Mouse Lipocalin-2 ELISA Kit (NGAL) (ab119601)
- Datasheet
- References (2)
- Protocols
Overview
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Product name
Mouse Lipocalin-2 ELISA Kit (NGAL)
See all Lipocalin-2 / NGAL kits -
Detection method
Colorimetric -
Sample type
Cell culture supernatant, Urine, Serum, Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
< 10 pg/ml -
Range
78 pg/ml - 5000 pg/ml -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Mouse -
Product overview
Abcam’s mouse Lipocalin-2 (NGAL) in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of mouse Lipocalin-2 in cell culture supernatants, serum, plasma (heparin) and urine. (Genecards: Lcn2)
A Lipocalin-2 specific rat monoclonal antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells and incubated. A biotinylated detection polyclonal antibody from goat specific for Lipocalin-2 is then added followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. TMB is then used to visualize the HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the mouse Lipocalin-2 amount of sample captured in plate.
Get higher sensitivity in only 90 minutes with Mouse Lipocalin-2 ELISA Kit (NGAL) (ab199083) from our SimpleStep ELISA® range.
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Tested applications
Suitable for: Sandwich ELISAmore details -
Platform
Microplate
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components Identifier 1 x 96 tests ABC Diluent Buffer Blue Cap 1 x 12ml Antibody Diluent Buffer Green Cap 1 x 12ml Anti-mouse Lipocalin-2 antibody Microplate (12 x 8 wells) 1 unit Avidin-Biotin-Peroxidase Complex (ABC) 1 x 130µl Biotinylated anti-mouse Lipocalin-2 antibody 1 x 130µl Lyophilized recombinant mouse Lipocalin-2 standard 2 x 10ng Plate Seal 4 units Sample Diluent Buffer Green Cap 1 x 30ml TMB Color Developing Agent Amber Bottle 1 x 10ml TMB Stop Solution Yellow Cap 1 x 10ml -
Research areas
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Function
Iron-trafficking protein involved in multiple processes such as apoptosis, innate immunity and renal development. Binds iron through association with 2,5-dihydroxybenzoic acid (2,5-DHBA), a siderophore that shares structural similarities with bacterial enterobactin, and delivers or removes iron from the cell, depending on the context. Iron-bound form (holo-24p3) is internalized following binding to the SLC22A17 (24p3R) receptor, leading to release of iron and subsequent increase of intracellular iron concentration. In contrast, association of the iron-free form (apo-24p3) with the SLC22A17 (24p3R) receptor is followed by association with an intracellular siderophore, iron chelation and iron transfer to the extracellular medium, thereby reducing intracellular iron concentration. Involved in apoptosis due to interleukin-3 (IL3) deprivation: iron-loaded form increases intracellular iron concentration without promoting apoptosis, while iron-free form decreases intracellular iron levels, inducing expression of the proapoptotic protein BCL2L11/BIM, resulting in apoptosis. Involved in innate immunity, possibly by sequestrating iron, leading to limit bacterial growth. -
Tissue specificity
Expressed in bone marrow and in tissues that are prone to exposure to microorganism. High expression is found in bone marrow as well as in uterus, prostate, salivary gland, stomach, appendix, colon, trachea and lung. Not found in the small intestine or peripheral blood leukocytes. -
Sequence similarities
Belongs to the calycin superfamily. Lipocalin family. -
Cellular localization
Secreted. Upon binding to the SLC22A17 (24p3R) receptor, it is internalized. - Information by UniProt
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Alternative names
- 24p3
- 25 kDa alpha-2-microglobulin-related subunit of MMP-9
- HNL
see all -
Database links
- Entrez Gene: 16819 Mouse
- SwissProt: P11672 Mouse
- Unigene: 9537 Mouse
Associated products
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SimpleStep ELISA kits
Applications
Our Abpromise guarantee covers the use of ab119601 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Sandwich ELISA | Use at an assay dependent concentration. |
Images
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ELISA: Lipocalin-2 (NGAL) Mouse ELISA Kit (ab119601)Lipocalin-2 measured in biological fluids (dilution range 1/50-1/500; duplicates +/- SD).
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ELISA: Lipocalin-2 (NGAL) Mouse ELISA Kit (ab119601)Mouse lipocalin-2 measured in tissue lysates (10-50 ug protein per mL tested; presented as NGAL per mg of extracted protein; duplicates +/- SD).
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ELISA: Lipocalin-2 (NGAL) Mouse ELISA Kit (ab119601)Lipocalin-2 measured in culture supernatants (dilution range 1/1-1/25) from control (-) or activated J774 cells ((PHA) 48 hours with 2% PHA-M; LifeTechnologies) and from RAW264 cells stimulated for 24 hours with 50 ng x mL-1 of PMA (ab120297) with the addition of 1 ug x mL-1 of LPS (Sigma) for the last 6 hours (PMA/LPS) (duplicates +/- SD).
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Typical Standard CurveRepresentative Standard Curve using ab119601
Protocols
References
This product has been referenced in:
- Gibbs KM et al. Effects of General Anesthesia on 2 Urinary Biomarkers of Kidney Injury-Hepatitis A Virus Cellular Receptor 1 and Lipocalin 2-in Male C57BL/6J Mice. J Am Assoc Lab Anim Sci 58:21-29 (2019). Read more (PubMed: 30538007) »
- Jackson EK et al. Renal 2',3'-Cyclic Nucleotide 3'-Phosphodiesterase Is an Important Determinant of AKI Severity after Ischemia-Reperfusion. J Am Soc Nephrol 27:2069-81 (2016). Read more (PubMed: 26574047) »
