodies.

    I hope this info will be helpful. Should you require more information please do not hesitate to contact me.

    Read More

    Abcam Scientific Support

    Answered on Jun 26 2012

    Question

    As I’m not familiar with these items, I tried to find the ab codes through your site.
    For the items I found a relative code I have update the list.
    For those I don’t have a reference code, this is because either no products matches or all available products reacts for humans when we need them for pigs.
    So please check the list again and advice.
    https://www.abcam.com/Procalcitonin-Human-ELISA-Kit-ab100630.html Porcine Procalcitonin, PCT ELISA kit 96 Tests
    https://www.abcam.com/IL6-Pig-ELISA-Kit-1-x-96-Well-Plate-ab100755.html Porcine IL-6 Quantikine ELISA kit 96 Tests
    https://www.abcam.com/TNF-alpha-Porcine-ELISA-Kit-ab100756.html Porcine TNF-alpha/ TNFSF1A Quantikine ELISA kit 96 Tests
    https://www.abcam.com/Cortisol-Human-ELISA-Kit-ab108674.html Porcine Cortisol ELISA kit 96 Tests ? (Is it the same for humans and pigs?)
    Porcine Noradrenalin ELISA kit 96 Tests (No product match)
    Porcine Adrenaline ELISA kit 96 Tests (No product match)
    https://www.abcam.com/Albumin-Swine-ELISA-Kit-ab108794.html Porcine Serum Cystatin C ELISA Kit 96 Tests
    Porcine Urinary Cystatin C ELISA Kit 96 Tests (Only for humans)
    https://www.abcam.com/IL8-Pig-ELISA-Kit-ab113352.html Porcine Serum NGAL (Neutrophil Gelatinase-Associated Lipocalin) ELISA Kit 96 Tests
    Porcine Urinary NGAL (Neutrophil Gelatinase-Associated Lipocalin) ELISA Kit 96 Tests (Only for humans)
    Porcine Urinary KIM-1 (Kidney Injury Molecule -1) ELISA Kit 96 Tests (Only for humans)
    https://www.abcam.com/IL8-Pig-ELISA-Kit-ab113352.html Porcine Urinary IL-18 (Interleukin -18) ELISA Kit 96 Tests
    Porcine Urinary NAG (N-Acetyl-β-D-Glucosaminidase) ELISA Kit96 Tests (Only for humans)
    https://www.abcam.com/IFN-gamma-Pig-ELISA-Kit-ab113353.html Porcine Urinary L-FABP (Liver-type Fatty-Acid Binding Protein) ELISA Kit 96 Tests

    Read More

    Abcam community

    Verified customer

    Asked on Jun 25 2012

    Answer

    Thank you for contacting us.


    To let you know, we are currently investigating the Porcine (pig) species, cross-reactivity information for following kits ab119602, ab113326, ab119600, ab119601, ab119590 and ab119589. We will send you the details soon.

    The other kits ab100755, ab100756, ab108674, ab108794, ab113352, ab113353 are OK to use with Porcine samples and are guaranteed.

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Use our products? Submit an Abreview. Earn rewards!
    https://www.abcam.com/abreviews

    Read More

    Abcam Scientific Support

    Answered on Jun 25 2012

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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