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Immunology Adaptive Immunity T Cells Non-CD
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Recombinant

Mouse Lipocalin-2 Matched Antibody Pair Kit (ab214477)

  • Datasheet
  • SDS
  • Protocol Booklet
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Mouse Lipocalin-2 standard curve.
  • Sandwich ELISA - Mouse Lipocalin-2 Matched Antibody Pair Kit (ab214477)

Key features and details

  • Unlabeled capture antibody, biotin-labeled detection antibody and calibrated protein standard
  • For economical ELISA and ELISA-based assay development
  • Reacts with: Mouse
  • Range: 1.95 pg/ml - 125 pg/ml

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Pair
ELISA Accessory Pack (ab210905)
Protein
Recombinant Mouse Lipocalin-2 / NGAL protein (ab95008)

View more associated products

Overview

  • Product name

    Mouse Lipocalin-2 Matched Antibody Pair Kit
    See all Lipocalin-2 / NGAL kits
  • Detection method

    Colorimetric
  • Assay type

    ELISA set
  • Sensitivity

    0.68 pg/ml
  • Range

    1.95 pg/ml - 125 pg/ml
  • Species reactivity

    Reacts with: Mouse
  • Product overview

    Mouse Lipocalin-2 Matched Antibody Pair Kits include a capture and a biotinylated detector antibody pair, along with a calibrated protein standard, suitable for sandwich ELISA. The Matched Antibody Pair Kit can be used to quantify native and recombinant mouse Lipocalin-2.


    Optimization of the kit reagents to sample type, immunoassay format or instrumentation may be required. Guidelines for use of this kit in a standard 96-well microplate sandwich ELISA using HRP/TMB system of colorimetric detection is described in this assay procedure for the purposes of quantification.


    Protocol information and tips on the use of the Matched Antibody Pair kits for sandwich ELISA can be found on our website. An accessory pack can be purchased which includes buffer reagents required to perform 10 x 96-well plate sandwich ELISAs (ab210905).


    For additional information on the performance of the antibody pair used in this kit, please see our equivalent SimpleStep ELISA kit ab199083. Please note that while the antibody pair is the same provided in the corresponding SimpleStep ELISA Kit, due to differences in their formulation, this antibody pair cannot be used with the consumables provided with our SimpleStep ELISA Kits.

  • Tested applications

    Suitable for: ELISA, IAmore details
  • Platform

    Reagents

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components 10 x 96 tests 5 x 96 tests
    Mouse Lipocalcin-2 Capture Antibody 2 x 50µg 1 x 50µg
    Mouse Lipocalcin-2 Detector Antibody 2 x 12.5µg 1 x 12.5µg
    Mouse Lipocalcin-2 Lyophilized Protein 2 vials 1 vial
  • Research areas

    • Immunology
    • Adaptive Immunity
    • T Cells
    • Non-CD
    • Cardiovascular
    • Lipids / Lipoproteins
    • Fatty Acids
    • Binding proteins
    • Signal Transduction
    • Metabolism
    • Lipid metabolism
    • Cancer
    • Tumor biomarkers
    • Other
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Lipid and lipoprotein metabolism
    • Fatty acids
    • Metabolism
    • Pathways and Processes
    • Redox metabolism
    • Fatty acid oxidation
  • Function

    Iron-trafficking protein involved in multiple processes such as apoptosis, innate immunity and renal development. Binds iron through association with 2,5-dihydroxybenzoic acid (2,5-DHBA), a siderophore that shares structural similarities with bacterial enterobactin, and delivers or removes iron from the cell, depending on the context. Iron-bound form (holo-24p3) is internalized following binding to the SLC22A17 (24p3R) receptor, leading to release of iron and subsequent increase of intracellular iron concentration. In contrast, association of the iron-free form (apo-24p3) with the SLC22A17 (24p3R) receptor is followed by association with an intracellular siderophore, iron chelation and iron transfer to the extracellular medium, thereby reducing intracellular iron concentration. Involved in apoptosis due to interleukin-3 (IL3) deprivation: iron-loaded form increases intracellular iron concentration without promoting apoptosis, while iron-free form decreases intracellular iron levels, inducing expression of the proapoptotic protein BCL2L11/BIM, resulting in apoptosis. Involved in innate immunity, possibly by sequestrating iron, leading to limit bacterial growth.
  • Tissue specificity

    Expressed in bone marrow and in tissues that are prone to exposure to microorganism. High expression is found in bone marrow as well as in uterus, prostate, salivary gland, stomach, appendix, colon, trachea and lung. Not found in the small intestine or peripheral blood leukocytes.
  • Sequence similarities

    Belongs to the calycin superfamily. Lipocalin family.
  • Cellular localization

    Secreted. Upon binding to the SLC22A17 (24p3R) receptor, it is internalized.
  • Target information above from: UniProt accession P80188 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Alternative names

    • 24p3
    • 25 kDa alpha 2 microglobulin related subunit of MMP9
    • 25 kDa alpha-2-microglobulin-related subunit of MMP-9
    • Alpha 2 microglobulin related protein
    • HGNC:6526
    • HNL
    • Lcn 2
    • Lcn2
    • Lipocalin-2
    • Migration stimulating factor inhibitor
    • MSFI
    • Neutrophil gelatinase associated lipocalin
    • Neutrophil gelatinase associated lipocalin precursor
    • Neutrophil gelatinase-associated lipocalin
    • NGAL
    • NGAL_HUMAN
    • Oncogene 24p3
    • Oncogenic lipocalin 24p3
    • p25
    • Siderocalin
    • siderocalin LCN2
    • SV40 induced 24P3 protein
    • Uterocalin
    see all
  • Database links

    • Entrez Gene: 16819 Mouse
    • SwissProt: P11672 Mouse
    • Unigene: 9537 Mouse

    Associated products

    • Alternative Versions

      • Mouse Lipocalin-2 Antibody Pair - BSA and Azide free (ab241714)
    • Related Products

      • ELISA Accessory Pack (ab210905)

    Applications

    Our Abpromise guarantee covers the use of ab214477 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    ELISA Use at an assay dependent concentration.
    IA Use at an assay dependent concentration.

    Images

    • Mouse Lipocalin-2 standard curve.
      Mouse Lipocalin-2 standard curve.

      Standard calibration curve. Background subtracted values are graphed.

    • Sandwich ELISA - Mouse Lipocalin-2 Matched Antibody Pair Kit (ab214477)
      Sandwich ELISA - Mouse Lipocalin-2 Matched Antibody Pair Kit (ab214477)
      To learn more about the advantages of recombinant antibodies see here.

    Protocols

    • Protocol Booklet

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
    • SDS
  • References (0)

    Publishing research using ab214477? Please let us know so that we can cite the reference in this datasheet.

    ab214477 has not yet been referenced specifically in any publications.

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