Mouse Lipocalin-2 ELISA Kit (NGAL) (ab119601)
- Datasheet
- Specific References
- Protocols
Overview
-
Product nameMouse Lipocalin-2 ELISA Kit (NGAL)
See all Lipocalin-2 / NGAL kits -
Detection methodColorimetric
-
Sample typeCell culture supernatant, Urine, Serum, Plasma
-
Assay typeSandwich (quantitative)
-
Sensitivity< 10 pg/ml
-
Range78 pg/ml - 5000 pg/ml
-
Assay durationMultiple steps standard assay
-
Species reactivityReacts with: Mouse
-
Product overview
Abcam’s mouse Lipocalin-2 (NGAL) in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of mouse Lipocalin-2 in cell culture supernatants, serum, plasma (heparin) and urine. (Genecards: Lcn2)
A Lipocalin-2 specific rat monoclonal antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells and incubated. A biotinylated detection polyclonal antibody from goat specific for Lipocalin-2 is then added followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. TMB is then used to visualize the HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the mouse Lipocalin-2 amount of sample captured in plate.
Get higher sensitivity in only 90 minutes with Mouse Lipocalin-2 ELISA Kit (NGAL) (ab199083) from our SimpleStep ELISA® range.
-
Tested applicationsSuitable for: Sandwich ELISAmore details
-
PlatformMicroplate
Properties
-
Storage instructionsStore at -20°C. Please refer to protocols.
-
Components 1 x 96 tests ABC Diluent Buffer 1 x 12ml Antibody Diluent Buffer 1 x 12ml Anti-mouse Lipocalin-2 antibody Microplate (12 x 8 wells) 1 unit Avidin-Biotin-Peroxidase Complex (ABC) 1 x 130µl Biotinylated anti-mouse Lipocalin-2 antibody 1 x 130µl Lyophilized recombinant mouse Lipocalin-2 standard 2 x 10ng Plate Seal 4 units Sample Diluent Buffer 1 x 30ml TMB Color Developing Agent 1 x 10ml TMB Stop Solution 1 x 10ml -
Research areas
-
FunctionIron-trafficking protein involved in multiple processes such as apoptosis, innate immunity and renal development. Binds iron through association with 2,5-dihydroxybenzoic acid (2,5-DHBA), a siderophore that shares structural similarities with bacterial enterobactin, and delivers or removes iron from the cell, depending on the context. Iron-bound form (holo-24p3) is internalized following binding to the SLC22A17 (24p3R) receptor, leading to release of iron and subsequent increase of intracellular iron concentration. In contrast, association of the iron-free form (apo-24p3) with the SLC22A17 (24p3R) receptor is followed by association with an intracellular siderophore, iron chelation and iron transfer to the extracellular medium, thereby reducing intracellular iron concentration. Involved in apoptosis due to interleukin-3 (IL3) deprivation: iron-loaded form increases intracellular iron concentration without promoting apoptosis, while iron-free form decreases intracellular iron levels, inducing expression of the proapoptotic protein BCL2L11/BIM, resulting in apoptosis. Involved in innate immunity, possibly by sequestrating iron, leading to limit bacterial growth.
-
Tissue specificityExpressed in bone marrow and in tissues that are prone to exposure to microorganism. High expression is found in bone marrow as well as in uterus, prostate, salivary gland, stomach, appendix, colon, trachea and lung. Not found in the small intestine or peripheral blood leukocytes.
-
Sequence similaritiesBelongs to the calycin superfamily. Lipocalin family.
-
Cellular localizationSecreted. Upon binding to the SLC22A17 (24p3R) receptor, it is internalized.
- Information by UniProt
-
Alternative names
- 24p3
- 25 kDa alpha-2-microglobulin-related subunit of MMP-9
- HNL
see all -
Database links
- Entrez Gene: 16819 Mouse
- SwissProt: P11672 Mouse
- Unigene: 9537 Mouse
Associated products
-
SimpleStep ELISA kits
Applications
Our Abpromise guarantee covers the use of ab119601 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Sandwich ELISA | Use at an assay dependent concentration. |
Images
-
ELISA: Lipocalin-2 (NGAL) Mouse ELISA Kit (ab119601)Lipocalin-2 measured in biological fluids (dilution range 1/50-1/500; duplicates +/- SD).
-
ELISA: Lipocalin-2 (NGAL) Mouse ELISA Kit (ab119601)Mouse lipocalin-2 measured in tissue lysates (10-50 ug protein per mL tested; presented as NGAL per mg of extracted protein; duplicates +/- SD).
-
ELISA: Lipocalin-2 (NGAL) Mouse ELISA Kit (ab119601)Lipocalin-2 measured in culture supernatants (dilution range 1/1-1/25) from control (-) or activated J774 cells ((PHA) 48 hours with 2% PHA-M; LifeTechnologies) and from RAW264 cells stimulated for 24 hours with 50 ng x mL-1 of PMA (ab120297) with the addition of 1 ug x mL-1 of LPS (Sigma) for the last 6 hours (PMA/LPS) (duplicates +/- SD).
-
Typical Standard CurveRepresentative Standard Curve using ab119601
Protocols
References
ab119601 has not yet been referenced specifically in any publications.
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"